The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism

Rosar C, Kanonenberg K, Nanda AM, Mielewczik M, Bräutigam A, Novak O, Strnad M, Walter A, Weber APM (2012)
Molecular Plant 5(6): 1227-1241.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
OA 3.17 MB
Rosar, Christian; Kanonenberg, Kerstin; Nanda, Arun M.; Mielewczik, Michael; Bräutigam, AndreaUniBi ; Novak, Ondrej; Strnad, Miroslav; Walter, Achim; Weber, Andreas P. M.
Abstract / Bemerkung
A series of reticulated Arabidopsis thaliana mutants were previously described. All mutants show a reticulate leaf pattern, namely green veins on a pale leaf lamina. They have an aberrant mesophyll structure but an intact layer of bundle sheath cells around the veins. Here, we unravel the function of the previously described reticulated EMS-mutant dov1 (differential development of vascular associated cells 1). By positional cloning, we identified the mutated gene, which encodes glutamine phosphoribosyl pyrophosphate aminotransferase 2 (ATase2), an enzyme catalyzing the first step of purine nucleotide biosynthesis. dov1 is allelic to the previously characterized cia1-2 mutant that was isolated in a screen for mutants with impaired chloroplast protein import. We show that purine-derived total cytokinins are lowered in dov1 and crosses with phytohormone reporter lines revealed differential reporter activity patterns in dov1. Metabolite profiling unraveled that amino acids that are involved in purine biosynthesis are increased in dov1. This study identified the molecular basis of an established mutant line, which has the potential for further investigation of the interaction between metabolism and leaf development.
leaf development; reticulated mutants; cytokinin; growth kinetics; purine metabolism
Molecular Plant
Page URI


Rosar C, Kanonenberg K, Nanda AM, et al. The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism. Molecular Plant. 2012;5(6):1227-1241.
Rosar, C., Kanonenberg, K., Nanda, A. M., Mielewczik, M., Bräutigam, A., Novak, O., Strnad, M., et al. (2012). The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism. Molecular Plant, 5(6), 1227-1241. doi:10.1093/mp/sss045
Rosar, Christian, Kanonenberg, Kerstin, Nanda, Arun M., Mielewczik, Michael, Bräutigam, Andrea, Novak, Ondrej, Strnad, Miroslav, Walter, Achim, and Weber, Andreas P. M. 2012. “The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism”. Molecular Plant 5 (6): 1227-1241.
Rosar, C., Kanonenberg, K., Nanda, A. M., Mielewczik, M., Bräutigam, A., Novak, O., Strnad, M., Walter, A., and Weber, A. P. M. (2012). The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism. Molecular Plant 5, 1227-1241.
Rosar, C., et al., 2012. The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism. Molecular Plant, 5(6), p 1227-1241.
C. Rosar, et al., “The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism”, Molecular Plant, vol. 5, 2012, pp. 1227-1241.
Rosar, C., Kanonenberg, K., Nanda, A.M., Mielewczik, M., Bräutigam, A., Novak, O., Strnad, M., Walter, A., Weber, A.P.M.: The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism. Molecular Plant. 5, 1227-1241 (2012).
Rosar, Christian, Kanonenberg, Kerstin, Nanda, Arun M., Mielewczik, Michael, Bräutigam, Andrea, Novak, Ondrej, Strnad, Miroslav, Walter, Achim, and Weber, Andreas P. M. “The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism”. Molecular Plant 5.6 (2012): 1227-1241.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
Dieses Objekt ist durch das Urheberrecht und/oder verwandte Schutzrechte geschützt. [...]
Access Level
OA Open Access
Zuletzt Hochgeladen
MD5 Prüfsumme

9 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Uncovering C4-like photosynthesis in C3 vascular cells.
Gao Z, Shen W, Chen G., J Exp Bot 69(15), 2018
PMID: 29684188
Mutations in the Arabidopsis AtMRS2-11/AtMGT10/VAR5 Gene Cause Leaf Reticulation.
Liang S, Qi Y, Zhao J, Li Y, Wang R, Shao J, Liu X, An L, Yu F., Front Plant Sci 8(), 2017
PMID: 29234332
Plastid signals and the bundle sheath: mesophyll development in reticulate mutants.
Lundquist PK, Rosar C, Bräutigam A, Weber AP., Mol Plant 7(1), 2014
PMID: 24046062
Influence of plastids on light signalling and development.
Larkin RM., Philos Trans R Soc Lond B Biol Sci 369(1640), 2014
PMID: 24591718
Reticulate leaves and stunted roots are independent phenotypes pointing at opposite roles of the phosphoenolpyruvate/phosphate translocator defective in cue1 in the plastids of both organs.
Staehr P, Löttgert T, Christmann A, Krueger S, Rosar C, Rolčík J, Novák O, Strnad M, Bell K, Weber AP, Flügge UI, Häusler RE., Front Plant Sci 5(), 2014
PMID: 24782872
How do 'housekeeping' genes control organogenesis?--Unexpected new findings on the role of housekeeping genes in cell and organ differentiation.
Tsukaya H, Byrne ME, Horiguchi G, Sugiyama M, Van Lijsebettens M, Lenhard M., J Plant Res 126(1), 2013
PMID: 22922868
Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO₂ levels.
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP., Plant J 73(5), 2013
PMID: 23181524
Functional Redundancy and Divergence within the Arabidopsis RETICULATA-RELATED Gene Family.
Pérez-Pérez JM, Esteve-Bruna D, González-Bayón R, Kangasjärvi S, Caldana C, Hannah MA, Willmitzer L, Ponce MR, Micol JL., Plant Physiol 162(2), 2013
PMID: 23596191
Mutation of an Arabidopsis NatB N-alpha-terminal acetylation complex component causes pleiotropic developmental defects.
Ferrández-Ayela A, Micol-Ponce R, Sánchez-García AB, Alonso-Peral MM, Micol JL, Ponce MR., PLoS One 8(11), 2013
PMID: 24244708

73 References

Daten bereitgestellt von Europe PubMed Central.

Root-synthesized cytokinin in Arabidopsis is distributed in the shoot by the transpiration stream.
Aloni R, Langhans M, Aloni E, Dreieicher E, Ullrich CI., J. Exp. Bot. 56(416), 2005
PMID: 15824073
Hormonal and cell division analyses in Watsonia lepida seedlings.
Ascough GD, Novak O, Pencik A, Rolcik J, Strnad M, Erwin JE, Van Staden J., J. Plant Physiol. 166(14), 2009
PMID: 19423185
The role of proteins in C(3) plants prior to their recruitment into the C(4) pathway.
Aubry S, Brown NJ, Hibberd JM., J. Exp. Bot. 62(9), 2011
PMID: 21321052
Conjugates of auxin and cytokinin.
Bajguz A, Piotrowska A., Phytochemistry 70(8), 2009
PMID: 19524990
A mutational analysis of leaf morphogenesis in Arabidopsis thaliana.
Berna G, Robles P, Micol JL., Genetics 152(2), 1999
PMID: 10353913
Venation pattern formation in Arabidopsis thaliana vegetative leaves.
Candela H, Martinez-Laborda A, Micol JL., Dev. Biol. 205(1), 1999
PMID: 9882508
Characterization of the response of the Arabidopsis response regulator gene family to cytokinin.
D'Agostino IB, Deruere J, Kieber JJ., Plant Physiol. 124(4), 2000
PMID: 11115887
Proline biosynthesis and osmoregulation in plants
Delauney, Plant J 4(), 1993
Morphogenesis of simple and compound leaves: a critical review.
Efroni I, Eshed Y, Lifschitz E., Plant Cell 22(4), 2010
PMID: 20435903
Predicting subcellular localization of proteins based on their N-terminal amino acid sequence.
Emanuelsson O, Nielsen H, Brunak S, von Heijne G., J. Mol. Biol. 300(4), 2000
PMID: 10891285
Metabolite profiling in Arabidopsis
Fiehn, 2006
A new class of plastidic phosphate translocators: a putative link between primary and secondary metabolism by the phosphoenolpyruvate/phosphate antiporter.
Fischer K, Kammerer B, Gutensohn M, Arbinger B, Weber A, Hausler RE, Flugge UI., Plant Cell 9(3), 1997
PMID: 9090886
Seed and vascular expression of a high-affinity transporter for cationic amino acids in Arabidopsis.
Frommer WB, Hummel S, Unseld M, Ninnemann O., Proc. Natl. Acad. Sci. U.S.A. 92(26), 1995
PMID: 8618839
Mutations in the RETICULATA gene dramatically alter internal architecture but have little effect on overall organ shape in Arabidopsis leaves.
Gonzalez-Bayon R, Kinsman EA, Quesada V, Vera A, Robles P, Ponce MR, Pyke KA, Micol JL., J. Exp. Bot. 57(12), 2006
PMID: 16873448
Evolution of C4 photosynthesis in the genus Flaveria: how many and which genes does it take to make C4?
Gowik U, Brautigam A, Weber KL, Weber AP, Westhoff P., Plant Cell 23(6), 2011
PMID: 21705644
Avoiding bias in calculations of relative growth rate.
Hoffmann WA, Poorter H., Ann. Bot. 90(1), 2002
PMID: 12125771
Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development.
Horiguchi G, Molla-Morales A, Perez-Perez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H., Plant J. 65(5), 2011
PMID: 21251100
Characterization of Arabidopsis glutamine phosphoribosyl pyrophosphate amidotransferase-deficient mutants.
Hung WF, Chen LJ, Boldt R, Sun CW, Li HM., Plant Physiol. 135(3), 2004
PMID: 15266056
Arabidopsis map-based cloning in the post-genome era.
Jander G, Norris SR, Rounsley SD, Bush DF, Levin IM, Last RL., Plant Physiol. 129(2), 2002
PMID: 12068090
Simultaneous phenotyping of leaf growth and chlorophyll fluorescence via GROWSCREEN FLUORO allows detection of stress tolerance in Arabidopsis thaliana and other rosette plants
Jansen Marcus, Gilmer Frank, Biskup Bernhard, Nagel KerstinA, Rascher Uwe, Fischbach Andreas, Briem Sabine, Dreissen Georg, Tittmann Susanne, Braun Silvia, De Jaeger Iris, Metzlaff Michael, Schurr Ulrich, Scharr Hanno, Walter Achim., Funct. Plant Biol. 36(10-1), 2009
PMID: IND44281535
Differential positioning of C(4) mesophyll and bundle sheath chloroplasts: recovery of chloroplast positioning requires the actomyosin system.
Kobayashi H, Yamada M, Taniguchi M, Kawasaki M, Sugiyama T, Miyake H., Plant Cell Physiol. 50(1), 2008
PMID: 19022806
Asparagine in plants
Lea PJ, Sodek L, Parry MAJ, Shewry PR, Halford NG., Ann. Appl. Biol. 150(1), 2007
PMID: IND43874542
Cytokinins: synthesis, mass spectra, and biological activity of compounds related to zeatin.
Leonard NJ, Hecht SM, Skoog F, Schmitz RY., Proc. Natl. Acad. Sci. U.S.A. 63(1), 1969
PMID: 16591745
Structure-activity-relationships of cytokinins
Matsubara, Phytochem 19(), 1980
Auxin signaling in Arabidopsis leaf vascular development.
Mattsson J, Ckurshumova W, Berleth T., Plant Physiol. 131(3), 2003
PMID: 12644682
Identification and expression analysis of twelve members of the nucleobase-ascorbate transporter (NAT) gene family in Arabidopsis thaliana.
Maurino VG, Grube E, Zielinski J, Schild A, Fischer K, Flugge UI., Plant Cell Physiol. 47(10), 2006
PMID: 16982705
Cytokinin metabolism and action
Mok, Ann. Rev. Plant Physiol. Plant Mol. Biol. 52(), 2001
Analysis of ven3 and ven6 reticulate mutants reveals the importance of arginine biosynthesis in Arabidopsis leaf development.
Molla-Morales A, Sarmiento-Manus R, Robles P, Quesada V, Perez-Perez JM, Gonzalez-Bayon R, Hannah MA, Willmitzer L, Ponce MR, Micol JL., Plant J. 65(3), 2010
PMID: 21265888
Cytokinin-auxin crosstalk.
Moubayidin L, Di Mambro R, Sabatini S., Trends Plant Sci. 14(10), 2009
PMID: 19734082
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Murashige, Physiol. Plant. 15(), 1962
The evolution and functional significance of leaf shape in the angiosperms
Nicotra, Func. Plant Biol. 38(), 2011
Identification of cytokinins associated with mitosis in synchronously cultured tobacco cells
Nishinari, Plant Cell Physiol 21(), 1980
Cytokinin profiling in plant tissues using ultra-performance liquid chromatography-electrospray tandem mass spectrometry.
Novak O, Hauserova E, Amakorova P, Dolezal K, Strnad M., Phytochemistry 69(11), 2008
PMID: 18561963
Quantitative analysis of cytokinins in plants by liquid chromatography-single-quadrupole mass spectrometry
Novák, Anal. Chim. Acta 480(), 2003
Prospects for crop production under drought: research priorities and future directions.
Parry MAJ, Flexas J, Medrano H., Ann. Appl. Biol. 147(3), 2005
PMID: IND43794202
Determination of accurate extinction coefficients and simultaneous-equations for assaying chlorophyll a and chlorophyll b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy
Porra, Biochim. Biophys. Acta 975(), 1989
Temporal and spatial development of the cells of the expanding first leaf of Arabidopsis thaliana (L.) heynh
Pyke, J. Exp. Bot 42(), 1991
Linkage studies
Rédei, Arabidopsis Information Service 1(), 1964
Levels of endogenous cytokinins, indole-3-acetic acid and abscisic acid during the cell cycle of synchronized tobacco BY-2 cells
Redig, FBS Lett. 391(), 1996
Urea, ureides, and guanidines in plants
Reinbothe, Annu. Rev. Plant Biol. 13(), 1962
The RON1/FRY1/SAL1 gene is required for leaf morphogenesis and venation patterning in Arabidopsis.
Robles P, Fleury D, Candela H, Cnops G, Alonso-Peral MM, Anami S, Falcone A, Caldana C, Willmitzer L, Ponce MR, Van Lijsebettens M, Micol JL., Plant Physiol. 152(3), 2009
PMID: 20044451
Vein patterning in growing leaves: axes and polarities.
Rolland-Lagan AG., Curr. Opin. Genet. Dev. 18(4), 2008
PMID: 18606536
Cytokinins: activity, biosynthesis, and translocation
Sakakibara, Ann. Rev. Plant Biol 57(), 2006
Molecular organization of the shikimate pathway in higher plants
Schmid, Phytochem 39(), 1995
Comparison of cytokinin activities of naturally occurring ribonuclease
Schmitz, Phytochem 11(), 1972
Cytokinin-Dependent Expression of the ARR5::GUS Construct during Transgenic Arabidopsis Growth
Shtratnikova, Russian Journal of Plant Physiology 55(), 2008
Purine biosynthesis. Big in cell division, even bigger in nitrogen assimilation.
Smith PM, Atkins CA., Plant Physiol. 128(3), 2002
PMID: 11891236
The phosphoenolpyruvate/phosphate translocator is required for phenolic metabolism, palisade cell development, and plastid-dependent nuclear gene expression.
Streatfield SJ, Weber A, Kinsman EA, Hausler RE, Li J, Post-Beittenmiller D, Kaiser WM, Pyke KA, Flugge UI, Chory J., Plant Cell 11(9), 1999
PMID: 10488230
The Arabidopsis Information Resource (TAIR): gene structure and function annotation
Swarbreck, Nucl. Acids Res. 36(), 2008
Molecular analysis of ‘de novo’ purine biosynthesis in solanaceous species and in Arabidopsis thaliana
van, Front. Biosci 9(), 2004
Transgenic Medicago truncatula plants that accumulate proline display nitrogen-fixing activity with enhanced tolerance to osmotic stress.
Verdoy D, Coba De La Pena T, Redondo FJ, Lucas MM, Pueyo JJ., Plant Cell Environ. 29(10), 2006
PMID: 16930317
The phenotype of the Arabidopsis cue1 mutant is not simply caused by a general restriction of the shikimate pathway.
Voll L, Hausler RE, Hecker R, Weber A, Weissenbock G, Fiene G, Waffenschmidt S, Flugge UI., Plant J. 36(3), 2003
PMID: 14617088
Chemical genetic identification of glutamine phosphoribosylpyrophosphate amidotransferase as the target for a novel bleaching herbicide in Arabidopsis.
Walsh TA, Bauer T, Neal R, Merlo AO, Schmitzer PR, Hicks GR, Honma M, Matsumura W, Wolff K, Davies JP., Plant Physiol. 144(3), 2007
PMID: 17616508
Dynamics of seedling growth acclimation towards altered light conditions can be quantified via GROWSCREEN: a setup and procedure designed for rapid optical phenotyping of different plant species.
Walter A, Scharr H, Gilmer F, Zierer R, Nagel KA, Ernst M, Wiese A, Virnich O, Christ MM, Uhlig B, Junger S, Schurr U., New Phytol. 174(2), 2007
PMID: 17388907
Using mutants to probe the in vivo function of plastid envelope membrane metabolite transporters.
Weber AP, Schneidereit J, Voll LM., J. Exp. Bot. 55(400), 2004
PMID: 15047758
An ‘Electronic Fluorescent Pictograph’ browser for exploring and analyzing large-scale biological data sets
Winter, PloS One 2(), 2007
Auxin biosynthesis and its role in plant development
Zhao, 2010
A functional analysis of the pyrimidine catabolic pathway in Arabidopsis.
Zrenner R, Riegler H, Marquard CR, Lange PR, Geserick C, Bartosz CE, Chen CT, Slocum RD., New Phytol. 183(1), 2009
PMID: 19413687
Pyrimidine and purine biosynthesis and degradation in plants.
Zrenner R, Stitt M, Sonnewald U, Boldt R., Annu Rev Plant Biol 57(), 2006
PMID: 16669783
Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis.
Mustroph A, Zanetti ME, Jang CJ, Holtan HE, Repetti PP, Galbraith DW, Girke T, Bailey-Serres J., Proc. Natl. Acad. Sci. U.S.A. 106(44), 2009
PMID: 19843695

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

PMID: 22532604
PubMed | Europe PMC

Suchen in

Google Scholar