MoNa - a cost-efficient, portable system for the nanoinjection of living cells

Simonis M, Sandmeyer A, Greiner J, Kaltschmidt B, Huser T, Hennig S (2019)
Scientific reports 9: 5480.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
 
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Abstract / Bemerkung
Injection techniques to deliver macromolecules to cells such as microinjection have been around for decades with applications ranging from probing whole organisms to the injection of fluorescent molecules into single cells. A similar technique that has raised recent interest is nanoinjection. The pipettes used here are much smaller and allow for the precise deposition of molecules into single cells via electrokinetics with minimal influence on the cells' health. Unfortunately, the equipment utilized for nanoinjection originates from scanning ion conductance microscopy (SICM) and is therefore expensive and not portable, but usually fixed to a specific microscope setup. The level of precision that these systems achieve is much higher than what is needed for the more robust nanoinjection process. We present Mobile Nanoinjection (MoNa), a portable, cost-efficient and easy to build system for the injection of single cells. Sacrificing unnecessary sub-nanometer accuracy and low ion current noise levels, we were able to inject single living cells with high accuracy. We determined the noise of the MoNa system and investigated the injection conditions for 16 prominent fluorescent labels and fluorophores. Further, we performed proof of concepts by injection of ATTO655-Phalloidin and MitoTracker Deep Red to living human osteosarcoma (U2OS) cells and of living adult human inferior turbinate stem cells (ITSC's) following neuronal differentiation with the MoNa system. We achieved significant cost reductions of the nanoinjection technology and gained full portability and compatibility to most optical microscopes.
Erscheinungsjahr
2019
Zeitschriftentitel
Scientific reports
Band
9
Art.-Nr.
5480
ISSN
2045-2322
Finanzierungs-Informationen
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
Page URI
https://pub.uni-bielefeld.de/record/2934791

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Simonis M, Sandmeyer A, Greiner J, Kaltschmidt B, Huser T, Hennig S. MoNa - a cost-efficient, portable system for the nanoinjection of living cells. Scientific reports. 2019;9: 5480.
Simonis, M., Sandmeyer, A., Greiner, J., Kaltschmidt, B., Huser, T., & Hennig, S. (2019). MoNa - a cost-efficient, portable system for the nanoinjection of living cells. Scientific reports, 9, 5480. doi:10.1038/s41598-019-41648-6
Simonis, M., Sandmeyer, A., Greiner, J., Kaltschmidt, B., Huser, T., and Hennig, S. (2019). MoNa - a cost-efficient, portable system for the nanoinjection of living cells. Scientific reports 9:5480.
Simonis, M., et al., 2019. MoNa - a cost-efficient, portable system for the nanoinjection of living cells. Scientific reports, 9: 5480.
M. Simonis, et al., “MoNa - a cost-efficient, portable system for the nanoinjection of living cells”, Scientific reports, vol. 9, 2019, : 5480.
Simonis, M., Sandmeyer, A., Greiner, J., Kaltschmidt, B., Huser, T., Hennig, S.: MoNa - a cost-efficient, portable system for the nanoinjection of living cells. Scientific reports. 9, : 5480 (2019).
Simonis, Matthias, Sandmeyer, Alice, Greiner, Johannes, Kaltschmidt, Barbara, Huser, Thomas, and Hennig, Simon. “MoNa - a cost-efficient, portable system for the nanoinjection of living cells”. Scientific reports 9 (2019): 5480.
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