Function of L-Pipecolic acid as compatible solute in Corynebacterium glutamicum as basis for its production under hyperosmolar conditions

Perez F, Fernandes de Brito L, Wendisch VF (2019)
Frontiers in Microbiology 10: 340.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
OA 2.67 MB
Abstract / Bemerkung
Pipecolic acid or L-PA is a cyclic amino acid derived from L-lysine which has gained interest in the recent years within the pharmaceutical and chemical industries. L-PA can be produced efficiently using recombinant Corynebacterium glutamicum strains by expanding the natural L-lysine biosynthetic pathway. L-PA is a six-membered ring homolog of the five-membered ring amino acid L-proline, which serves as compatible solute in C. glutamicum. Here, we show that de novo synthesized or externally added L-PA partially is beneficial for growth under hyper-osmotic stress conditions. C. glutamicum cells accumulated L-PA under elevated osmotic pressure and released it after an osmotic down shock. In the absence of the mechanosensitive channel YggB intracellular L-PA concentrations increased and its release after osmotic down shock was slower. The proline permease ProP was identified as a candidate L-PA uptake system since RNAseq analysis revealed increased proP RNA levels upon L-PA production. Under hyper-osmotic conditions, a ΔproP strain showed similar growth behavior than the parent strain when L-proline was added externally. By contrast, the growth impairment of the ΔproP strain under hyper-osmotic conditions could not be alleviated by addition of L-PA unless proP was expressed from a plasmid. This is commensurate with the view that L-proline can be imported into the C. glutamicum cell by ProP and other transporters such as EctP and PutP, while ProP appears of major importance for L-PA uptake under hyper-osmotic stress conditions.
Frontiers in Microbiology
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Perez F, Fernandes de Brito L, Wendisch VF. Function of L-Pipecolic acid as compatible solute in Corynebacterium glutamicum as basis for its production under hyperosmolar conditions. Frontiers in Microbiology. 2019;10:340.
Perez, F., Fernandes de Brito, L., & Wendisch, V. F. (2019). Function of L-Pipecolic acid as compatible solute in Corynebacterium glutamicum as basis for its production under hyperosmolar conditions. Frontiers in Microbiology, 10, 340. doi:10.3389/fmicb.2019.00340
Perez, F., Fernandes de Brito, L., and Wendisch, V. F. (2019). Function of L-Pipecolic acid as compatible solute in Corynebacterium glutamicum as basis for its production under hyperosmolar conditions. Frontiers in Microbiology 10, 340.
Perez, F., Fernandes de Brito, L., & Wendisch, V.F., 2019. Function of L-Pipecolic acid as compatible solute in Corynebacterium glutamicum as basis for its production under hyperosmolar conditions. Frontiers in Microbiology, 10, p 340.
F. Perez, L. Fernandes de Brito, and V.F. Wendisch, “Function of L-Pipecolic acid as compatible solute in Corynebacterium glutamicum as basis for its production under hyperosmolar conditions”, Frontiers in Microbiology, vol. 10, 2019, pp. 340.
Perez, F., Fernandes de Brito, L., Wendisch, V.F.: Function of L-Pipecolic acid as compatible solute in Corynebacterium glutamicum as basis for its production under hyperosmolar conditions. Frontiers in Microbiology. 10, 340 (2019).
Perez, Fernando, Fernandes de Brito, Luciana, and Wendisch, Volker F. “Function of L-Pipecolic acid as compatible solute in Corynebacterium glutamicum as basis for its production under hyperosmolar conditions”. Frontiers in Microbiology 10 (2019): 340.
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