Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids

Bhambhani S, Lakhwani D, Gupta P, Pandey A, Dhar YV, Bag SK, Asif MH, Trivedi PK (2017)
SCIENTIFIC REPORTS 7(1): 5043.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Bhambhani, Sweta; Lakhwani, Deepika; Gupta, Parul; Pandey, AshutoshUniBi; Dhar, Yogeshwar Vikram; Bag, Sumit Kumar; Asif, Mehar Hasan; Trivedi, Prabodh Kumar
Abstract / Bemerkung
Azadirachta indica A. Juss, commonly known as Neem, is the reservoir of triterpenoids of economic importance. Metabolite analysis of different developmental stages of leaf and fruit suggests tissue-specific accumulation of the major triterpenoids in this important tree. Though biosynthesis of these complex molecules requires substrate flux from the isoprenoid pathway, enzymes involved in late biosynthetic steps remain uncharacterized. We established and analyzed transcriptome datasets from leaf and fruit and identified members of gene families involved in intermediate steps of terpenoid backbone biosynthesis and those related to secondary transformation leading to the tissue-specific triterpenoid biosynthesis. Expression analysis suggests differential expression of number of genes between leaf and fruit and probable participation in the biosynthesis of fruit-specific triterpenoids. Genome-wide analysis also identified members of gene families putatively involved in secondary modifications in late biosynthetic steps leading to the synthesis of highly oxygenated triterpenoids. Expression and molecular docking analyses suggest involvement of specific members of CYP450 family in secondary modifications for the biosynthesis of bioactive triterpenoids. This study generated rich genomic resource and identified genes involved in biosynthesis of important molecules, which will aid in the advancement of tools for functional genomics and elucidation of the biosynthesis of triterpenoid from this important tree.
Erscheinungsjahr
2017
Zeitschriftentitel
SCIENTIFIC REPORTS
Band
7
Ausgabe
1
Art.-Nr.
5043
ISSN
2045-2322
Page URI
https://pub.uni-bielefeld.de/record/2913152

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Bhambhani S, Lakhwani D, Gupta P, et al. Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids. SCIENTIFIC REPORTS. 2017;7(1): 5043.
Bhambhani, S., Lakhwani, D., Gupta, P., Pandey, A., Dhar, Y. V., Bag, S. K., Asif, M. H., et al. (2017). Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids. SCIENTIFIC REPORTS, 7(1), 5043. doi:10.1038/s41598-017-05291-3
Bhambhani, Sweta, Lakhwani, Deepika, Gupta, Parul, Pandey, Ashutosh, Dhar, Yogeshwar Vikram, Bag, Sumit Kumar, Asif, Mehar Hasan, and Trivedi, Prabodh Kumar. 2017. “Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids”. SCIENTIFIC REPORTS 7 (1): 5043.
Bhambhani, S., Lakhwani, D., Gupta, P., Pandey, A., Dhar, Y. V., Bag, S. K., Asif, M. H., and Trivedi, P. K. (2017). Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids. SCIENTIFIC REPORTS 7:5043.
Bhambhani, S., et al., 2017. Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids. SCIENTIFIC REPORTS, 7(1): 5043.
S. Bhambhani, et al., “Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids”, SCIENTIFIC REPORTS, vol. 7, 2017, : 5043.
Bhambhani, S., Lakhwani, D., Gupta, P., Pandey, A., Dhar, Y.V., Bag, S.K., Asif, M.H., Trivedi, P.K.: Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids. SCIENTIFIC REPORTS. 7, : 5043 (2017).
Bhambhani, Sweta, Lakhwani, Deepika, Gupta, Parul, Pandey, Ashutosh, Dhar, Yogeshwar Vikram, Bag, Sumit Kumar, Asif, Mehar Hasan, and Trivedi, Prabodh Kumar. “Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids”. SCIENTIFIC REPORTS 7.1 (2017): 5043.

1 Zitation in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Agrobiotechnology Goes Wild: Ancient Local Varieties as Sources of Bioactives.
Berni R, Cantini C, Romi M, Hausman JF, Guerriero G, Cai G., Int J Mol Sci 19(8), 2018
PMID: 30071603

34 References

Daten bereitgestellt von Europe PubMed Central.

Meliaceous limonoids: chemistry and biological activities.
Tan QG, Luo XD., Chem. Rev. 111(11), 2011
PMID: 21894902
Expedient preparative isolation and tandem mass spectrometric characterization of C-seco triterpenoids from Neem oil.
Haldar S, Mulani FA, Aarthy T, Dandekar DS, Thulasiram HV., J Chromatogr A 1366(), 2014
PMID: 25267707
Triterpenoid profiling and functional characterization of the initial genes involved in isoprenoid biosynthesis in neem (Azadirachta indica).
Pandreka A, Dandekar DS, Haldar S, Uttara V, Vijayshree SG, Mulani FA, Aarthy T, Thulasiram HV., BMC Plant Biol. 15(), 2015
PMID: 26335498
Comprehensive analyses of genomes, transcriptomes and metabolites of neem tree.
Kuravadi NA, Yenagi V, Rangiah K, Mahesh HB, Rajamani A, Shirke MD, Russiachand H, Loganathan RM, Shankara Lingu C, Siddappa S, Ramamurthy A, Sathyanarayana BN, Gowda M., PeerJ 3(), 2015
PMID: 26290780
Clinical trial with praneem polyherbal cream in patients with abnormal vaginal discharge due to microbial infections.
Mittal A, Kapur S, Garg S, Upadhyay SN, Suri S, Das SK, Gupta S, Talwar GP., Aust N Z J Obstet Gynaecol 35(2), 1995
PMID: 7677686
Characterization and mosquitocidal potential of neem cake-synthesized silver nanoparticles: genotoxicity and impact on predation efficiency of mosquito natural enemies.
Chandramohan B, Murugan K, Panneerselvam C, Madhiyazhagan P, Chandirasekar R, Dinesh D, Kumar PM, Kovendan K, Suresh U, Subramaniam J, Rajaganesh R, Aziz AT, Syuhei B, Alsalhi MS, Devanesan S, Nicoletti M, Wei H, Benelli G., Parasitol. Res. 115(3), 2015
PMID: 26573518
Azadirachta indica triterpenoids promote osteoblast differentiation and mineralization in vitro and in vivo.
Kushwaha P, Khedgikar V, Haldar S, Gautam J, Mulani FA, Thulasiram HV, Trivedi R., Bioorg. Med. Chem. Lett. 26(15), 2016
PMID: 27317644

AUTHOR UNKNOWN, 0
Tetracyclic triterpenoids and their derivatives from Azadirachta indica
Siddiqui S, Siddiqui BS, Faizi S, Mahmood T., 1988
Comparative transcriptome analysis of different chemotypes elucidates withanolide biosynthesis pathway from medicinal plant Withania somnifera.
Gupta P, Goel R, Agarwal AV, Asif MH, Sangwan NS, Sangwan RS, Trivedi PK., Sci Rep 5(), 2015
PMID: 26688389
Development of the major triterpenoids and oil in the fruit and seeds of Neem (Azadirachta indica)
Johnson S, Morgan D, Peiris C., 1996
The chemistry of azadirachtin
Ley SV, Denhom AA, Wood A., 1993

AUTHOR UNKNOWN, 0
A draft of the genome and four transcriptomes of a medicinal and pesticidal angiosperm Azadirachta indica.
Krishnan NM, Pattnaik S, Jain P, Gaur P, Choudhary R, Vaidyanathan S, Deepak S, Hariharan AK, Krishna PB, Nair J, Varghese L, Valivarthi NK, Dhas K, Ramaswamy K, Panda B., BMC Genomics 13(), 2012
PMID: 22958331
Subtractive transcriptomes of fruit and leaf reveal differential representation of transcripts in Azadirachta indica
Rajakani R, Narnoliya L, Sangwan NS, Sangwan RS, Gupta V., 2014
An Improved Genome Assembly of Azadirachta indica A. Juss.
Krishnan NM, Jain P, Gupta S, Hariharan AK, Panda B., G3 (Bethesda) 6(7), 2016
PMID: 27172223

AUTHOR UNKNOWN, 0
CYP83b1 is the oxime-metabolizing enzyme in the glucosinolate pathway in Arabidopsis.
Hansen CH, Du L, Naur P, Olsen CE, Axelsen KB, Hick AJ, Pickett JA, Halkier BA., J. Biol. Chem. 276(27), 2001
PMID: 11333274
(+)-Abscisic acid 8'-hydroxylase is a cytochrome P450 monooxygenase
Krochko JE, Abrams GD, Loewen MK, Abrams SR, Cutler AJ., Plant Physiol. 118(3), 1998
PMID: 9808729
Comparative interactions of withanolides and sterols with two members of sterol glycosyltransferases from Withania somnifera.
Pandey V, Dhar YV, Gupta P, Bag SK, Atri N, Asif MH, Trivedi PK, Misra P., BMC Bioinformatics 16(), 2015
PMID: 25888493
Biochemical analysis of a multifunctional cytochrome P450 (CYP51) enzyme required for synthesis of antimicrobial triterpenes in plants.
Geisler K, Hughes RK, Sainsbury F, Lomonossoff GP, Rejzek M, Fairhurst S, Olsen CE, Motawia MS, Melton RE, Hemmings AM, Bak S, Osbourn A., Proc. Natl. Acad. Sci. U.S.A. 110(35), 2013
PMID: 23940321
The comparison of gene expression from multiple cDNA libraries.
Stekel DJ, Git Y, Falciani F., Genome Res. 10(12), 2000
PMID: 11116099
De novo sequencing and assembly of Azadirachta indica fruit transcriptome
Krishnan NM., 2011
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S., Mol. Biol. Evol. 28(10), 2011
PMID: 21546353
The Phyre2 web portal for protein modeling, prediction and analysis.
Kelley LA, Mezulis S, Yates CM, Wass MN, Sternberg MJ., Nat Protoc 10(6), 2015
PMID: 25950237
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