Towards an integrative model of C-4 photosynthetic subtypes: insights from comparative transcriptome analysis of NAD-ME, NADP-ME, and PEP-CK C-4 species

Bräutigam A, Schliesky S, Külahoglu C, Osborne CP, Weber APM (2014)
Journal of Experimental Botany 65(13): 3579-3593.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
C-4 photosynthesis affords higher photosynthetic carbon conversion efficiency than C-3 photosynthesis and it therefore represents an attractive target for engineering efforts aiming to improve crop productivity. To this end, blueprints are required that reflect C-4 metabolism as closely as possible. Such blueprints have been derived from comparative transcriptome analyses of C-3 species with related C-4 species belonging to the NAD-malic enzyme (NAD-ME) and NADP-ME subgroups of C-4 photosynthesis. However, a comparison between C-3 and the phosphoenolpyruvate carboxykinase (PEP-CK) subtype of C-4 photosynthesis is still missing. An integrative analysis of all three C-4 subtypes has also not been possible to date, since no comparison has been available for closely related C-3 and PEP-CK C-4 species. To generate the data, the guinea grass Megathyrsus maximus, which represents a PEP-CK species, was analysed in comparison with a closely related C-3 sister species, Dichanthelium clandestinum, and with publicly available sets of RNA-Seq data from C-4 species belonging to the NAD-ME and NADP-ME subgroups. The data indicate that the core C-4 cycle of the PEP-CK grass M. maximus is quite similar to that of NAD-ME species with only a few exceptions, such as the subcellular location of transfer acid production and the degree and pattern of up-regulation of genes encoding C-4 enzymes. One additional mitochondrial transporter protein was associated with the core cycle. The broad comparison identified sucrose and starch synthesis, as well as the prevention of leakage of C-4 cycle intermediates to other metabolic pathways, as critical components of C-4 metabolism. Estimation of intercellular transport fluxes indicated that flux between cells is increased by at least two orders of magnitude in C-4 species compared with C-3 species. In contrast to NAD-ME and NADP-ME species, the transcription of photosynthetic electron transfer proteins was unchanged in PEP-CK. In summary, the PEP-CK blueprint of M. maximus appears to be simpler than those of NAD-ME and NADP-ME plants.
Erscheinungsjahr
Zeitschriftentitel
Journal of Experimental Botany
Band
65
Zeitschriftennummer
13
Seite
3579-3593
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Bräutigam A, Schliesky S, Külahoglu C, Osborne CP, Weber APM. Towards an integrative model of C-4 photosynthetic subtypes: insights from comparative transcriptome analysis of NAD-ME, NADP-ME, and PEP-CK C-4 species. Journal of Experimental Botany. 2014;65(13):3579-3593.
Bräutigam, A., Schliesky, S., Külahoglu, C., Osborne, C. P., & Weber, A. P. M. (2014). Towards an integrative model of C-4 photosynthetic subtypes: insights from comparative transcriptome analysis of NAD-ME, NADP-ME, and PEP-CK C-4 species. Journal of Experimental Botany, 65(13), 3579-3593. doi:10.1093/jxb/eru100
Bräutigam, A., Schliesky, S., Külahoglu, C., Osborne, C. P., and Weber, A. P. M. (2014). Towards an integrative model of C-4 photosynthetic subtypes: insights from comparative transcriptome analysis of NAD-ME, NADP-ME, and PEP-CK C-4 species. Journal of Experimental Botany 65, 3579-3593.
Bräutigam, A., et al., 2014. Towards an integrative model of C-4 photosynthetic subtypes: insights from comparative transcriptome analysis of NAD-ME, NADP-ME, and PEP-CK C-4 species. Journal of Experimental Botany, 65(13), p 3579-3593.
A. Bräutigam, et al., “Towards an integrative model of C-4 photosynthetic subtypes: insights from comparative transcriptome analysis of NAD-ME, NADP-ME, and PEP-CK C-4 species”, Journal of Experimental Botany, vol. 65, 2014, pp. 3579-3593.
Bräutigam, A., Schliesky, S., Külahoglu, C., Osborne, C.P., Weber, A.P.M.: Towards an integrative model of C-4 photosynthetic subtypes: insights from comparative transcriptome analysis of NAD-ME, NADP-ME, and PEP-CK C-4 species. Journal of Experimental Botany. 65, 3579-3593 (2014).
Bräutigam, Andrea, Schliesky, Simon, Külahoglu, Canan, Osborne, Colin P., and Weber, Andreas P. M. “Towards an integrative model of C-4 photosynthetic subtypes: insights from comparative transcriptome analysis of NAD-ME, NADP-ME, and PEP-CK C-4 species”. Journal of Experimental Botany 65.13 (2014): 3579-3593.
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31 Zitationen in Europe PMC

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Genetic enablers underlying the clustered evolutionary origins of C4 photosynthesis in angiosperms.
Christin PA, Arakaki M, Osborne CP, Edwards EJ., Mol Biol Evol 32(4), 2015
PMID: 25582594
An assessment of the capacity for phosphoenolpyruvate carboxykinase to contribute to C4 photosynthesis.
Koteyeva NK, Voznesenskaya EV, Edwards GE., Plant Sci 235(), 2015
PMID: 25900567
Insights into C4 metabolism from comparative deep sequencing.
Burgess SJ, Hibberd JM., Curr Opin Plant Biol 25(), 2015
PMID: 26051034
The evolutionary ecology of C4 plants.
Christin PA, Osborne CP., New Phytol 204(4), 2014
PMID: 25263843

81 References

Daten bereitgestellt von Europe PubMed Central.

Sampling the Arabidopsis transcriptome with massively parallel pyrosequencing.
Weber AP, Weber KL, Carr K, Wilkerson C, Ohlrogge JB., Plant Physiol. 144(1), 2007
PMID: 17351049
Complex RNA maturation in chloroplasts. The psbB operon from spinach.
Westhoff P, Herrmann RG., Eur. J. Biochem. 171(3), 1988
PMID: 2831053
Phosphoenolpyruvate carboxykinase is involved in the decarboxylation of aspartate in the bundle sheath of maize
Wingler A, Walker RP, Chen ZH, Leegood RC., Plant Physiol. 120(2), 1999
PMID: 10364405

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