Biotransformations in pure organic medium: organic solvent-labile enzymes in batch and flow exemplified for nitrile synthesis

Hinzmann A, Adebar N, Betke T, Leppin M, Gröger H (2019)
European Journal of Organic Chemistry 2019(41): 6911-6916.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
In recent years, there has been an increasing tendency to use biocatalysts in industrial chemistry, especially in the pharma and fine chemical sector. Preferably, enzymes or whole cells, applied as catalysts for a specific biotransformation, are utilized in aqueous reaction media since water is the natural medium for enzymes. In numerous examples of biocatalytic systems, however, a major problem is the insolubility of hydrophobic substrates in such aqueous reaction media. Apart from lipases, many enzymes are highly sensitive to organic solvents and are inactivated by an organic medium. Therefore, a change of solvent for biotransformations from water to organic solvents is usually challenging. In this study, we investigated the synthesis of nitriles by an organic solvent‐labile aldoxime dehydratase in pure organic solvents, exemplified for the dehydration of n‐octanaloxime to n‐octanenitrile. We present a method for applications in batch as well as flow mode based on an “immobilized aqueous phase” bearing the whole cells in a superabsorber as solid phase, thus enabling the use of a purely organic solvent as “mobile phase” and reaction medium.
Erscheinungsjahr
2019
Zeitschriftentitel
European Journal of Organic Chemistry
Band
2019
Ausgabe
41
Seite(n)
6911-6916
eISSN
1434-193X
Page URI
https://pub.uni-bielefeld.de/record/2937513

Zitieren

Hinzmann A, Adebar N, Betke T, Leppin M, Gröger H. Biotransformations in pure organic medium: organic solvent-labile enzymes in batch and flow exemplified for nitrile synthesis. European Journal of Organic Chemistry. 2019;2019(41):6911-6916.
Hinzmann, A., Adebar, N., Betke, T., Leppin, M., & Gröger, H. (2019). Biotransformations in pure organic medium: organic solvent-labile enzymes in batch and flow exemplified for nitrile synthesis. European Journal of Organic Chemistry, 2019(41), 6911-6916. doi:10.1002/ejoc.201901168
Hinzmann, A., Adebar, N., Betke, T., Leppin, M., and Gröger, H. (2019). Biotransformations in pure organic medium: organic solvent-labile enzymes in batch and flow exemplified for nitrile synthesis. European Journal of Organic Chemistry 2019, 6911-6916.
Hinzmann, A., et al., 2019. Biotransformations in pure organic medium: organic solvent-labile enzymes in batch and flow exemplified for nitrile synthesis. European Journal of Organic Chemistry, 2019(41), p 6911-6916.
A. Hinzmann, et al., “Biotransformations in pure organic medium: organic solvent-labile enzymes in batch and flow exemplified for nitrile synthesis”, European Journal of Organic Chemistry, vol. 2019, 2019, pp. 6911-6916.
Hinzmann, A., Adebar, N., Betke, T., Leppin, M., Gröger, H.: Biotransformations in pure organic medium: organic solvent-labile enzymes in batch and flow exemplified for nitrile synthesis. European Journal of Organic Chemistry. 2019, 6911-6916 (2019).
Hinzmann, Alessa, Adebar, Niklas, Betke, Tobias, Leppin, Monja, and Gröger, Harald. “Biotransformations in pure organic medium: organic solvent-labile enzymes in batch and flow exemplified for nitrile synthesis”. European Journal of Organic Chemistry 2019.41 (2019): 6911-6916.
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2020-03-13T13:34:11Z
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