Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor

Lohse R, Jakobs-Schönwandt D, Patel A (2014)
AMB Express 4(1): 47.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
A novel approach for biological control of insect pests could be the use of the endophytic entomopathogenic Beauveria bassiana isolate ATP-02. For the utilization of the endophyte as a commercial biocontrol agent, the fungus has to be mass-produced. B. bassiana was raised in shake flask cultures to produce high concentrations of total spores (TS), which include blastospores (BS) and submerged conidiospores (SCS). The highest concentration of 1.33×109 TS/mL and the highest yield of 5.32×1010 TS/g sucrose was obtained in the TKI broth with 5% sugar beet molasses which consists of 50% sucrose as a carbon source. In spite of the lower sugar concentration (2.5%) the amount of TS could be increased up to 11-times in contrast to the cultivation with 5% sucrose. The scale-up to a 2 L stirred tank reactor was carried out at 25°C, 200–600 rpm and 1 vvm at pH 5.5. A TS yield of 5.2×1010 TS/g sucrose corresponding to a SCS yield of 0.2×1010 SCS/g sucrose was obtained after 216 h. With regards to the culture medium the cost of 1012 TS amounts to 0.24 €. Plutella xylostella larvae, which were fed with oilseed rape leaves treated with spores from fermentation resulted in 77 ± 5% mortality. Moreover, spores from submerged cultivation were able to colonize oilseed rape leaves via leaf application. This is the first report of fermentation of an endophytic B. bassiana strain in a low-cost culture medium to very high yields of TS.
Erscheinungsjahr
Zeitschriftentitel
AMB Express
Band
4
Ausgabe
1
Art.-Nr.
47
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Lohse R, Jakobs-Schönwandt D, Patel A. Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor. AMB Express. 2014;4(1): 47.
Lohse, R., Jakobs-Schönwandt, D., & Patel, A. (2014). Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor. AMB Express, 4(1), 47. doi:10.1186/s13568-014-0047-6
Lohse, R., Jakobs-Schönwandt, D., and Patel, A. (2014). Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor. AMB Express 4:47.
Lohse, R., Jakobs-Schönwandt, D., & Patel, A., 2014. Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor. AMB Express, 4(1): 47.
R. Lohse, D. Jakobs-Schönwandt, and A. Patel, “Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor”, AMB Express, vol. 4, 2014, : 47.
Lohse, R., Jakobs-Schönwandt, D., Patel, A.: Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor. AMB Express. 4, : 47 (2014).
Lohse, Rieke, Jakobs-Schönwandt, Désirée, and Patel, Anant. “Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor”. AMB Express 4.1 (2014): 47.
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2018-10-31T16:10:34Z