High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser

Kong C, Pilger C, Hachmeister H, Wei X, Cheung TH, Lai CSW, Lee NP, Tsia KK, Wong KKY, Huser T (2020)
Light: Science & Applications 9(1): 25.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 4.33 MB
Autor*in
Kong, CihangUniBi; Pilger, ChristianUniBi; Hachmeister, HenningUniBi; Wei, Xiaoming; Cheung, Tom H.; Lai, Cora S. W.; Lee, Nikki P.; Tsia, Kevin. K.; Wong, Kenneth K. Y.; Huser, ThomasUniBi
Abstract / Bemerkung
Coherent Raman scattering (CRS) microscopy is widely recognized as a powerful tool for tackling biomedical problems based on its chemically specific label-free contrast, high spatial and spectral resolution, and high sensitivity. However, the clinical translation of CRS imaging technologies has long been hindered by traditional solid-state lasers with environmentally sensitive operations and large footprints. Ultrafast fibre lasers can potentially overcome these shortcomings but have not yet been fully exploited for CRS imaging, as previous implementations have suffered from high intensity noise, a narrow tuning range and low power, resulting in low image qualities and slow imaging speeds. Here, we present a novel high-power self-synchronized two-colour pulsed fibre laser that achieves excellent performance in terms of intensity stability (improved by 50 dB), timing jitter (24.3 fs), average power fluctuation (<0.5%), modulation depth (>20 dB) and pulse width variation (<1.8%) over an extended wavenumber range (2700–3550 cm−1). The versatility of the laser source enables, for the first time, high-contrast, fast CRS imaging without complicated noise reduction via balanced detection schemes. These capabilities are demonstrated in this work by imaging a wide range of species such as living human cells and mouse arterial tissues and performing multimodal nonlinear imaging of mouse tail, kidney and brain tissue sections by utilizing second-harmonic generation and two-photon excited fluorescence, which provides multiple optical contrast mechanisms simultaneously and maximizes the gathered information content for biological visualization and medical diagnosis. This work also establishes a general scenario for remodelling existing lasers into synchronized two-colour lasers and thus promotes a wider popularization and application of CRS imaging technologies.
Erscheinungsjahr
2020
Zeitschriftentitel
Light: Science & Applications
Band
9
Ausgabe
1
Art.-Nr.
25
ISSN
2095-5545
eISSN
2047-7538
Page URI
https://pub.uni-bielefeld.de/record/2941527

Zitieren

Kong C, Pilger C, Hachmeister H, et al. High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser. Light: Science & Applications. 2020;9(1): 25.
Kong, C., Pilger, C., Hachmeister, H., Wei, X., Cheung, T. H., Lai, C. S. W., Lee, N. P., et al. (2020). High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser. Light: Science & Applications, 9(1), 25. doi:10.1038/s41377-020-0259-2
Kong, C., Pilger, C., Hachmeister, H., Wei, X., Cheung, T. H., Lai, C. S. W., Lee, N. P., Tsia, K. K., Wong, K. K. Y., and Huser, T. (2020). High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser. Light: Science & Applications 9:25.
Kong, C., et al., 2020. High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser. Light: Science & Applications, 9(1): 25.
C. Kong, et al., “High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser”, Light: Science & Applications, vol. 9, 2020, : 25.
Kong, C., Pilger, C., Hachmeister, H., Wei, X., Cheung, T.H., Lai, C.S.W., Lee, N.P., Tsia, K.K., Wong, K.K.Y., Huser, T.: High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser. Light: Science & Applications. 9, : 25 (2020).
Kong, Cihang, Pilger, Christian, Hachmeister, Henning, Wei, Xiaoming, Cheung, Tom H., Lai, Cora S. W., Lee, Nikki P., Tsia, Kevin. K., Wong, Kenneth K. Y., and Huser, Thomas. “High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser”. Light: Science & Applications 9.1 (2020): 25.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0):
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2020-02-27T09:13:03Z
MD5 Prüfsumme
0197978108ed1464d39fe0226ed8c3a1

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 32133128
PubMed | Europe PMC

Suchen in

Google Scholar