Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy

Pilger C, Hachmeister H, Greife P, Weiß A, Wiebusch G, Huser T (2018)
Optics Express 26(22): 28312-28322.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Picosecond optical parametric oscillators (OPOs) with broad wavelength tunability are frequently used as light sources in hyperspectral coherent Raman scattering (CRS) microscopy. We investigate how changes in the pulse length during OPO wavelength tuning of the pump beam affect hyperspectral CRS imaging. We find that significant distortions of the resulting CRS spectra occur if the OPO is operated without monitoring pulse length variations. By utilizing a custom-written MATLAB based control program to counteract changes in pulse length, normalized and reproducible data sets can be acquired. We demonstrate this by comparing hyperspectral data obtained from pure substances, as well as relevant biological specimens.
Erscheinungsjahr
Zeitschriftentitel
Optics Express
Band
26
Ausgabe
22
Seite(n)
28312-28322
eISSN
Finanzierungs-Informationen
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
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Pilger C, Hachmeister H, Greife P, Weiß A, Wiebusch G, Huser T. Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy. Optics Express. 2018;26(22):28312-28322.
Pilger, C., Hachmeister, H., Greife, P., Weiß, A., Wiebusch, G., & Huser, T. (2018). Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy. Optics Express, 26(22), 28312-28322. doi:10.1364/oe.26.028312
Pilger, C., Hachmeister, H., Greife, P., Weiß, A., Wiebusch, G., and Huser, T. (2018). Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy. Optics Express 26, 28312-28322.
Pilger, C., et al., 2018. Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy. Optics Express, 26(22), p 28312-28322.
C. Pilger, et al., “Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy”, Optics Express, vol. 26, 2018, pp. 28312-28322.
Pilger, C., Hachmeister, H., Greife, P., Weiß, A., Wiebusch, G., Huser, T.: Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy. Optics Express. 26, 28312-28322 (2018).
Pilger, Christian, Hachmeister, Henning, Greife, Paul, Weiß, Alexa, Wiebusch, Gerd, and Huser, Thomas. “Pulse length variation causing spectral distortions in OPO-based hyperspectral coherent Raman scattering microscopy”. Optics Express 26.22 (2018): 28312-28322.
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2018-10-17T11:12:15Z

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