High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism

Descloux A, Mueller M, Navikas V, Markwirth A, Van den Eynde R, Lukes T, Hübner W, Lasser T, Radenovic A, Dedecker P, Huser T (2020)
Nanophotonics 9(1): 143-148.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Descloux, Adrien; Mueller, Marcel; Navikas, Vytautas; Markwirth, AndreasUniBi; Van den Eynde, Robin; Lukes, Tomas; Hübner, WolfgangUniBi ; Lasser, Theo; Radenovic, Aleksandra; Dedecker, Peter; Huser, ThomasUniBi
Abstract / Bemerkung
Super-resolution structured illumination microscopy (SR-SIM) can be conducted at video-rate acquisition speeds when combined with high-speed spatial light modulators and sCMOS cameras, rendering it particularly suitable for live-cell imaging. If, however, three-dimensional (3D) information is desired, the sequential acquisition of vertical image stacks employed by current setups significantly slows down the acquisition process. In this work, we present a multiplane approach to SR-SIM that overcomes this slowdown via the simultaneous acquisition of multiple object planes, employing a recently introduced multiplane image splitting prism combined with highspeed SIM illumination. This strategy requires only the introduction of a single optical element and the addition of a second camera to acquire a laterally highly resolved 3D image stack. We demonstrate the performance of multiplane SIM by applying this instrument to imaging the dynamics of mitochondria in living COS-7 cells.
Stichworte
super-resolution optical microscopy; PACS10; 42.30.Wb; multiplane image; acquisition; structured illumination microscopy
Erscheinungsjahr
2020
Zeitschriftentitel
Nanophotonics
Band
9
Ausgabe
1
Seite(n)
143-148
ISSN
2192-8606
eISSN
2192-8614
Page URI
https://pub.uni-bielefeld.de/record/2940707

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Descloux A, Mueller M, Navikas V, et al. High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism. Nanophotonics. 2020;9(1):143-148.
Descloux, A., Mueller, M., Navikas, V., Markwirth, A., Van den Eynde, R., Lukes, T., Hübner, W., et al. (2020). High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism. Nanophotonics, 9(1), 143-148. doi:10.1515/nanoph-2019-0346
Descloux, A., Mueller, M., Navikas, V., Markwirth, A., Van den Eynde, R., Lukes, T., Hübner, W., Lasser, T., Radenovic, A., Dedecker, P., et al. (2020). High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism. Nanophotonics 9, 143-148.
Descloux, A., et al., 2020. High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism. Nanophotonics, 9(1), p 143-148.
A. Descloux, et al., “High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism”, Nanophotonics, vol. 9, 2020, pp. 143-148.
Descloux, A., Mueller, M., Navikas, V., Markwirth, A., Van den Eynde, R., Lukes, T., Hübner, W., Lasser, T., Radenovic, A., Dedecker, P., Huser, T.: High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism. Nanophotonics. 9, 143-148 (2020).
Descloux, Adrien, Mueller, Marcel, Navikas, Vytautas, Markwirth, Andreas, Van den Eynde, Robin, Lukes, Tomas, Hübner, Wolfgang, Lasser, Theo, Radenovic, Aleksandra, Dedecker, Peter, and Huser, Thomas. “High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism”. Nanophotonics 9.1 (2020): 143-148.
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2020-02-11T20:26:50Z
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