Formylglycine-generating enzymes for site-specific bioconjugation

Krüger T, Dierks T, Sewald N (2019)
BIOLOGICAL CHEMISTRY 400(3): 289-297.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
Site-specific bioconjugation strategies offer many possibilities for directed protein modifications. Among the various enzyme-based conjugation protocols, formylglycine-generating enzymes allow to posttranslationally introduce the amino acid C-alpha-formylglycine (FGly) into recombinant proteins, starting from cysteine or serine residues within distinct consensus motifs. The aldehyde-bearing FGly-residue displays orthogonal reactivity to all other natural amino acids and can, therefore, be used for site-specific labeling reactions on protein scaffolds. In this review, the state of research on catalytic mechanisms and consensus motifs of different formylglycine-generating enzymes, as well as labeling strategies and applications of FGly-based bioconjugations are presented.
Erscheinungsjahr
Zeitschriftentitel
BIOLOGICAL CHEMISTRY
Band
400
Ausgabe
3
Seite(n)
289-297
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Krüger T, Dierks T, Sewald N. Formylglycine-generating enzymes for site-specific bioconjugation. BIOLOGICAL CHEMISTRY. 2019;400(3):289-297.
Krüger, T., Dierks, T., & Sewald, N. (2019). Formylglycine-generating enzymes for site-specific bioconjugation. BIOLOGICAL CHEMISTRY, 400(3), 289-297. doi:10.1515/hsz-2018-0358
Krüger, T., Dierks, T., and Sewald, N. (2019). Formylglycine-generating enzymes for site-specific bioconjugation. BIOLOGICAL CHEMISTRY 400, 289-297.
Krüger, T., Dierks, T., & Sewald, N., 2019. Formylglycine-generating enzymes for site-specific bioconjugation. BIOLOGICAL CHEMISTRY, 400(3), p 289-297.
T. Krüger, T. Dierks, and N. Sewald, “Formylglycine-generating enzymes for site-specific bioconjugation”, BIOLOGICAL CHEMISTRY, vol. 400, 2019, pp. 289-297.
Krüger, T., Dierks, T., Sewald, N.: Formylglycine-generating enzymes for site-specific bioconjugation. BIOLOGICAL CHEMISTRY. 400, 289-297 (2019).
Krüger, Tobias, Dierks, Thomas, and Sewald, Norbert. “Formylglycine-generating enzymes for site-specific bioconjugation”. BIOLOGICAL CHEMISTRY 400.3 (2019): 289-297.

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PMID: 30291781
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