Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding.

Dusny C, Grünberger A (2019)
Current opinion in biotechnology 63: 26-33.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Dusny, Christian; Grünberger, AlexanderUniBi
Abstract / Bemerkung
Our understanding of the microbial cell is based on averaged values from bulks. Microfluidic single-cell analysis holds the promise of understanding cellular processes from a single cell perspective. But what is needed to measure single-cell physiology and to disclose the consequences of individuality for biotechnology? Current single-cell research is not yet able to provide all the necessary insights, but innovative approaches now emerge that propel the field towards a better understanding of cellular processes via quantitative physiology. Here, we critically review novel single-cell technologies that enable us to control cellular input parameters such as environmental conditions and to measure intracellular processes, as well as novel approaches that enable for the first time to quantify non-averaged cell-specific rates and yields. Finally, we demonstrate how integrating microfluidic single-cell analysis into established population-based experimental workflows might unlock its full potential for biotechnology research in the future. Copyright © 2019 Elsevier Ltd. All rights reserved.
Erscheinungsjahr
2019
Zeitschriftentitel
Current opinion in biotechnology
Band
63
Seite(n)
26-33
ISSN
0958-1669
eISSN
1879-0429
Page URI
https://pub.uni-bielefeld.de/record/2939585

Zitieren

Dusny C, Grünberger A. Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding. Current opinion in biotechnology. 2019;63:26-33.
Dusny, C., & Grünberger, A. (2019). Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding. Current opinion in biotechnology, 63, 26-33. doi:10.1016/j.copbio.2019.11.001
Dusny, C., and Grünberger, A. (2019). Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding. Current opinion in biotechnology 63, 26-33.
Dusny, C., & Grünberger, A., 2019. Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding. Current opinion in biotechnology, 63, p 26-33.
C. Dusny and A. Grünberger, “Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding.”, Current opinion in biotechnology, vol. 63, 2019, pp. 26-33.
Dusny, C., Grünberger, A.: Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding. Current opinion in biotechnology. 63, 26-33 (2019).
Dusny, Christian, and Grünberger, Alexander. “Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding.”. Current opinion in biotechnology 63 (2019): 26-33.

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