Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas putida

Mindt M, Hannibal S, Heuser M, Risse JM, Sasikumar K, Nampoothiri KM, Wendisch VF (2019)
Frontiers in Bioengineering and Biotechnology 7: 232.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Mindt, MelanieUniBi; Hannibal, SilvinUniBi; Heuser, Maria; Risse, Joe MaxUniBi; Sasikumar, Keerthi; Nampoothiri, K.Madhavan; Wendisch, Volker F.UniBi
Abstract / Bemerkung
Sarcosine, an N-methylated amino acid, shows potential as antipsychotic, and serves as building block for peptide-based drugs, and acts as detergent when acetylated. N-methylated amino acids are mainly produced chemically or by biocatalysis, with either low yields or high costs for co-factor regeneration. Corynebacterium glutamicum, which is used for the industrial production of amino acids for decades, has recently been engineered for production of N-methyl-L-alanine and sarcosine. Heterologous expression of dpkA in a C. glutamicum strain engineered for glyoxylate overproduction enabled fermentative production of sarcosine from sugars and monomethylamine. Here, mutation of an amino acyl residue in the substrate binding site of DpkA (DpkAF117L) led to an increased specific activity for reductive alkylamination of glyoxylate using monomethylamine and monoethylamine as substrates. Introduction of DpkAF117L into the production strain accelerated the production of sarcosine and a volumetric productivity of 0.16 g L−1 h−1 could be attained. Using monoethylamine as substrate, we demonstrated N-ethylglycine production with a volumetric productivity of 0.11 g L−1 h−1, which to the best of our knowledge is the first report of its fermentative production. Subsequently, the feasibility of using rice straw hydrolysate as alternative carbon source was tested and production of N-ethylglycine to a titer of 1.6 g L−1 after 60 h of fed-batch bioreactor cultivation could be attained.
Erscheinungsjahr
2019
Zeitschriftentitel
Frontiers in Bioengineering and Biotechnology
Band
7
Art.-Nr.
232
ISSN
2296-4185
eISSN
2296-4185
Page URI
https://pub.uni-bielefeld.de/record/2937220

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Mindt M, Hannibal S, Heuser M, et al. Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas putida. Frontiers in Bioengineering and Biotechnology. 2019;7: 232.
Mindt, M., Hannibal, S., Heuser, M., Risse, J. M., Sasikumar, K., Nampoothiri, K. M., & Wendisch, V. F. (2019). Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas putida. Frontiers in Bioengineering and Biotechnology, 7, 232. doi:10.3389/fbioe.2019.00232
Mindt, M., Hannibal, S., Heuser, M., Risse, J. M., Sasikumar, K., Nampoothiri, K. M., and Wendisch, V. F. (2019). Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas putida. Frontiers in Bioengineering and Biotechnology 7:232.
Mindt, M., et al., 2019. Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas putida. Frontiers in Bioengineering and Biotechnology, 7: 232.
M. Mindt, et al., “Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas putida”, Frontiers in Bioengineering and Biotechnology, vol. 7, 2019, : 232.
Mindt, M., Hannibal, S., Heuser, M., Risse, J.M., Sasikumar, K., Nampoothiri, K.M., Wendisch, V.F.: Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas putida. Frontiers in Bioengineering and Biotechnology. 7, : 232 (2019).
Mindt, Melanie, Hannibal, Silvin, Heuser, Maria, Risse, Joe Max, Sasikumar, Keerthi, Nampoothiri, K.Madhavan, and Wendisch, Volker F. “Fermentative production of N-alkylated glycine derivatives by recombinant Corynebacterium glutamicum using a mutant of imine reductase DpkA from Pseudomonas putida”. Frontiers in Bioengineering and Biotechnology 7 (2019): 232.
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2019-12-12T10:53:44Z
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