Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled.

Moritzer A-C, Niemann H (2019)
Protein science 28(12): 2112-2118.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Flavin-dependent halogenases require FADH2 , O2 and halide salts to halogenate their substrates. We describe crystal structures of the tryptophan 6-halogenase Thal in complex with FAD or with both tryptophan and FAD. If tryptophan and FAD were soaked simultaneously both ligands showed impaired binding and in some cases only the AMP or the adenosine moiety of FAD was resolved, suggesting that tryptophan binding mainly increases mobility of the FMN moiety. This confirms a negative cooperativity between substrate and cofactor binding previously described for other tryptophan halogenases. Binding of substrate to tryptophan halogenases reduces the affinity for the oxidized cofactor FAD presumably to facilitate regeneration of FADH2 by flavin reductases. This article is protected by copyright. All rights reserved. © 2019 The Protein Society.
Erscheinungsjahr
2019
Zeitschriftentitel
Protein science
Band
28
Ausgabe
12
Seite(n)
2112-2118
ISSN
0961-8368
eISSN
1469-896X
Page URI
https://pub.uni-bielefeld.de/record/2938367

Zitieren

Moritzer A-C, Niemann H. Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled. Protein science . 2019;28(12):2112-2118.
Moritzer, A. - C., & Niemann, H. (2019). Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled. Protein science , 28(12), 2112-2118. doi:10.1002/pro.3739
Moritzer, A. - C., and Niemann, H. (2019). Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled. Protein science 28, 2112-2118.
Moritzer, A.-C., & Niemann, H., 2019. Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled. Protein science , 28(12), p 2112-2118.
A.-C. Moritzer and H. Niemann, “Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled.”, Protein science , vol. 28, 2019, pp. 2112-2118.
Moritzer, A.-C., Niemann, H.: Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled. Protein science . 28, 2112-2118 (2019).
Moritzer, Ann-Christin, and Niemann, Hartmut. “Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled.”. Protein science 28.12 (2019): 2112-2118.
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2020-03-13T13:16:21Z
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