The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens

Hedtmann C, Guo W, Reifschneider E, Heiber I, Hiltscher H, van Buer J, Barsch A, Niehaus K, Rowan B, Lortzing T, Steppuhn A, et al. (2017)

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Hedtmann, Christiane; Guo, Wei; Reifschneider, Elena; Heiber, Isabelle; Hiltscher, Heiko; van Buer, Joern; Barsch, Aiko; Niehaus, KarstenUniBi; Rowan, Beth; Lortzing, Tobias; Steppuhn, Anke; Baier, Margarete
Abstract / Bemerkung
The redox imbalanced 6 mutant (rimb6) of Arabidopsis thaliana was isolated in a genetic screening approach for mutants with defects in chloroplast-to-nucleus redox signaling. It has an atypically low activation status of the 2-Cys peroxiredoxin-A promoter in the seedling stage. rimb6 shows wildtype-like germination, seedling development and greening, but slower growth and reduced biomass in the rosette stage. Mapping of the casual mutation revealed that rimb6 carries a single nucleotide polymorphism in the gene encoding CONSTITUTIVE EXPRESSER OF PATHOGENESIS RELATED (PR) GENES 1, CPR1 (At4g12560), leading to a premature stop codon. CPR1 is known as a repressor of pathogen signaling and regulator of microtubule organization. Allelism of rimb6 and cprl revealed a function of CPR1 in chloroplast stress protection. Expression studies in pathogen signaling mutants demonstrated that CPR1-mediated activation of genes for photosynthesis and chloroplast antioxidant protection is, in contrast to activation of pathogen responses, regulated independently from PAD4-controlled salicylic acid (SA) accumulation. We conclude that the support of plastid function is a basic, SA-independent function of CPR1.
Arabidopsis; chloroplast; CPR1; reactive oxygen species; redox; imbalanced mutant; signaling
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Hedtmann C, Guo W, Reifschneider E, et al. The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens. FRONTIERS IN PLANT SCIENCE. 2017;8: 1650.
Hedtmann, C., Guo, W., Reifschneider, E., Heiber, I., Hiltscher, H., van Buer, J., Barsch, A., et al. (2017). The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens. FRONTIERS IN PLANT SCIENCE, 8, 1650. doi:10.3389/fpls.2017.01650
Hedtmann, C., Guo, W., Reifschneider, E., Heiber, I., Hiltscher, H., van Buer, J., Barsch, A., Niehaus, K., Rowan, B., Lortzing, T., et al. (2017). The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens. FRONTIERS IN PLANT SCIENCE 8:1650.
Hedtmann, C., et al., 2017. The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens. FRONTIERS IN PLANT SCIENCE, 8: 1650.
C. Hedtmann, et al., “The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens”, FRONTIERS IN PLANT SCIENCE, vol. 8, 2017, : 1650.
Hedtmann, C., Guo, W., Reifschneider, E., Heiber, I., Hiltscher, H., van Buer, J., Barsch, A., Niehaus, K., Rowan, B., Lortzing, T., Steppuhn, A., Baier, M.: The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens. FRONTIERS IN PLANT SCIENCE. 8, : 1650 (2017).
Hedtmann, Christiane, Guo, Wei, Reifschneider, Elena, Heiber, Isabelle, Hiltscher, Heiko, van Buer, Joern, Barsch, Aiko, Niehaus, Karsten, Rowan, Beth, Lortzing, Tobias, Steppuhn, Anke, and Baier, Margarete. “The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens”. FRONTIERS IN PLANT SCIENCE 8 (2017): 1650.

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Daten bereitgestellt von Europe PubMed Central.

Preparing plants for improved cold tolerance by priming.
Baier M, Bittner A, Prescher A, van Buer J., Plant Cell Environ 42(3), 2019
PMID: 29974962
Mapping of a Major QTL for Ceratocystis Wilt Disease in an F1 Population of Theobroma cacao.
Fernandes LDS, Royaert S, Corrêa FM, Mustiga GM, Marelli JP, Corrêa RX, Motamayor JC., Front Plant Sci 9(), 2018
PMID: 29491879
The role of chloroplasts in plant pathology.
Sowden RG, Watson SJ, Jarvis P., Essays Biochem 62(1), 2018
PMID: 29273582

95 References

Daten bereitgestellt von Europe PubMed Central.

Genome-wide insertional mutagenesis of Arabidopsis thaliana.
Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR., Science 301(5633), 2003
PMID: 12893945
QuantPrime--a flexible tool for reliable high-throughput primer design for quantitative PCR.
Arvidsson S, Kwasniewski M, Riano-Pachon DM, Mueller-Roeber B., BMC Bioinformatics 9(), 2008
PMID: 18976492
The water-water cycle as alternative photon and electron sinks.
Asada K., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 355(1402), 2000
PMID: 11127996
Jasmonate-induced lipid peroxidation in barley leaves initiated by distinct 13-LOX forms of chloroplasts.
Bachmann A, Hause B, Maucher H, Garbe E, Voros K, Weichert H, Wasternack C, Feussner I., Biol. Chem. 383(10), 2002
PMID: 12452441
The costs and benefits of oxygen for photosynthesizing plant cells
Baier M., Dietz K.-J.., 1999
Reguation of genes encoding chloroplast antioxidant enzymes in comparison to regulation of the extra-plastidic antioxidant defense system
Baier M., Pitsch N., Mellenthin M., Guo W.., 2010
Control of ascorbate synthesis by respiration and its implications for stress responses.
Millar AH, Mittova V, Kiddle G, Heazlewood JL, Bartoli CG, Theodoulou FL, Foyer CH., Plant Physiol. 133(2), 2003
PMID: 14555771
Stability of plant immune-receptor resistance proteins is controlled by SKP1-CULLIN1-F-box (SCF)-mediated protein degradation.
Cheng YT, Li Y, Huang S, Huang Y, Dong X, Zhang Y, Li X., Proc. Natl. Acad. Sci. U.S.A. 108(35), 2011
PMID: 21873230
Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis.
Czechowski T, Stitt M, Altmann T, Udvardi MK, Scheible WR., Plant Physiol. 139(1), 2005
PMID: 16166256
Peroxisomes as a source of superoxide and hydrogen peroxide in stressed plants.
del Rio LA, Palma JM, Sandalio LM, Corpas FJ, Pastori GM, Bueno P, Lopez-Huertas E., Biochem. Soc. Trans. 24(2), 1996
PMID: 8736778
Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana.
Du YY, Wang PC, Chen J, Song CP., J Integr Plant Biol 50(10), 2008
PMID: 19017119
Systemic acquired resistance.
Durrant WE, Dong X., Annu Rev Phytopathol 42(), 2004
PMID: 15283665
Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4.
Feys BJ, Moisan LJ, Newman MA, Parker JE., EMBO J. 20(19), 2001
PMID: 11574472
Photooxidative stress in plants
Foyer C., Lelandais M., Kunert K.., 1994
Nutrient sensing in plant meristems.
Francis D, Halford NG., Plant Mol. Biol. 60(6), 2006
PMID: 16724265
Transcriptomic footprints disclose specificity of reactive oxygen species signaling in Arabidopsis.
Gadjev I, Vanderauwera S, Gechev TS, Laloi C, Minkov IN, Shulaev V, Apel K, Inze D, Mittler R, Van Breusegem F., Plant Physiol. 141(2), 2006
PMID: 16603662
Isochorismate synthase 1 is required for thylakoid organization, optimal plastoquinone redox status, and state transitions in Arabidopsis thaliana.
Gawronski P, Gorecka M, Bederska M, Rusaczonek A, Slesak I, Kruk J, Karpinski S., J. Exp. Bot. 64(12), 2013
PMID: 23956412
The F-box protein CPR1/CPR30 negatively regulates R protein SNC1 accumulation.
Gou M, Shi Z, Zhu Y, Bao Z, Wang G, Hua J., Plant J. 69(3), 2011
PMID: 21967323
An F-box gene, CPR30, functions as a negative regulator of the defense response in Arabidopsis.
Gou M, Su N, Zheng J, Huai J, Wu G, Zhao J, He J, Tang D, Yang S, Wang G., Plant J. 60(5), 2009
PMID: 19682297
Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis.
Greene EA, Codomo CA, Taylor NE, Henikoff JG, Till BJ, Reynolds SH, Enns LC, Burtner C, Johnson JE, Odden AR, Comai L, Henikoff S., Genetics 164(2), 2003
PMID: 12807792
Regulation of basal and oxidative stress-triggered jasmonic acid-related gene expression by glutathione.
Han Y, Mhamdi A, Chaouch S, Noctor G., Plant Cell Environ. 36(6), 2013
PMID: 23210597
The redox imbalanced mutants of Arabidopsis differentiate signaling pathways for redox regulation of chloroplast antioxidant enzymes.
Heiber I, Stroher E, Raatz B, Busse I, Kahmann U, Bevan MW, Dietz KJ, Baier M., Plant Physiol. 143(4), 2007
PMID: 17337533
The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymes.
Hiltscher H, Rudnik R, Shaikhali J, Heiber I, Mellenthin M, Meirelles Duarte I, Schuster G, Kahmann U, Baier M., Front Plant Sci 5(), 2014
PMID: 25295044
NTRC and chloroplast-generated reactive oxygen species regulate Pseudomonas syringae pv. tomato disease development in tomato and Arabidopsis.
Ishiga Y, Ishiga T, Wangdi T, Mysore KS, Uppalapati SR., Mol. Plant Microbe Interact. 25(3), 2012
PMID: 22112219
Arabidopsis map-based cloning in the post-genome era.
Jander G, Norris SR, Rounsley SD, Bush DF, Levin IM, Last RL., Plant Physiol. 129(2), 2002
PMID: 12068090
Arabidopsis thaliana PAD4 encodes a lipase-like gene that is important for salicylic acid signaling.
Jirage D, Tootle TL, Reuber TL, Frost LN, Feys BJ, Parker JE, Ausubel FM, Glazebrook J., Proc. Natl. Acad. Sci. U.S.A. 96(23), 1999
PMID: 10557364
Constitutive salicylic acid-dependent signaling in cpr1 and cpr6 mutants requires PAD4.
Jirage D, Zhou N, Cooper B, Clarke JD, Dong X, Glazebrook J., Plant J. 26(4), 2001
PMID: 11439127
The plant immune system.
Jones JD, Dangl JL., Nature 444(7117), 2006
PMID: 17108957
Signalling and cell death in ozone-exposed plants
Kangasjärvi J., Jaspers P., Kollist H.., 2005
Diverse roles for chloroplast stromal and thylakoid-bound ascorbate peroxidases in plant stress responses.
Kangasjarvi S, Lepisto A, Hannikainen K, Piippo M, Luomala EM, Aro EM, Rintamaki E., Biochem. J. 412(2), 2008
PMID: 18318659
The plant-specific function of 2-Cys peroxiredoxin-mediated detoxification of peroxides in the redox-hierarchy of photosynthetic electron flux.
Konig J, Baier M, Horling F, Kahmann U, Harris G, Schurmann P, Dietz KJ., Proc. Natl. Acad. Sci. U.S.A. 99(8), 2002
PMID: 11929977
GMD@CSB.DB: the Golm Metabolome Database.
Kopka J, Schauer N, Krueger S, Birkemeyer C, Usadel B, Bergmuller E, Dormann P, Weckwerth W, Gibon Y, Stitt M, Willmitzer L, Fernie AR, Steinhauser D., Bioinformatics 21(8), 2004
PMID: 15613389
Photosynthetic alterations of pea leaves infected systemically by pea enation mosaic virus: A coordinated decrease in efficiencies of CO(2) assimilation and photosystem II photochemistry.
Kyselakova H, Prokopova J, Naus J, Novak O, Navratil M, Safarova D, Spundova M, Ilik P., Plant Physiol. Biochem. 49(11), 2011
PMID: 22000051
Analysis of salicylic acid-dependent pathways in Arabidopsis thaliana following infection with Plasmodiophora brassicae and the influence of salicylic acid on disease.
Lovelock DA, Sola I, Marschollek S, Donald CE, Rusak G, van Pee KH, Ludwig-Muller J, Cahill DM., Mol. Plant Pathol. 17(8), 2016
PMID: 26719902
H2O2-triggered retrograde signaling from chloroplasts to nucleus plays specific role in response to stress.
Maruta T, Noshi M, Tanouchi A, Tamoi M, Yabuta Y, Yoshimura K, Ishikawa T, Shigeoka S., J. Biol. Chem. 287(15), 2012
PMID: 22334687
The plant NADPH oxidase RBOHD mediates rapid systemic signaling in response to diverse stimuli.
Miller G, Schlauch K, Tam R, Cortes D, Torres MA, Shulaev V, Dangl JL, Mittler R., Sci Signal 2(84), 2009
PMID: 19690331
Gain- and loss-of-function mutations in Zat10 enhance the tolerance of plants to abiotic stress.
Mittler R, Kim Y, Song L, Coutu J, Coutu A, Ciftci-Yilmaz S, Lee H, Stevenson B, Zhu JK., FEBS Lett. 580(28-29), 2006
PMID: 17112521
Chloroplast signaling and LESION SIMULATING DISEASE1 regulate crosstalk between light acclimation and immunity in Arabidopsis.
Muhlenbock P, Szechynska-Hebda M, Plaszczyca M, Baudo M, Mateo A, Mullineaux PM, Parker JE, Karpinska B, Karpinski S., Plant Cell 20(9), 2008
PMID: 18790826
Are diverse signalling pathways integrated in the regulation of arabidopsis antioxidant defence gene expression in response to excess excitation energy?
Mullineaux P, Ball L, Escobar C, Karpinska B, Creissen G, Karpinski S., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 355(1402), 2000
PMID: 11128006
Plastid-to-nucleus retrograde signaling.
Nott A, Jung HS, Koussevitzky S, Chory J., Annu Rev Plant Biol 57(), 2006
PMID: 16669780
Rapid induction of distinct stress responses after the release of singlet oxygen in Arabidopsis.
op den Camp RG, Przybyla D, Ochsenbein C, Laloi C, Kim C, Danon A, Wagner D, Hideg E, Gobel C, Feussner I, Nater M, Apel K., Plant Cell 15(10), 2003
PMID: 14508004
Sequencing of natural strains of Arabidopsis thaliana with short reads.
Ossowski S, Schneeberger K, Clark RM, Lanz C, Warthmann N, Weigel D., Genome Res. 18(12), 2008
PMID: 18818371
Chloroplast redox signals: how photosynthesis controls its own genes.
Pfannschmidt T., Trends Plant Sci. 8(1), 2003
PMID: 12523998
Photosynthetic responses of lettuce to downy mildew infection and cytokinin treatment.
Prokopova J, Spundova M, Sedlarova M, Husickova A, Novotny R, Dolezal K, Naus J, Lebeda A., Plant Physiol. Biochem. 48(8), 2010
PMID: 20471849
Salicylic acid beyond defence: its role in plant growth and development.
Rivas-San Vicente M, Plasencia J., J. Exp. Bot. 62(10), 2011
PMID: 21357767
Systemic and intracellular responses to photooxidative stress in Arabidopsis.
Rossel JB, Wilson PB, Hussain D, Woo NS, Gordon MJ, Mewett OP, Howell KA, Whelan J, Kazan K, Pogson BJ., Plant Cell 19(12), 2007
PMID: 18156220
Cell proliferation and hair tip growth in the Arabidopsis root are under mechanistically different forms of redox control.
Sanchez-Fernandez R, Fricker M, Corben LB, White NS, Sheard N, Leaver CJ, Van Montagu M , Inze D, May MJ., Proc. Natl. Acad. Sci. U.S.A. 94(6), 1997
PMID: 11038608
DNA sequencing with chain-terminating inhibitors.
Sanger F, Nicklen S, Coulson AR., Proc. Natl. Acad. Sci. U.S.A. 74(12), 1977
PMID: 271968
Redox regulation in shoot growth, SAM maintenance and flowering.
Schippers JH, Foyer CH, van Dongen JT., Curr. Opin. Plant Biol. 29(), 2016
PMID: 26799134
Simultaneous alignment of short reads against multiple genomes.
Schneeberger K, Hagmann J, Ossowski S, Warthmann N, Gesing S, Kohlbacher O, Weigel D., Genome Biol. 10(9), 2009
PMID: 19761611
SHOREmap: simultaneous mapping and mutation identification by deep sequencing.
Schneeberger K, Ossowski S, Lanz C, Juul T, Petersen AH, Nielsen KL, Jorgensen JE, Weigel D, Andersen SU., Nat. Methods 6(8), 2009
PMID: 19644454
The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes.
Shaikhali J, Heiber I, Seidel T, Stroher E, Hiltscher H, Birkmann S, Dietz KJ, Baier M., BMC Plant Biol. 8(), 2008
PMID: 18439303
NPR1 modulates cross-talk between salicylate- and jasmonate-dependent defense pathways through a novel function in the cytosol.
Spoel SH, Koornneef A, Claessens SM, Korzelius JP, Van Pelt JA, Mueller MJ, Buchala AJ, Metraux JP, Brown R, Kazan K, Van Loon LC, Dong X, Pieterse CM., Plant Cell 15(3), 2003
PMID: 12615947
Chloroplast to nucleus communication triggered by accumulation of Mg-protoporphyrinIX.
Strand A, Asami T, Alonso J, Ecker JR, Chory J., Nature 421(6918), 2003
PMID: 12511958
Salicylic acid antagonism of EDS1-driven cell death is important for immune and oxidative stress responses in Arabidopsis.
Straus MR, Rietz S, Ver Loren van Themaat E, Bartsch M, Parker JE., Plant J. 62(4), 2010
PMID: 20163553
Evidence for light wavelength-specific photoelectrophysiological signaling and memory of excess light episodes in Arabidopsis.
Szechynska-Hebda M, Kruk J, Gorecka M, Karpinska B, Karpinski S., Plant Cell 22(7), 2010
PMID: 20639446

Vranova E., Van F., Dat J., Belles-Boix E., Inzé D.., 2002
Proteomic analysis of Arabidopsis constitutive expresser of pathogenesis related gene 1 (cpr30/cpr1-2) mutant
Wang X., Guo M., Bu H., Zhang S., Wang G.., 2014
Isochorismate synthase is required to synthesize salicylic acid for plant defence.
Wildermuth MC, Dewdney J, Wu G, Ausubel FM., Nature 414(6863), 2001
PMID: 11734859
Abscisic acid represses the transcription of chloroplast genes.
Yamburenko MV, Zubo YO, Vankova R, Kusnetsov VV, Kulaeva ON, Borner T., J. Exp. Bot. 64(14), 2013
PMID: 24078671
The diurnal metabolism of leaf starch.
Zeeman SC, Smith SM, Smith AM., Biochem. J. 401(1), 2007
PMID: 17150041
PAD4 functions upstream from salicylic acid to control defense responses in Arabidopsis.
Zhou N, Tootle TL, Tsui F, Klessig DF, Glazebrook J., Plant Cell 10(6), 1998
PMID: 9634589
Host and non-host pathogens elicit different jasmonate/ethylene responses in Arabidopsis.
Zimmerli L, Stein M, Lipka V, Schulze-Lefert P, Somerville S., Plant J. 40(5), 2004
PMID: 15546348


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