Technical scale production of encapsulated Saccharomyces cerevisiae and Metarhizium brunneum attractive to wireworms

Humbert P, Przyklenk M, Vemmer M, Schumann M, Vidal S, Patel A (2017)
Biocontrol Science and Technology 27(9): 1049-1070.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Humbert, Pascal; Przyklenk, Michael; Vemmer, Marina; Schumann, Mario; Vidal, Stefan; Patel, AnantUniBi
Abstract / Bemerkung
Wireworms (Coleoptera: Elateridae) have recently become an increasing problem as agricultural insect pests due to the phasing out of effective control options. Entomopathogenic fungi such as Metarhizium brunneum have proven to be a promising microbial antagonist for wireworm control. Here, we tested whether the efficacy of M. brunneum can be increased through a combination with CO2, emitted by Saccharomyces cerevisiae, as an attractant (=attract-and-kill). We aimed at a technical scale production of a formulated biological control agent offering a practical and economically feasible application for wireworm control. Therefore, a novel technical formulation process for encapsulated S. cerevisiae (Attract beads) and M. brunneum (Kill beads) was investigated. For the bead production by jet cutting, the parameters nozzle diameter, pump speed, cutting device speed and collecting distance were evaluated. In order to dry the beads in a short time while maintaining a high cell viability, different drying temperatures during fluidised-bed drying were tested and the best results were obtained with an inlet air temperature profile between 50°C and 40°C. CO2 production of the beads in the soil was highest for co-applied Attract and Kill beads. The potential of beads to modify wireworm behaviour (Agriotes sputator) was tested in a rhizotron experiment. The Attract-and-Kill treatment (co-applied beads) significantly attracted wireworms, whereas Attract beads and Kill beads alone showed a weak, but non-significant attraction. Wireworm mortality could not be enhanced due to a low rate of mycosis from M. brunneum infection.
Stichworte
Metarhizium brunneum; Saccharomyces cerevisiae; CO2; alginate beads; encapsulation; fluidised-bed drying
Erscheinungsjahr
2017
Zeitschriftentitel
Biocontrol Science and Technology
Band
27
Ausgabe
9
Seite(n)
1049-1070
ISSN
0958-3157
eISSN
1360-0478
Page URI
https://pub.uni-bielefeld.de/record/2931943

Zitieren

Humbert P, Przyklenk M, Vemmer M, Schumann M, Vidal S, Patel A. Technical scale production of encapsulated Saccharomyces cerevisiae and Metarhizium brunneum attractive to wireworms. Biocontrol Science and Technology. 2017;27(9):1049-1070.
Humbert, P., Przyklenk, M., Vemmer, M., Schumann, M., Vidal, S., & Patel, A. (2017). Technical scale production of encapsulated Saccharomyces cerevisiae and Metarhizium brunneum attractive to wireworms. Biocontrol Science and Technology, 27(9), 1049-1070. doi:10.1080/09583157.2017.1380164
Humbert, P., Przyklenk, M., Vemmer, M., Schumann, M., Vidal, S., and Patel, A. (2017). Technical scale production of encapsulated Saccharomyces cerevisiae and Metarhizium brunneum attractive to wireworms. Biocontrol Science and Technology 27, 1049-1070.
Humbert, P., et al., 2017. Technical scale production of encapsulated Saccharomyces cerevisiae and Metarhizium brunneum attractive to wireworms. Biocontrol Science and Technology, 27(9), p 1049-1070.
P. Humbert, et al., “Technical scale production of encapsulated Saccharomyces cerevisiae and Metarhizium brunneum attractive to wireworms”, Biocontrol Science and Technology, vol. 27, 2017, pp. 1049-1070.
Humbert, P., Przyklenk, M., Vemmer, M., Schumann, M., Vidal, S., Patel, A.: Technical scale production of encapsulated Saccharomyces cerevisiae and Metarhizium brunneum attractive to wireworms. Biocontrol Science and Technology. 27, 1049-1070 (2017).
Humbert, Pascal, Przyklenk, Michael, Vemmer, Marina, Schumann, Mario, Vidal, Stefan, and Patel, Anant. “Technical scale production of encapsulated Saccharomyces cerevisiae and Metarhizium brunneum attractive to wireworms”. Biocontrol Science and Technology 27.9 (2017): 1049-1070.
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