High Contiguity De Novo Genome Sequence Assembly of Trifoliate Yam (Dioscorea dumetorum) Using Long Read Sequencing

Siadjeu C, Pucker B, Viehöver P, Albach DC, Weisshaar B (2020)
Genes 11(3): 274.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Siadjeu, Christian; Pucker, BoasUniBi ; Viehöver, PriscaUniBi; Albach, Dirk C.; Weisshaar, BerndUniBi
Abstract / Bemerkung
Trifoliate yam (Dioscorea dumetorum) is one example of an orphan crop, not traded internationally. Post-harvest hardening of the tubers of this species starts within 24 h after harvesting and renders the tubers inedible. Genomic resources are required for D. dumetorum to improve breeding for non-hardening varieties as well as for other traits. We sequenced the D. dumetorum genome and generated the corresponding annotation. The two haplophases of this highly heterozygous genome were separated to a large extent. The assembly represents 485 Mbp of the genome with an N50 of over 3.2 Mbp. A total of 35,269 protein-encoding gene models as well as 9941 non-coding RNA genes were predicted, and functional annotations were assigned.
Stichworte
Genetics(clinical); Genetics
Erscheinungsjahr
2020
Zeitschriftentitel
Genes
Band
11
Ausgabe
3
Art.-Nr.
274
ISSN
2073-4425
eISSN
2073-4425
Page URI
https://pub.uni-bielefeld.de/record/2941669

Zitieren

Siadjeu C, Pucker B, Viehöver P, Albach DC, Weisshaar B. High Contiguity De Novo Genome Sequence Assembly of Trifoliate Yam (Dioscorea dumetorum) Using Long Read Sequencing. Genes. 2020;11(3): 274.
Siadjeu, C., Pucker, B., Viehöver, P., Albach, D. C., & Weisshaar, B. (2020). High Contiguity De Novo Genome Sequence Assembly of Trifoliate Yam (Dioscorea dumetorum) Using Long Read Sequencing. Genes, 11(3), 274. doi:10.3390/genes11030274
Siadjeu, C., Pucker, B., Viehöver, P., Albach, D. C., and Weisshaar, B. (2020). High Contiguity De Novo Genome Sequence Assembly of Trifoliate Yam (Dioscorea dumetorum) Using Long Read Sequencing. Genes 11:274.
Siadjeu, C., et al., 2020. High Contiguity De Novo Genome Sequence Assembly of Trifoliate Yam (Dioscorea dumetorum) Using Long Read Sequencing. Genes, 11(3): 274.
C. Siadjeu, et al., “High Contiguity De Novo Genome Sequence Assembly of Trifoliate Yam (Dioscorea dumetorum) Using Long Read Sequencing”, Genes, vol. 11, 2020, : 274.
Siadjeu, C., Pucker, B., Viehöver, P., Albach, D.C., Weisshaar, B.: High Contiguity De Novo Genome Sequence Assembly of Trifoliate Yam (Dioscorea dumetorum) Using Long Read Sequencing. Genes. 11, : 274 (2020).
Siadjeu, Christian, Pucker, Boas, Viehöver, Prisca, Albach, Dirk C., and Weisshaar, Bernd. “High Contiguity De Novo Genome Sequence Assembly of Trifoliate Yam (Dioscorea dumetorum) Using Long Read Sequencing”. Genes 11.3 (2020): 274.
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2020-03-17T09:38:08Z
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Dioscorea dumetorum genome sequence v1.0
Pucker B, Siadjeu C, Viehöver P, Albach D, Weisshaar B (2020)
Bielefeld University.

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PMID: 32143301
PubMed | Europe PMC

bioRxiv: 10.1101/2020.01.31.928630

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