Permeability modes in fluctuating lipid membranes with DNA-translocating pores

Moleiro LH, Mell M, Bocanegra R, Lopez-Montero I, Fouquet P, Hellweg T, Carrascosa JL, Monroy F (2017)
ADVANCES IN COLLOID AND INTERFACE SCIENCE 247: 543-554.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Moleiro, L. H.; Mell, M.; Bocanegra, R.; Lopez-Montero, I.; Fouquet, P.; Hellweg, ThomasUniBi ; Carrascosa, J. L.; Monroy, F.
Abstract / Bemerkung
Membrane pores can significantly alter not only the permeation dynamics of biological membranes but also their elasticity. Large membrane pores able to transport macromolecular contents represent an interesting model to test theoretical predictions that assign active-like (non-equilibrium) behavior to the permeability contributions to the enhanced membrane fluctuations existing in permeable membranes [Maneville et al. Phys. Rev. Lett. 82, 4356 (1999)]. Such high-amplitude active contributions arise from the forced transport of solvent and solutes through the open pores, which becomes even dominant at large permeability. In this paper, we present a detailed experimental analysis of the active shape fluctuations that appear in highly permeable lipid vesicles with large macromolecular pores inserted in the lipid membrane, which are a consequence of transport permeability events occurred in an osmotic gradient. The experimental results are found in quantitative agreement with theory, showing a remarkable dependence with the density of membrane pores and giving account of mechanical compliances and permeability rates that are compatible with the large size of the membrane pore considered. The presence of individual permeation events has been detected in the fluctuation time-series, from which a stochastic distribution of the permeation events compatible with a shot-noise has been deduced. The non equilibrium character of the membrane fluctuations in a permeation field, even if the membrane pores are mere passive transporters, is clearly demonstrated. Finally, a bio-nano-technology outlook of the proposed synthetic concept is given on the context of prospective uses as active membrane DNA-pores exploitable in gen-delivery applications based on lipid vesicles.
Stichworte
Neutron spin-echo; Lipid vesicles; Bending elasticity; Membrane tension; Permeability modes; Membrane permeation dynamics
Erscheinungsjahr
2017
Zeitschriftentitel
ADVANCES IN COLLOID AND INTERFACE SCIENCE
Band
247
Seite(n)
543-554
ISSN
0001-8686
eISSN
1873-3727
Page URI
https://pub.uni-bielefeld.de/record/2914678

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Moleiro LH, Mell M, Bocanegra R, et al. Permeability modes in fluctuating lipid membranes with DNA-translocating pores. ADVANCES IN COLLOID AND INTERFACE SCIENCE. 2017;247:543-554.
Moleiro, L. H., Mell, M., Bocanegra, R., Lopez-Montero, I., Fouquet, P., Hellweg, T., Carrascosa, J. L., et al. (2017). Permeability modes in fluctuating lipid membranes with DNA-translocating pores. ADVANCES IN COLLOID AND INTERFACE SCIENCE, 247, 543-554. doi:10.1016/j.cis.2017.07.009
Moleiro, L. H., Mell, M., Bocanegra, R., Lopez-Montero, I., Fouquet, P., Hellweg, Thomas, Carrascosa, J. L., and Monroy, F. 2017. “Permeability modes in fluctuating lipid membranes with DNA-translocating pores”. ADVANCES IN COLLOID AND INTERFACE SCIENCE 247: 543-554.
Moleiro, L. H., Mell, M., Bocanegra, R., Lopez-Montero, I., Fouquet, P., Hellweg, T., Carrascosa, J. L., and Monroy, F. (2017). Permeability modes in fluctuating lipid membranes with DNA-translocating pores. ADVANCES IN COLLOID AND INTERFACE SCIENCE 247, 543-554.
Moleiro, L.H., et al., 2017. Permeability modes in fluctuating lipid membranes with DNA-translocating pores. ADVANCES IN COLLOID AND INTERFACE SCIENCE, 247, p 543-554.
L.H. Moleiro, et al., “Permeability modes in fluctuating lipid membranes with DNA-translocating pores”, ADVANCES IN COLLOID AND INTERFACE SCIENCE, vol. 247, 2017, pp. 543-554.
Moleiro, L.H., Mell, M., Bocanegra, R., Lopez-Montero, I., Fouquet, P., Hellweg, T., Carrascosa, J.L., Monroy, F.: Permeability modes in fluctuating lipid membranes with DNA-translocating pores. ADVANCES IN COLLOID AND INTERFACE SCIENCE. 247, 543-554 (2017).
Moleiro, L. H., Mell, M., Bocanegra, R., Lopez-Montero, I., Fouquet, P., Hellweg, Thomas, Carrascosa, J. L., and Monroy, F. “Permeability modes in fluctuating lipid membranes with DNA-translocating pores”. ADVANCES IN COLLOID AND INTERFACE SCIENCE 247 (2017): 543-554.
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