Development of a tomato plant resistant to Clavibacter michiganensis using the endolysin gene of bacteriophage CMP1 as a transgene

Wittmann J, Brancato C, Berendzen KW, Dreiseikelmann B (2016)
PLANT PATHOLOGY 65(3): 496-502.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Wittmann, J.; Brancato, C.; Berendzen, K. W.; Dreiseikelmann, BrigitteUniBi
Abstract / Bemerkung
The bacteriophage CMP1 endolysin gene (lys), encoding, a peptidase that was shown to effectively reduce Clavibacter michiganensis by specifically hydrolysing its murein, was transferred into tomato plants by Agrobacterium-mediated transformation. The presence of the gene was verified by PCR and the gene product was confirmed in immunoblots and stably expressed over three generations. Transgenic tomato plants did not show disease symptoms after infection with C.michiganensis subsp. michiganensis, despite the fact that small amounts of bacteria could still be identified in xylem sap and leaf extracts, although in significantly reduced amounts.
Stichworte
bacteriophage; Clavibacter michiganensis; endolysin; transgenic tomato; plant
Erscheinungsjahr
2016
Zeitschriftentitel
PLANT PATHOLOGY
Band
65
Ausgabe
3
Seite(n)
496-502
ISSN
0032-0862
eISSN
1365-3059
Page URI
https://pub.uni-bielefeld.de/record/2917140

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Wittmann J, Brancato C, Berendzen KW, Dreiseikelmann B. Development of a tomato plant resistant to Clavibacter michiganensis using the endolysin gene of bacteriophage CMP1 as a transgene. PLANT PATHOLOGY. 2016;65(3):496-502.
Wittmann, J., Brancato, C., Berendzen, K. W., & Dreiseikelmann, B. (2016). Development of a tomato plant resistant to Clavibacter michiganensis using the endolysin gene of bacteriophage CMP1 as a transgene. PLANT PATHOLOGY, 65(3), 496-502. doi:10.1111/ppa.12417
Wittmann, J., Brancato, C., Berendzen, K. W., and Dreiseikelmann, B. (2016). Development of a tomato plant resistant to Clavibacter michiganensis using the endolysin gene of bacteriophage CMP1 as a transgene. PLANT PATHOLOGY 65, 496-502.
Wittmann, J., et al., 2016. Development of a tomato plant resistant to Clavibacter michiganensis using the endolysin gene of bacteriophage CMP1 as a transgene. PLANT PATHOLOGY, 65(3), p 496-502.
J. Wittmann, et al., “Development of a tomato plant resistant to Clavibacter michiganensis using the endolysin gene of bacteriophage CMP1 as a transgene”, PLANT PATHOLOGY, vol. 65, 2016, pp. 496-502.
Wittmann, J., Brancato, C., Berendzen, K.W., Dreiseikelmann, B.: Development of a tomato plant resistant to Clavibacter michiganensis using the endolysin gene of bacteriophage CMP1 as a transgene. PLANT PATHOLOGY. 65, 496-502 (2016).
Wittmann, J., Brancato, C., Berendzen, K. W., and Dreiseikelmann, Brigitte. “Development of a tomato plant resistant to Clavibacter michiganensis using the endolysin gene of bacteriophage CMP1 as a transgene”. PLANT PATHOLOGY 65.3 (2016): 496-502.