The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis

Schuermann M, Oppel F, Gottschalk M, Büker B, Jantos CA, Knabbe C, Hütten A, Kaltschmidt B, Kaltschmidt C, Sudhoff H (2019)
FRONTIERS IN MICROBIOLOGY 10: 2325.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
 
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Autor/in
Schuermann, Matthias; Oppel, Felix; Gottschalk, MartinUniBi; Büker, BjörnUniBi; Jantos, Christian Andreas; Knabbe, Cornelius; Hütten, AndreasUniBi; Kaltschmidt, BarbaraUniBi; Kaltschmidt, ChristianUniBi; Sudhoff, Holger
Abstract / Bemerkung
Chronic rhinosinusitis (CRS) is marked by an inflamed mucosa of sinuses and is accompanied by a significantly reduced quality of live. Since no guidelines for the treatment of CRS are available, long lasting clinical histories with health care costs adding up to dozens of billion $ annually are caused by CRS. The progression of CRS is often induced by bacterial infections and/or a shift in microbiome as well as biofilm formation. The exact microbiome alterations are still unclear and the impenetrable biofilm renders the treatment with common antibiotics ineffective. This study focuses on characterizing the microbiome changes in CRS and investigating the inhibition of biofilm growth by 1,8-Cineol, a small, non-polar and hence biofilm penetrating molecule with known antimicrobial potential. We performed MALDI-TOF MS based characterization of the microbiomes of healthy individuals and CRS patients (n = 50). The microbiome in our test group was shifted to pathogens (Staphylococcus aureus, Escherichia coli, and Moraxella catarrhalis). In contrast to published studies, solely based on cell culture techniques, we could not verify the abundance of Pseudomonas aeruginosa in CRS. The inhibition of bacterial proliferation and biofilm growth by 1,8-Cineol was measured for these three pathogens. Interestingly, S. aureus, the most prominent germ in CRS, showed a biofilm inhibition not simply correlated to its inhibition of proliferation. RT-qPCR confirmed that this was due to the downregulations of major key players in biofilm generation (agrA, SarA and sigma(B)) by 1,8-Cineol. Furthermore we verified this high biofilm inhibition potential in a model host system consisting out of S. aureus biofilm grown on mature respiratory epithelium. A second host model, comprising organotypic slices, was utilized to investigate the reaction of the innate immune system present in the nasal mucosa upon biofilm formation and treatment with 1,8-Cineol. Interestingly Staphylococcus epidermidis, the cause of very common catheter infections, possesses a biofilm generation pathway very similar to S. aureus and might be treatable in a similar fashion. The two presented in vitro model systems might be transferred to combinations of every biofilm forming bacterial with most kind of epithelium and mucosa.
Stichworte
biofilm; chronic rhinosinusitis; S. aureus; host model system; 1; 8-Cineol
Erscheinungsjahr
2019
Zeitschriftentitel
FRONTIERS IN MICROBIOLOGY
Band
10
Art.-Nr.
2325
ISSN
1664-302X
Page URI
https://pub.uni-bielefeld.de/record/2938747

Zitieren

Schuermann M, Oppel F, Gottschalk M, et al. The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis. FRONTIERS IN MICROBIOLOGY. 2019;10: 2325.
Schuermann, M., Oppel, F., Gottschalk, M., Büker, B., Jantos, C. A., Knabbe, C., Hütten, A., et al. (2019). The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis. FRONTIERS IN MICROBIOLOGY, 10, 2325. doi:10.3389/fmicb.2019.02325
Schuermann, M., Oppel, F., Gottschalk, M., Büker, B., Jantos, C. A., Knabbe, C., Hütten, A., Kaltschmidt, B., Kaltschmidt, C., and Sudhoff, H. (2019). The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis. FRONTIERS IN MICROBIOLOGY 10:2325.
Schuermann, M., et al., 2019. The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis. FRONTIERS IN MICROBIOLOGY, 10: 2325.
M. Schuermann, et al., “The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis”, FRONTIERS IN MICROBIOLOGY, vol. 10, 2019, : 2325.
Schuermann, M., Oppel, F., Gottschalk, M., Büker, B., Jantos, C.A., Knabbe, C., Hütten, A., Kaltschmidt, B., Kaltschmidt, C., Sudhoff, H.: The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis. FRONTIERS IN MICROBIOLOGY. 10, : 2325 (2019).
Schuermann, Matthias, Oppel, Felix, Gottschalk, Martin, Büker, Björn, Jantos, Christian Andreas, Knabbe, Cornelius, Hütten, Andreas, Kaltschmidt, Barbara, Kaltschmidt, Christian, and Sudhoff, Holger. “The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis”. FRONTIERS IN MICROBIOLOGY 10 (2019): 2325.

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