Conversion of Serine-Type Aldehyde Tags by the Radical SAM Protein AtsB from Methanosarcina mazei

Krüger T, Weiland S, Boschanski M, Sinha PK, Falck G, Müller K, Dierks T, Sewald N (2019)
ChemBioChem 20(16: Special Issue: 10th IPS): 2074-2078.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Formylglycine-generating enzymes provide a convenient tool for site-specific protein derivatization. Their ability to oxidize cysteine or serine residues within a defined consensus sequence to C-alpha-formylglycine (FGly) allows for the targeted introduction of a unique chemical handle for various bioconjugation reactions. In recent years, oxygen-dependent FGly-generating enzyme saw broad use in protein functionalization and the generation of protein conjugates. Yet, the FGly-generating system AtsB, along with its capability to convert unusual aldehyde tag sequences, remains mostly unused. Herein, the ability of AtsB from Methanosarcina mazei to convert nonclassical aldehyde tags of the SX(A/P)XR-type and its potential use in bioconjugation chemistry are demonstrated.
Stichworte
aldehydes; enzymes; formylglycine; peptides; protein engineering
Erscheinungsjahr
2019
Zeitschriftentitel
ChemBioChem
Band
20
Ausgabe
16: Special Issue: 10th IPS
Seite(n)
2074-2078
ISSN
1439-4227
eISSN
1439-7633
Page URI
https://pub.uni-bielefeld.de/record/2936936

Zitieren

Krüger T, Weiland S, Boschanski M, et al. Conversion of Serine-Type Aldehyde Tags by the Radical SAM Protein AtsB from Methanosarcina mazei. ChemBioChem . 2019;20(16: Special Issue: 10th IPS):2074-2078.
Krüger, T., Weiland, S., Boschanski, M., Sinha, P. K., Falck, G., Müller, K., Dierks, T., et al. (2019). Conversion of Serine-Type Aldehyde Tags by the Radical SAM Protein AtsB from Methanosarcina mazei. ChemBioChem , 20(16: Special Issue: 10th IPS), 2074-2078. doi:10.1002/cbic.201900322
Krüger, T., Weiland, S., Boschanski, M., Sinha, P. K., Falck, G., Müller, K., Dierks, T., and Sewald, N. (2019). Conversion of Serine-Type Aldehyde Tags by the Radical SAM Protein AtsB from Methanosarcina mazei. ChemBioChem 20, 2074-2078.
Krüger, T., et al., 2019. Conversion of Serine-Type Aldehyde Tags by the Radical SAM Protein AtsB from Methanosarcina mazei. ChemBioChem , 20(16: Special Issue: 10th IPS), p 2074-2078.
T. Krüger, et al., “Conversion of Serine-Type Aldehyde Tags by the Radical SAM Protein AtsB from Methanosarcina mazei”, ChemBioChem , vol. 20, 2019, pp. 2074-2078.
Krüger, T., Weiland, S., Boschanski, M., Sinha, P.K., Falck, G., Müller, K., Dierks, T., Sewald, N.: Conversion of Serine-Type Aldehyde Tags by the Radical SAM Protein AtsB from Methanosarcina mazei. ChemBioChem . 20, 2074-2078 (2019).
Krüger, Tobias, Weiland, Stefanie, Boschanski, Mareile, Sinha, Prem Kumar, Falck, Georg, Müller, Kristian, Dierks, Thomas, and Sewald, Norbert. “Conversion of Serine-Type Aldehyde Tags by the Radical SAM Protein AtsB from Methanosarcina mazei”. ChemBioChem 20.16: Special Issue: 10th IPS (2019): 2074-2078.

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