Comparative analysis of different xanthan samples by atomic force microscopy

Teckentrup J, Al-Hamood O, Steffens T, Bednarz H, Walhorn V, Niehaus K, Anselmetti D (2017)
Journal of Biotechnology 257: 2-8.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The polysaccharide xanthan which is produced by the gamma-proteobacterium Xanthomonas campestris is used as a food thickening agent and rheologic modifier in numerous food, cosmetics and technical applications. Its great commercial importance stimulated biotechnological approaches to optimize the xanthan production. By targeted genetic modification the metabolism of Xanthomonas can be modified in such a way that the xanthan production efficiency and/or the shear-thickening potency is optimized. Using atomic force microscopy (AFM) the secondary structure of single xanthan polymers produced by the wild type Xanthomonas campestris B100 and several genetically modified variations were analyzed. We found a wide variation of characteristic differences between xanthan molecules produced by different strains. The structures ranged from single-stranded coiled polymers to branched xanthan double-strands. These results can help to get a better understanding of the polymerization- and secretion-machinery that are relevant for xanthan synthesis. Furthermore, we demonstrate that the xanthan secondary structure strongly correlates with its viscosifying properties. Copyright A 2016 Elsevier B.V. All rights reserved.
Erscheinungsjahr
Zeitschriftentitel
Journal of Biotechnology
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257
Seite(n)
2-8
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Teckentrup J, Al-Hamood O, Steffens T, et al. Comparative analysis of different xanthan samples by atomic force microscopy. Journal of Biotechnology. 2017;257:2-8.
Teckentrup, J., Al-Hamood, O., Steffens, T., Bednarz, H., Walhorn, V., Niehaus, K., & Anselmetti, D. (2017). Comparative analysis of different xanthan samples by atomic force microscopy. Journal of Biotechnology, 257, 2-8. doi:10.1016/j.jbiotec.2016.11.032
Teckentrup, J., Al-Hamood, O., Steffens, T., Bednarz, H., Walhorn, V., Niehaus, K., and Anselmetti, D. (2017). Comparative analysis of different xanthan samples by atomic force microscopy. Journal of Biotechnology 257, 2-8.
Teckentrup, J., et al., 2017. Comparative analysis of different xanthan samples by atomic force microscopy. Journal of Biotechnology, 257, p 2-8.
J. Teckentrup, et al., “Comparative analysis of different xanthan samples by atomic force microscopy”, Journal of Biotechnology, vol. 257, 2017, pp. 2-8.
Teckentrup, J., Al-Hamood, O., Steffens, T., Bednarz, H., Walhorn, V., Niehaus, K., Anselmetti, D.: Comparative analysis of different xanthan samples by atomic force microscopy. Journal of Biotechnology. 257, 2-8 (2017).
Teckentrup, Julia, Al-Hamood, Orooba, Steffens, Tim, Bednarz, Hanna, Walhorn, Volker, Niehaus, Karsten, and Anselmetti, Dario. “Comparative analysis of different xanthan samples by atomic force microscopy”. Journal of Biotechnology 257 (2017): 2-8.

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Refined annotation of the complete genome of the phytopathogenic and xanthan producing Xanthomonas campestris pv. campestris strain B100 based on RNA sequence data.
Alkhateeb RS, Rückert C, Rupp O, Pucker B, Hublik G, Wibberg D, Niehaus K, Pühler A, Vorhölter FJ., J Biotechnol 253(), 2017
PMID: 28506932

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