Additive manufacturing of laminar flow cells for single-molecule experiments

Ahmadi A, Till K, Hafting Y, Schüttpelz M, Bjoras M, Glette K, Torresen J, Rowe AD, Dalhus B (2019)
SCIENTIFIC REPORTS 9(1): 16784: 16784.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Ahmadi, Arash; Till, KatharinaUniBi; Hafting, Yngve; Schüttpelz, MarkUniBi ; Bjoras, Magnar; Glette, Kyrre; Torresen, Jim; Rowe, Alexander D.; Dalhus, Bjorn
Abstract / Bemerkung
A microfluidic laminar flow cell (LFC) forms an indispensable component in single-molecule experiments, enabling different substances to be delivered directly to the point under observation and thereby tightly controlling the biochemical environment immediately surrounding single molecules. Despite substantial progress in the production of such components, the process remains relatively inefficient, inaccurate and time-consuming. Here we address challenges and limitations in the routines, materials and the designs that have been commonly employed in the field, and introduce a new generation of LFCs designed for single-molecule experiments and assembled using additive manufacturing. We present single- and multi-channel, as well as reservoir-based LFCs produced by 3D printing to perform single-molecule experiments. Using these flow cells along with optical tweezers, we show compatibility with single-molecule experiments including the isolation and manipulation of single DNA molecules either attached to the surface of a coverslip or as freely movable DNA dumbbells, as well as direct observation of protein-DNA interactions. Using additive manufacturing to produce LFCs with versatility of design and ease of production allow experimentalists to optimize the flow cells to their biological experiments and provide considerable potential for performing multi-component singlemolecule experiments.
Erscheinungsjahr
2019
Zeitschriftentitel
SCIENTIFIC REPORTS
Band
9
Ausgabe
1
Art.-Nr.
16784
Seite(n)
16784
ISSN
2045-2322
Page URI
https://pub.uni-bielefeld.de/record/2939437

Zitieren

Ahmadi A, Till K, Hafting Y, et al. Additive manufacturing of laminar flow cells for single-molecule experiments. SCIENTIFIC REPORTS. 2019;9(1):16784: 16784.
Ahmadi, A., Till, K., Hafting, Y., Schüttpelz, M., Bjoras, M., Glette, K., Torresen, J., et al. (2019). Additive manufacturing of laminar flow cells for single-molecule experiments. SCIENTIFIC REPORTS, 9(1), 16784., 16784. doi:10.1038/s41598-019-53151-z
Ahmadi, A., Till, K., Hafting, Y., Schüttpelz, M., Bjoras, M., Glette, K., Torresen, J., Rowe, A. D., and Dalhus, B. (2019). Additive manufacturing of laminar flow cells for single-molecule experiments. SCIENTIFIC REPORTS 9, 16784:16784.
Ahmadi, A., et al., 2019. Additive manufacturing of laminar flow cells for single-molecule experiments. SCIENTIFIC REPORTS, 9(1), p 16784: 16784.
A. Ahmadi, et al., “Additive manufacturing of laminar flow cells for single-molecule experiments”, SCIENTIFIC REPORTS, vol. 9, 2019, pp. 16784, : 16784.
Ahmadi, A., Till, K., Hafting, Y., Schüttpelz, M., Bjoras, M., Glette, K., Torresen, J., Rowe, A.D., Dalhus, B.: Additive manufacturing of laminar flow cells for single-molecule experiments. SCIENTIFIC REPORTS. 9, 16784 : 16784 (2019).
Ahmadi, Arash, Till, Katharina, Hafting, Yngve, Schüttpelz, Mark, Bjoras, Magnar, Glette, Kyrre, Torresen, Jim, Rowe, Alexander D., and Dalhus, Bjorn. “Additive manufacturing of laminar flow cells for single-molecule experiments”. SCIENTIFIC REPORTS 9.1 (2019): 16784: 16784.

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