PUB - Publikationen an der Universität Bielefeld

A Gram-strain positive, mycelium-forming actinomycete, YIM 121212T, was isolated from an alkaline soil sample collected in Yunnan province, PR China. Classification using a polyphasic approach indicated that YIM 121212T represents a member of the genus Prauserella, and is closely related to Prauserella coralliicola SCSIO 11529T (99.31%), Prauserella endophytica SP28S-3T (99.17%), Prauserella soli 12-833T (97.43%), Prauserella oleivorans RIPIT (97.03%), Prauserella marina MS498T (96.74%), Prauserella rugosa DSM 43194T (96.54%) and Prauserella muralis 05-Be-005T (95.92%). Average nucleotide identity values (ANI) of YIM 121212T to P. coralliicola DSM 45821T and P. endophytica CGMCC 4.7182T were 93.1 and 92.8 %, respectively, which were lower than the threshold of 95%. The digital DNA-DNA hybridization (dDDH) values between YIM 121212T and these two species were 50.8 and 49.9 %, respectively and thus were also well below the cut off value (>70 %) for species delineation. The DNA G+C content of YIM 121212T is 70.8mol%. Major fatty acids are iso-C16 : 0, iso-C16 : 1H, C16 : 1omega7c/iso-C15 : 0 2OH, C17 : 1omega6c, and C17 : 1omega8c. The predominant menaquinone is MK-9(H4). The polar lipid profile consists of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylmethylethanolamine (PME), phosphatidylinositol (PI), and phosphatidylinositol mannoside (PIM). The draft genomes were further analyzed for the presence of secondary metabolite biosynthesis (SMB) gene clusters. On the basis of the above observations, YIM121212T can be distinguished from closely related species belonging to the genus Prauserella. Thus, YIM121212T represents a novel species of the genus Prauserella, for which the name Prauserella flavalba sp. nov. is proposed. The type strain is YIM121212T (=CCTCCAA 2013011T=DSM 45973T).