Size-Dependent Cellular Uptake of RGD Peptides

Kemker I, Feiner R, Müller K, Sewald N (2019)
ChemBioChem.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck| Englisch
 
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Abstract / Bemerkung
Monomeric RGD peptides show unspecific fluid-phase uptake in cells, whereas multimeric RGD peptides are thought to be internalized by integrin-mediated endocytosis. However, a potential correlation between uptake mechanism and molecular mass has been neglected so far. A dual derivatization of peptide c(RGDw(7Br)K) was performed to investigate this. A fluorescent probe was installed by chemoselective Suzuki-Miyaura cross-coupling of the 7-bromotryptophan and a poly(ethylene glycol) (PEG) linker was attached to the lysine residue. Flow cytometry and live cell imaging confirmed unspecific uptake of the small, non-PEGylated peptide, whereas the PEG(5000) peptide conjugate unveiled a selective internalization by M21 cells overexpressing alpha(v)beta(3) and no uptake in alpha(v)-deficient M21L cells.
Stichworte
halogenated tryptophan; internalization; poly(ethylene glycol); RGD; peptides; selective uptake
Erscheinungsjahr
2019
Zeitschriftentitel
ChemBioChem
ISSN
1439-4227
eISSN
1439-7633
Page URI
https://pub.uni-bielefeld.de/record/2938746

Zitieren

Kemker I, Feiner R, Müller K, Sewald N. Size-Dependent Cellular Uptake of RGD Peptides. ChemBioChem. 2019.
Kemker, I., Feiner, R., Müller, K., & Sewald, N. (2019). Size-Dependent Cellular Uptake of RGD Peptides. ChemBioChem. doi:10.1002/cbic.201900512
Kemker, I., Feiner, R., Müller, K., and Sewald, N. (2019). Size-Dependent Cellular Uptake of RGD Peptides. ChemBioChem.
Kemker, I., et al., 2019. Size-Dependent Cellular Uptake of RGD Peptides. ChemBioChem.
I. Kemker, et al., “Size-Dependent Cellular Uptake of RGD Peptides”, ChemBioChem, 2019.
Kemker, I., Feiner, R., Müller, K., Sewald, N.: Size-Dependent Cellular Uptake of RGD Peptides. ChemBioChem. (2019).
Kemker, Isabell, Feiner, Rebecca, Müller, Kristian, and Sewald, Norbert. “Size-Dependent Cellular Uptake of RGD Peptides”. ChemBioChem (2019).

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PMID: 31478590
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