Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction

Markwirth A, Lachetta M, Mönkemöller V, Heintzmann R, Hübner W, Huser T, Müller M (2019)
Nature communications 10: 4315.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Super-resolved structured illumination microscopy (SR-SIM) is among the fastest fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-build instruments are able to deliver two-fold resolution enhancement with high acquisition speed. SR-SIM is usually a two-step process, with raw-data acquisition and subsequent, time-consuming post-processing for image reconstruction. In contrast, wide-field and (multi-spot) confocal techniques produce high-resolution images instantly. Such immediacy is also possible with SR-SIM, by tight integration of a video-rate capable SIM with fast reconstruction software. Here we present instant SR-SIM by VIGOR (Video-rate Immediate GPU-accelerated Open-Source Reconstruction). We demonstrate multi-color SR-SIM at video frame-rates, with less than 250ms delay between measurement and reconstructed image display. This is achieved by modifying and extending high-speed SR-SIM image acquisition with a new, GPU-enhanced, network-enabled image-reconstruction software. We demonstrate high-speed surveying of biological samples in multiple colors and live imaging of moving mitochondria as an example of intracellular dynamics.
Erscheinungsjahr
2019
Zeitschriftentitel
Nature communications
Band
10
Art.-Nr.
4315
eISSN
2041-1723
Finanzierungs-Informationen
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
Page URI
https://pub.uni-bielefeld.de/record/2937573

Zitieren

Markwirth A, Lachetta M, Mönkemöller V, et al. Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction. Nature communications. 2019;10: 4315.
Markwirth, A., Lachetta, M., Mönkemöller, V., Heintzmann, R., Hübner, W., Huser, T., & Müller, M. (2019). Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction. Nature communications, 10, 4315. doi:10.1038/s41467-019-12165-x
Markwirth, A., Lachetta, M., Mönkemöller, V., Heintzmann, R., Hübner, W., Huser, T., and Müller, M. (2019). Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction. Nature communications 10:4315.
Markwirth, A., et al., 2019. Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction. Nature communications, 10: 4315.
A. Markwirth, et al., “Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction”, Nature communications, vol. 10, 2019, : 4315.
Markwirth, A., Lachetta, M., Mönkemöller, V., Heintzmann, R., Hübner, W., Huser, T., Müller, M.: Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction. Nature communications. 10, : 4315 (2019).
Markwirth, Andreas, Lachetta, Mario, Mönkemöller, Viola, Heintzmann, Rainer, Hübner, Wolfgang, Huser, Thomas, and Müller, Marcel. “Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction”. Nature communications 10 (2019): 4315.
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2019-10-08T09:18:09Z
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