Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria).

Assie A, Leisch N, Meier DV, Gruber-Vodicka H, Tegetmeyer H, Meyerdierks A, Kleiner M, Hinzke T, Joye S, Saxton M, Dubilier N, et al. (2019)
The ISME journal.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck| Englisch
 
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Autor/in
Assie, Adrien; Leisch, Nikolaus; Meier, Dimitri V; Gruber-Vodicka, Harald; Tegetmeyer, HalinaUniBi ; Meyerdierks, Anke; Kleiner, Manuel; Hinzke, Tjorven; Joye, Samantha; Saxton, Matthew; Dubilier, Nicole; Petersen, Jillian M
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Abstract / Bemerkung
Most autotrophs use the Calvin-Benson-Bassham (CBB) cycle for carbon fixation. In contrast, all currently described autotrophs from the Campylobacterota (previously Epsilonproteobacteria) use the reductive tricarboxylic acid cycle (rTCA) instead. We discovered campylobacterotal epibionts ("Candidatus Thiobarba") of deep-sea mussels that have acquired a complete CBB cycle and may have lost most key genes of the rTCA cycle. Intriguingly, the phylogenies of campylobacterotal CBB cyclegenes suggest they were acquired in multiple transfers from Gammaproteobacteria closely related to sulfur-oxidizing endosymbionts associated with the mussels, as well as from Betaproteobacteria. We hypothesize that "Ca. Thiobarba" switched from the rTCA cycle to a fully functional CBB cycle during its evolution, by acquiring genes from multiple sources, including co-occurring symbionts. We also found key CBB cycle genes in free-living Campylobacterota, suggesting that the CBB cycle may be more widespread in this phylum than previously known. Metatranscriptomics and metaproteomics confirmed high expression of CBB cycle genes in mussel-associated "Ca. Thiobarba". Direct stable isotope fingerprinting showed that "Ca. Thiobarba" has typical CBB signatures, suggesting that it uses this cycle for carbon fixation. Our discovery calls into question current assumptions about the distribution of carbon fixation pathways in microbial lineages, and the interpretation of stable isotope measurements in the environment.
Erscheinungsjahr
2019
Zeitschriftentitel
The ISME journal
eISSN
1751-7370
Page URI
https://pub.uni-bielefeld.de/record/2937747

Zitieren

Assie A, Leisch N, Meier DV, et al. Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria). The ISME journal. 2019.
Assie, A., Leisch, N., Meier, D. V., Gruber-Vodicka, H., Tegetmeyer, H., Meyerdierks, A., Kleiner, M., et al. (2019). Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria). The ISME journal. doi:10.1038/s41396-019-0508-7
Assie, A., Leisch, N., Meier, D. V., Gruber-Vodicka, H., Tegetmeyer, H., Meyerdierks, A., Kleiner, M., Hinzke, T., Joye, S., Saxton, M., et al. (2019). Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria). The ISME journal.
Assie, A., et al., 2019. Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria). The ISME journal.
A. Assie, et al., “Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria).”, The ISME journal, 2019.
Assie, A., Leisch, N., Meier, D.V., Gruber-Vodicka, H., Tegetmeyer, H., Meyerdierks, A., Kleiner, M., Hinzke, T., Joye, S., Saxton, M., Dubilier, N., Petersen, J.M.: Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria). The ISME journal. (2019).
Assie, Adrien, Leisch, Nikolaus, Meier, Dimitri V, Gruber-Vodicka, Harald, Tegetmeyer, Halina, Meyerdierks, Anke, Kleiner, Manuel, Hinzke, Tjorven, Joye, Samantha, Saxton, Matthew, Dubilier, Nicole, and Petersen, Jillian M. “Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria).”. The ISME journal (2019).

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