Dielectrophoretic analysis of the impact of isopropyl alcohol on the electric polarisability of Escherichia coli whole-cells

Epping MS, Wedde S, Grundmann A, Radukic M, Gröger H, Hummel A, Viefhues M (2020)
Analytical Bioanalytical Chemistry.

Zeitschriftenaufsatz | Englisch
 
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Abstract / Bemerkung
Whole-cell biocatalysts are versatile tools in (industrial) production processes; though, the effects that impact the efficiency are not fully understood yet. One main factor that affects whole-cell biocatalysts is the surrounding medium, which often consists of organic solvents due to low solubility of substrates in aqueous solutions. It is expected that organic solvents change the biophysical and biochemical properties of the whole-cell biocatalysts, e.g. by permeabilising the cell membrane, and thus analysis of these effects is of high importance. In this work, we present an analysis method to study the impact of organic solvents on whole-cell biocatalysts by means of dielectrophoresis. For instance we evaluate the changes of the characteristic dielectrophoretic trapping ratio induced by incubation of Escherichia coli, serving as model system, in isopropyl alcohol. Therefore, we could evaluate the impact on the electric polarisability of the cells. For this purpose a special microchannel device was designed and \textit{Escherichia coli} cells were genetically modified to reliably synthesize a green fluorescent protein. We could demonstrate that our method is capable to reveal different responses to small changes in isopropyl alcohol concentration and incubation duration. Complementary spectrophotometric UV-VIS (ultraviolet-visible light) absorbance analysis of released NAD(P)+/NAD(P)H cofactor and proteins confirmed our results. Based on our results we discuss the biophysical effects taking place during incubation.
Erscheinungsjahr
2020
Zeitschriftentitel
Analytical Bioanalytical Chemistry
ISSN
0016-1152
Page URI
https://pub.uni-bielefeld.de/record/2940492

Zitieren

Epping MS, Wedde S, Grundmann A, et al. Dielectrophoretic analysis of the impact of isopropyl alcohol on the electric polarisability of Escherichia coli whole-cells. Analytical Bioanalytical Chemistry. 2020.
Epping, M. S., Wedde, S., Grundmann, A., Radukic, M., Gröger, H., Hummel, A., & Viefhues, M. (2020). Dielectrophoretic analysis of the impact of isopropyl alcohol on the electric polarisability of Escherichia coli whole-cells. Analytical Bioanalytical Chemistry. doi:10.1007/s00216-020-02451-9
Epping, M. S., Wedde, S., Grundmann, A., Radukic, M., Gröger, H., Hummel, A., and Viefhues, M. (2020). Dielectrophoretic analysis of the impact of isopropyl alcohol on the electric polarisability of Escherichia coli whole-cells. Analytical Bioanalytical Chemistry.
Epping, M.S., et al., 2020. Dielectrophoretic analysis of the impact of isopropyl alcohol on the electric polarisability of Escherichia coli whole-cells. Analytical Bioanalytical Chemistry.
M.S. Epping, et al., “Dielectrophoretic analysis of the impact of isopropyl alcohol on the electric polarisability of Escherichia coli whole-cells”, Analytical Bioanalytical Chemistry, 2020.
Epping, M.S., Wedde, S., Grundmann, A., Radukic, M., Gröger, H., Hummel, A., Viefhues, M.: Dielectrophoretic analysis of the impact of isopropyl alcohol on the electric polarisability of Escherichia coli whole-cells. Analytical Bioanalytical Chemistry. (2020).
Epping, Miriam Sophie, Wedde, Severin, Grundmann, Armin, Radukic, Marco, Gröger, Harald, Hummel, Anke, and Viefhues, Martina. “Dielectrophoretic analysis of the impact of isopropyl alcohol on the electric polarisability of Escherichia coli whole-cells”. Analytical Bioanalytical Chemistry (2020).

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