Calcium gluconate as cross-linker improves survival and shelf life of encapsulated and dried Metarhizium brunneum and Saccharomyces cerevisiae for the application as biological control agents

Humbert P, Przyklenk M, Vemmer M, Patel A (2017)
Journal of Microencapsulation 34(1): 47-56.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Humbert, Pascal; Przyklenk, Michael; Vemmer, Marina; Patel, AnantUniBi
Abstract / Bemerkung
Calcium chloride (CC) is the most common cross-linker for the encapsulation of biocontrol microorganisms in alginate beads. The aim of this study was to evaluate if calcium gluconate (CG) can replace CC as cross-linker and at the same time improve viability after drying and rehydration, hygroscopic properties, shelf life and nutrient supply. Hence, the biocontrol fungi Metarhizium brunneum and Saccharomyces cerevisiae were encapsulated in Ca-alginate beads supplemented with starch. Beads were dried and maximum survival was found in beads cross-linked with CG. Beads prepared with CG showed lower hygroscopic properties, but a higher shelf life for encapsulated fungi. Moreover, we demonstrated that gluconate has a nutritive effect on encapsulated fungi, leading to increased mycelium growth of M. brunneum and to enhanced CO2 release from beads containing Saccharomyces cerevisiae. The application of CG as crosslinker will pave the way towards increasing drying survival and shelf life of various, especially drying-sensitive microbes.
Stichworte
alginate bead; cross-linking; cell encapsulation; drying; entomopathogenic fungi; baker's yeast
Erscheinungsjahr
2017
Zeitschriftentitel
Journal of Microencapsulation
Band
34
Ausgabe
1
Seite(n)
47-56
ISSN
0265-2048
eISSN
1464-5246
Page URI
https://pub.uni-bielefeld.de/record/2931908

Zitieren

Humbert P, Przyklenk M, Vemmer M, Patel A. Calcium gluconate as cross-linker improves survival and shelf life of encapsulated and dried Metarhizium brunneum and Saccharomyces cerevisiae for the application as biological control agents. Journal of Microencapsulation. 2017;34(1):47-56.
Humbert, P., Przyklenk, M., Vemmer, M., & Patel, A. (2017). Calcium gluconate as cross-linker improves survival and shelf life of encapsulated and dried Metarhizium brunneum and Saccharomyces cerevisiae for the application as biological control agents. Journal of Microencapsulation, 34(1), 47-56. doi:10.1080/02652048.2017.1282550
Humbert, P., Przyklenk, M., Vemmer, M., and Patel, A. (2017). Calcium gluconate as cross-linker improves survival and shelf life of encapsulated and dried Metarhizium brunneum and Saccharomyces cerevisiae for the application as biological control agents. Journal of Microencapsulation 34, 47-56.
Humbert, P., et al., 2017. Calcium gluconate as cross-linker improves survival and shelf life of encapsulated and dried Metarhizium brunneum and Saccharomyces cerevisiae for the application as biological control agents. Journal of Microencapsulation, 34(1), p 47-56.
P. Humbert, et al., “Calcium gluconate as cross-linker improves survival and shelf life of encapsulated and dried Metarhizium brunneum and Saccharomyces cerevisiae for the application as biological control agents”, Journal of Microencapsulation, vol. 34, 2017, pp. 47-56.
Humbert, P., Przyklenk, M., Vemmer, M., Patel, A.: Calcium gluconate as cross-linker improves survival and shelf life of encapsulated and dried Metarhizium brunneum and Saccharomyces cerevisiae for the application as biological control agents. Journal of Microencapsulation. 34, 47-56 (2017).
Humbert, Pascal, Przyklenk, Michael, Vemmer, Marina, and Patel, Anant. “Calcium gluconate as cross-linker improves survival and shelf life of encapsulated and dried Metarhizium brunneum and Saccharomyces cerevisiae for the application as biological control agents”. Journal of Microencapsulation 34.1 (2017): 47-56.
Volltext(e)
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81 Humbert2017_Calcium gluconate as cross linker improves survival and shelf life of encapsulated and dried Metarhizium brunneum and Saccharomyces cerevisiae for.pdf 1.42 MB Request a Copy
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2019-09-06T07:37:02Z
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3 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Development of an attract-and-kill co-formulation containing Saccharomyces cerevisiae and neem extract attractive towards wireworms.
Humbert P, Vemmer M, Mävers F, Schumann M, Vidal S, Patel AV., Pest Manag Sci 74(7), 2018
PMID: 29281183
Endogenous arabitol and mannitol improve shelf life of encapsulated Metarhizium brunneum.
Krell V, Jakobs-Schoenwandt D, Persicke M, Patel AV., World J Microbiol Biotechnol 34(8), 2018
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Mayerhofer J, Eckard S, Hartmann M, Grabenweger G, Widmer F, Leuchtmann A, Enkerli J., FEMS Microbiol Ecol 93(10), 2017
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