Functional Evolution of Nodulin 26-like Intrinsic Proteins: From bacterial arsenic detoxification to plant nutrient transport

Pommerrenig B, Diehn TA, Bernhardt N, Bienert MD, Mitani-Ueno N, Fuge J, Bieber A, Spitzer C, Bräutigam A, Ma JF, Chaumont F, et al. (2020)
New Phytologist 225(3): 1383-1396.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Pommerrenig, Benjamin; Diehn, Till A.; Bernhardt, Nadine; Bienert, Manuela D.; Mitani-Ueno, Namiki; Fuge, Jacqueline; Bieber, Annett; Spitzer, Christoph; Bräutigam, AndreaUniBi ; Ma, Jian Feng; Chaumont, Francois; Bienert, Gerd P.
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Abstract / Bemerkung
Nodulin 26-like-intrinsic-proteins (NIPs) play essential roles in transporting the nutrients, silicon and boron, in seed plants, but the evolutionary origin of this transport function and the co-permeability to toxic arsenic remains enigmatic. Horizontal gene transfer of a yet uncharacterized bacterial AqpN-aquaporin group was the starting-point for plant NIP evolution. We combined intense sequence-, phylogenetic and genetic context analyses and a mutational approach with various transport assays in oocytes and plants to resolve the trans-organismal and functional evolution of bacterial and algal and terrestrial plant NIPs and to reveal their molecular transport specificity features. We discovered that aqpN genes are prevalently located in arsenic-resistance-operons of various prokaryotic phyla. We provided genetic and functional evidence that these proteins contribute to the arsenic detoxification machinery. We identified NIPs with the ancestral bacterial AqpN selectivity filter composition in algae, liverworts, moss, hornworts and ferns and demonstrated that these archetype plant NIPs and their prokaryotic progenitors are almost impermeable to water and silicon but transport arsenic and boron. With a mutational approach, we demonstrated that during evolution, ancestral NIP selectivity shifted to allow subfunctionalizations. Together, our data provided evidence that evolution converted bacterial arsenic efflux channels into essential seed plant nutrient transporters. © 2019 The Authors New Phytologist © 2019 New Phytologist Trust.
Erscheinungsjahr
2020
Zeitschriftentitel
New Phytologist
Band
225
Ausgabe
3
Seite(n)
1383-1396
ISSN
0028-646x
eISSN
1469-8137
Page URI
https://pub.uni-bielefeld.de/record/2937751

Zitieren

Pommerrenig B, Diehn TA, Bernhardt N, et al. Functional Evolution of Nodulin 26-like Intrinsic Proteins: From bacterial arsenic detoxification to plant nutrient transport. New Phytologist. 2020;225(3):1383-1396.
Pommerrenig, B., Diehn, T. A., Bernhardt, N., Bienert, M. D., Mitani-Ueno, N., Fuge, J., Bieber, A., et al. (2020). Functional Evolution of Nodulin 26-like Intrinsic Proteins: From bacterial arsenic detoxification to plant nutrient transport. New Phytologist, 225(3), 1383-1396. doi:10.1111/nph.16217
Pommerrenig, B., Diehn, T. A., Bernhardt, N., Bienert, M. D., Mitani-Ueno, N., Fuge, J., Bieber, A., Spitzer, C., Bräutigam, A., Ma, J. F., et al. (2020). Functional Evolution of Nodulin 26-like Intrinsic Proteins: From bacterial arsenic detoxification to plant nutrient transport. New Phytologist 225, 1383-1396.
Pommerrenig, B., et al., 2020. Functional Evolution of Nodulin 26-like Intrinsic Proteins: From bacterial arsenic detoxification to plant nutrient transport. New Phytologist, 225(3), p 1383-1396.
B. Pommerrenig, et al., “Functional Evolution of Nodulin 26-like Intrinsic Proteins: From bacterial arsenic detoxification to plant nutrient transport”, New Phytologist, vol. 225, 2020, pp. 1383-1396.
Pommerrenig, B., Diehn, T.A., Bernhardt, N., Bienert, M.D., Mitani-Ueno, N., Fuge, J., Bieber, A., Spitzer, C., Bräutigam, A., Ma, J.F., Chaumont, F., Bienert, G.P.: Functional Evolution of Nodulin 26-like Intrinsic Proteins: From bacterial arsenic detoxification to plant nutrient transport. New Phytologist. 225, 1383-1396 (2020).
Pommerrenig, Benjamin, Diehn, Till A., Bernhardt, Nadine, Bienert, Manuela D., Mitani-Ueno, Namiki, Fuge, Jacqueline, Bieber, Annett, Spitzer, Christoph, Bräutigam, Andrea, Ma, Jian Feng, Chaumont, Francois, and Bienert, Gerd P. “Functional Evolution of Nodulin 26-like Intrinsic Proteins: From bacterial arsenic detoxification to plant nutrient transport”. New Phytologist 225.3 (2020): 1383-1396.
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