Improved astaxanthin production with Corynebacterium glutamicum by application of a membrane fusion protein

Wendisch V, Henke NA (2019)
Marine Drugs 17: 621.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
 
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Abstract / Bemerkung
Astaxanthin is one of the strongest natural antioxidants and a red pigment occurring in nature. This C40 carotenoid is used in a broad range of applications such as a colorant in the feed industry, an antioxidant in cosmetics or as a supplement in human nutrition. Natural astaxanthin is on the rise and, hence, alternative production systems are needed. The natural carotenoid producer Corynebacterium glutamicum is a potent host for industrial fermentations, such as million-ton scale amino acid production. In C. glutamicum, astaxanthin production was established through heterologous overproduction of the cytosolic lycopene cyclase CrtY and the membrane-bound β-carotene hydroxylase and ketolase, CrtZ and CrtW, in previous studies. In this work, further metabolic engineering strategies revealed that the potential of this GRAS organism for astaxanthin production is not fully exploited yet. It was shown that the construction of a fusion protein comprising the membrane-bound β-carotene hydroxylase and ketolase (CrtZ~W) significantly increased astaxanthin production under high glucose concentration. An evaluation of used carbon sources indicated that a combination of glucose and acetate facilitated astaxanthin production. Moreover, additional overproduction of cytosolic carotenogenic enzymes increased the production of this high value compound. Taken together, a seven-fold improvement of astaxanthin production was achieved with 3.1 mg/g CDW of astaxanthin.
Stichworte
astaxanthin; Corynebacterium glutamicum; fusion protein; β-carotene ketolase; β-carotene hydroxylase
Erscheinungsjahr
2019
Zeitschriftentitel
Marine Drugs
Band
17
Seite(n)
621
eISSN
1660-3397
Finanzierungs-Informationen
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
Page URI
https://pub.uni-bielefeld.de/record/2938470

Zitieren

Wendisch V, Henke NA. Improved astaxanthin production with Corynebacterium glutamicum by application of a membrane fusion protein. Marine Drugs. 2019;17:621.
Wendisch, V., & Henke, N. A. (2019). Improved astaxanthin production with Corynebacterium glutamicum by application of a membrane fusion protein. Marine Drugs, 17, 621. doi:10.3390/md17110621
Wendisch, V., and Henke, N. A. (2019). Improved astaxanthin production with Corynebacterium glutamicum by application of a membrane fusion protein. Marine Drugs 17, 621.
Wendisch, V., & Henke, N.A., 2019. Improved astaxanthin production with Corynebacterium glutamicum by application of a membrane fusion protein. Marine Drugs, 17, p 621.
V. Wendisch and N.A. Henke, “Improved astaxanthin production with Corynebacterium glutamicum by application of a membrane fusion protein”, Marine Drugs, vol. 17, 2019, pp. 621.
Wendisch, V., Henke, N.A.: Improved astaxanthin production with Corynebacterium glutamicum by application of a membrane fusion protein. Marine Drugs. 17, 621 (2019).
Wendisch, Volker, and Henke, Nadja Alina. “Improved astaxanthin production with Corynebacterium glutamicum by application of a membrane fusion protein”. Marine Drugs 17 (2019): 621.
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2019-11-04T07:47:34Z
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