Isoprenoid pyrophosphate-dependent transcriptional regulation of carotenogenesis in Corynebacterium glutamicum

Henke NA, Heider S, Hannibal S, Wendisch VF, Peters-Wendisch P (2017)
Frontiers in Microbiology 8: 633.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 2.16 MB
Abstract / Bemerkung
Corynebacterium glutamicum is a natural producer of the C50 carotenoid decaprenoxanthin. The crtEcg0722crtBIYEb operon comprises most of its genes for terpenoid biosynthesis. The MarR-type regulator encoded upstream and in divergent orientation of the carotenoid biosynthesis operon has not yet been characterized. This regulator, named CrtR in this study, is encoded in many actinobacterial genomes co-occurring with terpenoid biosynthesis genes. CrtR was shown to repress the crt operon of C. glutamicum since DNA microarray experiments revealed that transcript levels of crt operon genes were increased 10 to 70-fold in its absence. Transcriptional fusions of a promoter-less gfp gene with the crt operon and crtR promoters confirmed that CrtR represses its own gene and the crt operon. Gel mobility shift assays with purified His-tagged CrtR showed that CrtR binds to a region overlapping with the −10 and −35 promoter sequences of the crt operon. Isoprenoid pyrophosphates interfered with binding of CrtR to its target DNA, a so far unknown mechanism for regulation of carotenogenesis. The molecular details of protein-ligand interactions remain to be studied. Decaprenoxanthin synthesis by C. glutamicum wild type was enhanced 10 to 30-fold upon deletion of crtR and was decreased 5 to 6-fold as result of crtR overexpression. Moreover, deletion of crtR was shown as metabolic engineering strategy to improve production of native and non-native carotenoids including lycopene, β-carotene, C.p. 450 and sarcinaxanthin.
Erscheinungsjahr
2017
Zeitschriftentitel
Frontiers in Microbiology
Band
8
Art.-Nr.
633
ISSN
1664-302X
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Deutsche Forschungsgemeinschaft und die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2909045

Zitieren

Henke NA, Heider S, Hannibal S, Wendisch VF, Peters-Wendisch P. Isoprenoid pyrophosphate-dependent transcriptional regulation of carotenogenesis in Corynebacterium glutamicum. Frontiers in Microbiology. 2017;8: 633.
Henke, N. A., Heider, S., Hannibal, S., Wendisch, V. F., & Peters-Wendisch, P. (2017). Isoprenoid pyrophosphate-dependent transcriptional regulation of carotenogenesis in Corynebacterium glutamicum. Frontiers in Microbiology, 8, 633. doi:10.3389/fmicb.2017.00633
Henke, Nadja Alina, Heider, Sabine, Hannibal, Silvin, Wendisch, Volker F., and Peters-Wendisch, Petra. 2017. “Isoprenoid pyrophosphate-dependent transcriptional regulation of carotenogenesis in Corynebacterium glutamicum”. Frontiers in Microbiology 8: 633.
Henke, N. A., Heider, S., Hannibal, S., Wendisch, V. F., and Peters-Wendisch, P. (2017). Isoprenoid pyrophosphate-dependent transcriptional regulation of carotenogenesis in Corynebacterium glutamicum. Frontiers in Microbiology 8:633.
Henke, N.A., et al., 2017. Isoprenoid pyrophosphate-dependent transcriptional regulation of carotenogenesis in Corynebacterium glutamicum. Frontiers in Microbiology, 8: 633.
N.A. Henke, et al., “Isoprenoid pyrophosphate-dependent transcriptional regulation of carotenogenesis in Corynebacterium glutamicum”, Frontiers in Microbiology, vol. 8, 2017, : 633.
Henke, N.A., Heider, S., Hannibal, S., Wendisch, V.F., Peters-Wendisch, P.: Isoprenoid pyrophosphate-dependent transcriptional regulation of carotenogenesis in Corynebacterium glutamicum. Frontiers in Microbiology. 8, : 633 (2017).
Henke, Nadja Alina, Heider, Sabine, Hannibal, Silvin, Wendisch, Volker F., and Peters-Wendisch, Petra. “Isoprenoid pyrophosphate-dependent transcriptional regulation of carotenogenesis in Corynebacterium glutamicum”. Frontiers in Microbiology 8 (2017): 633.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
Dieses Objekt ist durch das Urheberrecht und/oder verwandte Schutzrechte geschützt. [...]
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-25T06:49:02Z
MD5 Prüfsumme
a639a4caca7a6a65ff625152a902d667


7 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Challenges and tackles in metabolic engineering for microbial production of carotenoids.
Wang C, Zhao S, Shao X, Park JB, Jeong SH, Park HJ, Kwak WJ, Wei G, Kim SW., Microb Cell Fact 18(1), 2019
PMID: 30885243
The MarR-Type Regulator MalR Is Involved in Stress-Responsive Cell Envelope Remodeling in Corynebacterium glutamicum.
Hünnefeld M, Persicke M, Kalinowski J, Frunzke J., Front Microbiol 10(), 2019
PMID: 31164873
Genomewide characterisation of the genetic diversity of carotenogenesis in bacteria of the order Sphingomonadales.
Siddaramappa S, Viswanathan V, Thiyagarajan S, Narjala A., Microb Genom 4(4), 2018
PMID: 29620507
Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum.
Henke NA, Wichmann J, Baier T, Frohwitter J, Lauersen KJ, Risse JM, Peters-Wendisch P, Kruse O, Wendisch VF., Genes (Basel) 9(4), 2018
PMID: 29673223
Transport and metabolic engineering of the cell factory Corynebacterium glutamicum.
Pérez-García F, Wendisch VF., FEMS Microbiol Lett 365(16), 2018
PMID: 29982619
Production of amino acids - Genetic and metabolic engineering approaches.
Lee JH, Wendisch VF., Bioresour Technol 245(pt b), 2017
PMID: 28552565

65 References

Daten bereitgestellt von Europe PubMed Central.

Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum.
Peters-Wendisch PG, Schiel B, Wendisch VF, Katsoulidis E, Mockel B, Sahm H, Eikmanns BJ., J. Mol. Microbiol. Biotechnol. 3(2), 2001
PMID: 11321586
Production of the Marine Carotenoid Astaxanthin by Metabolically Engineered Corynebacterium glutamicum.
Henke NA, Heider SA, Peters-Wendisch P, Wendisch VF., Mar Drugs 14(7), 2016
PMID: 27376307
An update on microbial carotenoid production: application of recent metabolic engineering tools.
Das A, Yoon SH, Lee SH, Kim JY, Oh DK, Kim SW., Appl. Microbiol. Biotechnol. 77(3), 2007
PMID: 17912511
Plasmid vectors for testing in vivo promoter activities in Corynebacterium glutamicum and Rhodococcus erythropolis.
Knoppova M, Phensaijai M, Vesely M, Zemanova M, Nesvera J, Patek M., Curr. Microbiol. 55(3), 2007
PMID: 17657537
Studies on transformation of Escherichia coli with plasmids.
Hanahan D., J. Mol. Biol. 166(4), 1983
PMID: 6345791
Transposon mutagenesis of Mycobacterium marinum identifies a locus linking pigmentation and intracellular survival.
Gao LY, Groger R, Cox JS, Beverley SM, Lawson EH, Brown EJ., Infect. Immun. 71(2), 2003
PMID: 12540574
Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production.
Stansen C, Uy D, Delaunay S, Eggeling L, Goergen JL, Wendisch VF., Appl. Environ. Microbiol. 71(10), 2005
PMID: 16204505
Adaptive response of Haloferax mediterranei to low concentrations of NaCl (< 20%) in the growth medium.
D'Souza SE, Altekar W, D'Souza SF., Arch. Microbiol. 168(1), 1997
PMID: 9211716
Comprehensive analysis of the Corynebacterium glutamicum transcriptome using an improved RNAseq technique.
Pfeifer-Sancar K, Mentz A, Ruckert C, Kalinowski J., BMC Genomics 14(), 2013
PMID: 24341750
Twilight zone of protein sequence alignments.
Rost B., Protein Eng. 12(2), 1999
PMID: 10195279
Carotenoid biosynthesis and biotechnological application.
Sandmann G., Arch. Biochem. Biophys. 385(1), 2001
PMID: 11361023
Metabolic engineering towards biotechnological production of carotenoids in microorganisms.
Lee PC, Schmidt-Dannert C., Appl. Microbiol. Biotechnol. 60(1-2), 2002
PMID: 12382037
Genetic makeup of the Corynebacterium glutamicum LexA regulon deduced from comparative transcriptomics and in vitro DNA band shift assays.
Jochmann N, Kurze AK, Czaja LF, Brinkrolf K, Brune I, Huser AT, Hansmeier N, Puhler A, Borovok I, Tauch A., Microbiology (Reading, Engl.) 155(Pt 5), 2009
PMID: 19372162
Microbial carotenoids.
Johnson EA, Schroeder WA., Adv. Biochem. Eng. Biotechnol. 53(), 1996
PMID: 8578971
Bacterial carotenoids. XXVI. C50-carotenoids. 2. Bacterioruberin.
Kelly M, Jensen SL., Acta Chem Scand 21(9), 1967
PMID: 5585680
Structural basis for gene regulation by a B12-dependent photoreceptor.
Jost M, Fernandez-Zapata J, Polanco MC, Ortiz-Guerrero JM, Chen PY, Kang G, Padmanabhan S, Elias-Arnanz M, Drennan CL., Nature 526(7574), 2015
PMID: 26416754
Detailed biosynthetic pathway to decaprenoxanthin diglucoside in Corynebacterium glutamicum and identification of novel intermediates.
Krubasik P, Takaichi S, Maoka T, Kobayashi M, Masamoto K, Sandmann G., Arch. Microbiol. 176(3), 2001
PMID: 11511870
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-β-d-Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum.
Binder D, Frohwitter J, Mahr R, Bier C, Grunberger A, Loeschcke A, Peters-Wendisch P, Kohlheyer D, Pietruszka J, Frunzke J, Jaeger KE, Wendisch VF, Drepper T., Appl. Environ. Microbiol. 82(20), 2016
PMID: 27520809
MarR family transcription factors.
Grove A., Curr. Biol. 23(4), 2013
PMID: 23428319
EMMA 2--a MAGE-compliant system for the collaborative analysis and integration of microarray data.
Dondrup M, Albaum SP, Griebel T, Henckel K, Junemann S, Kahlke T, Kleindt CK, Kuster H, Linke B, Mertens D, Mittard-Runte V, Neuweger H, Runte KJ, Tauch A, Tille F, Puhler A, Goesmann A., BMC Bioinformatics 10(), 2009
PMID: 19200358
LdrP, a cAMP receptor protein/FNR family transcriptional regulator, serves as a positive regulator for the light-inducible gene cluster in the megaplasmid of Thermus thermophilus.
Takano H, Agari Y, Hagiwara K, Watanabe R, Yamazaki R, Beppu T, Shinkai A, Ueda K., Microbiology (Reading, Engl.) 160(Pt 12), 2014
PMID: 25294106
Development of a Corynebacterium glutamicum DNA microarray and validation by genome-wide expression profiling during growth with propionate as carbon source.
Huser AT, Becker A, Brune I, Dondrup M, Kalinowski J, Plassmeier J, Puhler A, Wiegrabe I, Tauch A., J. Biotechnol. 106(2-3), 2003
PMID: 14651867
Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 for use as a platform strain for basic research and industrial biotechnology.
Baumgart M, Unthan S, Ruckert C, Sivalingam J, Grunberger A, Kalinowski J, Bott M, Noack S, Frunzke J., Appl. Environ. Microbiol. 79(19), 2013
PMID: 23892752
Genetic control for light-induced carotenoid production in non-phototrophic bacteria.
Takano H, Asker D, Beppu T, Ueda K., J. Ind. Microbiol. Biotechnol. 33(2), 2005
PMID: 16091943
Regulation of the malic enzyme gene malE by the transcriptional regulator MalR in Corynebacterium glutamicum.
Krause JP, Polen T, Youn JW, Emer D, Eikmanns BJ, Wendisch VF., J. Biotechnol. 159(3), 2012
PMID: 22261175
Complete biosynthetic pathway of the C50 carotenoid bacterioruberin from lycopene in the extremely halophilic archaeon Haloarcula japonica.
Yang Y, Yatsunami R, Ando A, Miyoko N, Fukui T, Takaichi S, Nakamura S., J. Bacteriol. 197(9), 2015
PMID: 25712483
Light-dependent gene regulation by a coenzyme B12-based photoreceptor.
Ortiz-Guerrero JM, Polanco MC, Murillo FJ, Padmanabhan S, Elias-Arnanz M., Proc. Natl. Acad. Sci. U.S.A. 108(18), 2011
PMID: 21502508
IdsA is the major geranylgeranyl pyrophosphate synthase involved in carotenogenesis in Corynebacterium glutamicum.
Heider SA, Peters-Wendisch P, Beekwilder J, Wendisch VF., FEBS J. 281(21), 2014
PMID: 25181035
Production and glucosylation of C50 and C 40 carotenoids by metabolically engineered Corynebacterium glutamicum.
Heider SA, Peters-Wendisch P, Netzer R, Stafnes M, Brautaset T, Wendisch VF., Appl. Microbiol. Biotechnol. 98(3), 2013
PMID: 24270893
Translation initiation with 70S ribosomes: an alternative pathway for leaderless mRNAs.
Moll I, Hirokawa G, Kiel MC, Kaji A, Blasi U., Nucleic Acids Res. 32(11), 2004
PMID: 15215335
Carotenoid biosynthesis and overproduction in Corynebacterium glutamicum.
Heider SA, Peters-Wendisch P, Wendisch VF., BMC Microbiol. 12(), 2012
PMID: 22963379
Adaptation of Corynebacterium glutamicum to ammonium limitation: a global analysis using transcriptome and proteome techniques.
Silberbach M, Schafer M, Huser AT, Kalinowski J, Puhler A, Kramer R, Burkovski A., Appl. Environ. Microbiol. 71(5), 2005
PMID: 15870326
Regulation of carotenoid biosynthesis.
Bramley PM, Mackenzie A., Curr. Top. Cell. Regul. 29(), 1988
PMID: 3293927
A novel regulatory gene for light-induced carotenoid synthesis in the bacterium Myxococcus xanthus.
Fontes M, Galbis-Martinez L, Murillo FJ., Mol. Microbiol. 47(2), 2003
PMID: 12519205
A bacterial antirepressor with SH3 domain topology mimics operator DNA in sequestering the repressor DNA recognition helix.
Leon E, Navarro-Aviles G, Santiveri CM, Flores-Flores C, Rico M, Gonzalez C, Murillo FJ, Elias-Arnanz M, Jimenez MA, Padmanabhan S., Nucleic Acids Res. 38(15), 2010
PMID: 20410074
Enzymatic assembly of DNA molecules up to several hundred kilobases.
Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO., Nat. Methods 6(5), 2009
PMID: 19363495
Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis.
Follmann M, Ochrombel I, Kramer R, Trotschel C, Poetsch A, Ruckert C, Huser A, Persicke M, Seiferling D, Kalinowski J, Marin K., BMC Genomics 10(), 2009
PMID: 20025733
Role and Function of LitR, an Adenosyl B12-Bound Light-Sensitive Regulator of Bacillus megaterium QM B1551, in Regulation of Carotenoid Production.
Takano H, Mise K, Hagiwara K, Hirata N, Watanabe S, Toriyabe M, Shiratori-Takano H, Ueda K., J. Bacteriol. 197(14), 2015
PMID: 25917914
Genomic position analyses and the transcription machinery.
Perez-Rueda E, Gralla JD, Collado-Vides J., J. Mol. Biol. 275(2), 1998
PMID: 9466899
EMMA: a platform for consistent storage and efficient analysis of microarray data.
Dondrup M, Goesmann A, Bartels D, Kalinowski J, Krause L, Linke B, Rupp O, Sczyrba A, Puhler A, Meyer F., J. Biotechnol. 106(2-3), 2003
PMID: 14651856
SigF controls carotenoid pigment production and affects transformation efficiency and hydrogen peroxide sensitivity in Mycobacterium smegmatis.
Provvedi R, Kocincova D, Dona V, Euphrasie D, Daffe M, Etienne G, Manganelli R, Reyrat JM., J. Bacteriol. 190(23), 2008
PMID: 18805974
Effects of Kasugamycin on the Translatome of Escherichia coli.
Lange C, Lehr M, Zerulla K, Ludwig P, Schweitzer J, Polen T, Wendisch VF, Soppa J., PLoS ONE 12(1), 2017
PMID: 28081129
Diversifying carotenoid biosynthetic pathways by directed evolution.
Umeno D, Tobias AV, Arnold FH., Microbiol. Mol. Biol. Rev. 69(1), 2005
PMID: 15755953
The crystal structure of MarR, a regulator of multiple antibiotic resistance, at 2.3 A resolution.
Alekshun MN, Levy SB, Mealy TR, Seaton BA, Head JF., Nat. Struct. Biol. 8(8), 2001
PMID: 11473263
Material in PUB:
Teil dieser Dissertation
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 28484430
PubMed | Europe PMC

Suchen in

Google Scholar