Size-Dependent Cellular Uptake of RGD Peptides

Kemker I, Feiner R, Müller K, Sewald N (2020)
ChemBioChem 21(4): 496-499.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Monomeric RGD peptides show unspecific fluid-phase uptake in cells, whereas multimeric RGD peptides are thought to be internalized by integrin-mediated endocytosis. However, a potential correlation between uptake mechanism and molecular mass has been neglected so far. A dual derivatization of peptide c(RGDw(7Br)K) was performed to investigate this. A fluorescent probe was installed by chemoselective Suzuki-Miyaura cross-coupling of the 7-bromotryptophan and a poly(ethylene glycol) (PEG) linker was attached to the lysine residue. Flow cytometry and live cell imaging confirmed unspecific uptake of the small, non-PEGylated peptide, whereas the PEG(5000) peptide conjugate unveiled a selective internalization by M21 cells overexpressing alpha(v)beta(3) and no uptake in alpha(v)-deficient M21L cells.
Stichworte
halogenated tryptophan; internalization; poly(ethylene glycol); RGD; peptides; selective uptake
Erscheinungsjahr
2020
Zeitschriftentitel
ChemBioChem
Band
21
Ausgabe
4
Seite(n)
496-499
ISSN
1439-4227
eISSN
1439-7633
Page URI
https://pub.uni-bielefeld.de/record/2938746

Zitieren

Kemker I, Feiner R, Müller K, Sewald N. Size-Dependent Cellular Uptake of RGD Peptides. ChemBioChem. 2020;21(4):496-499.
Kemker, I., Feiner, R., Müller, K., & Sewald, N. (2020). Size-Dependent Cellular Uptake of RGD Peptides. ChemBioChem, 21(4), 496-499. doi:10.1002/cbic.201900512
Kemker, I., Feiner, R., Müller, K., and Sewald, N. (2020). Size-Dependent Cellular Uptake of RGD Peptides. ChemBioChem 21, 496-499.
Kemker, I., et al., 2020. Size-Dependent Cellular Uptake of RGD Peptides. ChemBioChem, 21(4), p 496-499.
I. Kemker, et al., “Size-Dependent Cellular Uptake of RGD Peptides”, ChemBioChem, vol. 21, 2020, pp. 496-499.
Kemker, I., Feiner, R., Müller, K., Sewald, N.: Size-Dependent Cellular Uptake of RGD Peptides. ChemBioChem. 21, 496-499 (2020).
Kemker, Isabell, Feiner, Rebecca, Müller, Kristian, and Sewald, Norbert. “Size-Dependent Cellular Uptake of RGD Peptides”. ChemBioChem 21.4 (2020): 496-499.
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