Design, characterisation and application of alginate-based encapsulated pig liver esterase

Pauly J, Gröger H, Patel A (2018)
Journal of Biotechnology 280: 42-48.

Zeitschriftenaufsatz | E-Veröff. vor dem Druck | Englisch
 
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Abstract / Bemerkung
Encapsulation of hydrolases in biopolymer-based hydrogels often suffers from low activities and encapsulation efficiencies along with high leaching and unsatisfactory recycling properties. Exemplified for the encapsulation of pig liver esterase the coating of alginate and chitosan beads have been studied by creating various biopolymer hydrogel beads. Enzyme activity and encapsulation efficiency were notably enhanced by chitosan coating of alginate beads while leaching remained nearly unchanged. This was caused by the enzymatic reaction acidifying the matrix, which increased enzyme retention through enhanced electrostatic enzyme-alginate interaction but decreased activity through enzyme deactivation. A practical and ready-to-use method for visualising pH in beads during reaction by co-encapsulation of a conventional pH indicator was also found. Our method proves that pH control inside the beads can only be realised by buffering. The resulting beads provided a specific activity of 0.267 μmol ∙ min-1 ∙ mg-1, effectiveness factor 0.88, encapsulation efficiency of 88%, 5% leaching and good recycling properties. This work will contribute towards better understanding and application of encapsulated hydrolases for enzymatic syntheses.
Stichworte
biocatalysis; immobilisation; encapsulation; esterase; biopolymer
Erscheinungsjahr
2018
Zeitschriftentitel
Journal of Biotechnology
Band
280
Seite(n)
42-48
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/2920724

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Pauly J, Gröger H, Patel A. Design, characterisation and application of alginate-based encapsulated pig liver esterase. Journal of Biotechnology. 2018;280:42-48.
Pauly, J., Gröger, H., & Patel, A. (2018). Design, characterisation and application of alginate-based encapsulated pig liver esterase. Journal of Biotechnology, 280, 42-48. doi:10.1016/j.jbiotec.2018.05.017
Pauly, J., Gröger, H., and Patel, A. (2018). Design, characterisation and application of alginate-based encapsulated pig liver esterase. Journal of Biotechnology 280, 42-48.
Pauly, J., Gröger, H., & Patel, A., 2018. Design, characterisation and application of alginate-based encapsulated pig liver esterase. Journal of Biotechnology, 280, p 42-48.
J. Pauly, H. Gröger, and A. Patel, “Design, characterisation and application of alginate-based encapsulated pig liver esterase”, Journal of Biotechnology, vol. 280, 2018, pp. 42-48.
Pauly, J., Gröger, H., Patel, A.: Design, characterisation and application of alginate-based encapsulated pig liver esterase. Journal of Biotechnology. 280, 42-48 (2018).
Pauly, Jan, Gröger, Harald, and Patel, Anant. “Design, characterisation and application of alginate-based encapsulated pig liver esterase”. Journal of Biotechnology 280 (2018): 42-48.
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