Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled.

Moritzer A-C, Niemann H (2019)
Protein science : a publication of the Protein Society.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck| Englisch
 
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Abstract / Bemerkung
Flavin-dependent halogenases require FADH2 , O2 and halide salts to halogenate their substrates. We describe crystal structures of the tryptophan 6-halogenase Thal in complex with FAD or with both tryptophan and FAD. If tryptophan and FAD were soaked simultaneously both ligands showed impaired binding and in some cases only the AMP or the adenosine moiety of FAD was resolved, suggesting that tryptophan binding mainly increases mobility of the FMN moiety. This confirms a negative cooperativity between substrate and cofactor binding previously described for other tryptophan halogenases. Binding of substrate to tryptophan halogenases reduces the affinity for the oxidized cofactor FAD presumably to facilitate regeneration of FADH2 by flavin reductases. This article is protected by copyright. All rights reserved. © 2019 The Protein Society.
Erscheinungsjahr
2019
Zeitschriftentitel
Protein science : a publication of the Protein Society
ISSN
1469-896X
Page URI
https://pub.uni-bielefeld.de/record/2938367

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Moritzer A-C, Niemann H. Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled. Protein science : a publication of the Protein Society. 2019.
Moritzer, A. - C., & Niemann, H. (2019). Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled. Protein science : a publication of the Protein Society. doi:10.1002/pro.3739
Moritzer, A. - C., and Niemann, H. (2019). Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled. Protein science : a publication of the Protein Society.
Moritzer, A.-C., & Niemann, H., 2019. Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled. Protein science : a publication of the Protein Society.
A.-C. Moritzer and H. Niemann, “Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled.”, Protein science : a publication of the Protein Society, 2019.
Moritzer, A.-C., Niemann, H.: Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled. Protein science : a publication of the Protein Society. (2019).
Moritzer, Ann-Christin, and Niemann, Hartmut. “Binding of FAD and tryptophan to the tryptophan 6-halogenase Thal are negatively coupled.”. Protein science : a publication of the Protein Society (2019).

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