One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst

Mindt M, Risse JM, Gruß H, Sewald N, Eikmanns BJ, Wendisch VF (2018)
Scientific Reports 8: 12895.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
N-methylated amino acids are found in Nature in various biological compounds. N-methylation of amino acids has been shown to improve pharmacokinetic properties of peptide drugs due to conformational changes, improved proteolytic stability and/or higher lipophilicity. Due to these characteristics N-methylated amino acids received increasing interest by the pharmaceutical industry. Syntheses of N-methylated amino acids by chemical and biocatalytic approaches are known, but often show incomplete stereoselectivity, low yields or expensive co-factor regeneration. So far a one-step fermentative process from sugars has not yet been described. Here, a one-step conversion of sugars and methylamine to the N-methylated amino acid N-methyl-l-alanine was developed. A whole-cell biocatalyst was derived from a pyruvate overproducing C. glutamicum strain by heterologous expression of the N-methyl-l-amino acid dehydrogenase gene from Pseudomonas putida. As proof-of-concept, N-methyl-l-alanine titers of 31.7 g L−1 with a yield of 0.71 g per g glucose were achieved in fed-batch cultivation. The C. glutamicum strain producing this imine reductase enzyme was engineered further to extend this green chemistry route to production of N-methyl-l-alanine from alternative feed stocks such as starch or the lignocellulosic sugars xylose and arabinose.
Erscheinungsjahr
2018
Zeitschriftentitel
Scientific Reports
Band
8
Art.-Nr.
12895
eISSN
2045-2322
Finanzierungs-Informationen
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
Page URI
https://pub.uni-bielefeld.de/record/2930292

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Mindt M, Risse JM, Gruß H, Sewald N, Eikmanns BJ, Wendisch VF. One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst. Scientific Reports. 2018;8: 12895.
Mindt, M., Risse, J. M., Gruß, H., Sewald, N., Eikmanns, B. J., & Wendisch, V. F. (2018). One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst. Scientific Reports, 8, 12895. doi:10.1038/s41598-018-31309-5
Mindt, M., Risse, J. M., Gruß, H., Sewald, N., Eikmanns, B. J., and Wendisch, V. F. (2018). One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst. Scientific Reports 8:12895.
Mindt, M., et al., 2018. One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst. Scientific Reports, 8: 12895.
M. Mindt, et al., “One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst”, Scientific Reports, vol. 8, 2018, : 12895.
Mindt, M., Risse, J.M., Gruß, H., Sewald, N., Eikmanns, B.J., Wendisch, V.F.: One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst. Scientific Reports. 8, : 12895 (2018).
Mindt, Melanie, Risse, Joe Max, Gruß, Hendrik, Sewald, Norbert, Eikmanns, Bernhard J., and Wendisch, Volker F. “One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst”. Scientific Reports 8 (2018): 12895.
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2019-09-06T09:19:00Z
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