Dynamic AFM force spectroscopy of DNA using FM mode with constant excitation

Horstmeier S, Walhorn V, Anselmetti D (2017)
EPL 117(3): 38005.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
We report single-molecule force spectroscopy experiments on double-stranded DNA using a dynamically oscillating AFM cantilever driven in a frequency modulation mode with a constant excitation (CE) amplitude. While the DNA molecule is stretched, we measured the shift of the cantilever amplitude and the resonance frequency and estimated both the conservative forces and the dissipated energy. Typical elastic features of the calculated force distance curves are in nice agreement with data known from quasi-static stretching experiments. Analysis of the data indicates that the melting dynamics of the DNA base pairs depends on the amount of already melted base pairs and the base pair recombination rate. Furthermore, we observe a reduction of the molecular elasticity modulus, which is most likely caused by the cantilever dynamics leading to a destabilization of stacking and hydrogen bond interactions. editor's choice Copyright (C) EPLA, 2017
Erscheinungsjahr
2017
Zeitschriftentitel
EPL
Band
117
Ausgabe
3
Art.-Nr.
38005
ISSN
0295-5075
eISSN
1286-4854
Page URI
https://pub.uni-bielefeld.de/record/2916570

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Horstmeier S, Walhorn V, Anselmetti D. Dynamic AFM force spectroscopy of DNA using FM mode with constant excitation. EPL. 2017;117(3): 38005.
Horstmeier, S., Walhorn, V., & Anselmetti, D. (2017). Dynamic AFM force spectroscopy of DNA using FM mode with constant excitation. EPL, 117(3), 38005. doi:10.1209/0295-5075/117/38005
Horstmeier, Sebastian, Walhorn, Volker, and Anselmetti, Dario. 2017. “Dynamic AFM force spectroscopy of DNA using FM mode with constant excitation”. EPL 117 (3): 38005.
Horstmeier, S., Walhorn, V., and Anselmetti, D. (2017). Dynamic AFM force spectroscopy of DNA using FM mode with constant excitation. EPL 117:38005.
Horstmeier, S., Walhorn, V., & Anselmetti, D., 2017. Dynamic AFM force spectroscopy of DNA using FM mode with constant excitation. EPL, 117(3): 38005.
S. Horstmeier, V. Walhorn, and D. Anselmetti, “Dynamic AFM force spectroscopy of DNA using FM mode with constant excitation”, EPL, vol. 117, 2017, : 38005.
Horstmeier, S., Walhorn, V., Anselmetti, D.: Dynamic AFM force spectroscopy of DNA using FM mode with constant excitation. EPL. 117, : 38005 (2017).
Horstmeier, Sebastian, Walhorn, Volker, and Anselmetti, Dario. “Dynamic AFM force spectroscopy of DNA using FM mode with constant excitation”. EPL 117.3 (2017): 38005.
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