Survival rate of eukaryotic cells following electrophoretic nanoinjection

Simonis M, Hübner W, Wilking A, Huser T, Hennig S (2017)
Scientific Reports 7: 41277.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 1.15 MB
Abstract / Bemerkung
Insertion of foreign molecules such as functionalized fluorescent probes, antibodies, or plasmid DNA to living cells requires overcoming the plasma membrane barrier without harming the cell during the staining process. Many techniques such as electroporation, lipofection or microinjection have been developed to overcome the cellular plasma membrane, but they all result in reduced cell viability. A novel approach is the injection of cells with a nanopipette and using electrophoretic forces for the delivery of molecules. The tip size of these pipettes is approximately ten times smaller than typical microinjection pipettes and rather than pressure pulses as delivery method, moderate DC electric fields are used to drive charged molecules out of the tip. Here, we show that this approach leads to a significantly higher survival rate of nanoinjected cells and that injection with nanopipettes has a significantly lower impact on the proliferation behavior of injected cells. Thus, we propose that injection with nanopipettes using electrophoretic delivery is an excellent alternative when working with valuable and rare living cells, such as primary cells or stem cells.
Erscheinungsjahr
2017
Zeitschriftentitel
Scientific Reports
Band
7
Art.-Nr.
41277
ISSN
2045-2322
Finanzierungs-Informationen
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
Page URI
https://pub.uni-bielefeld.de/record/2908228

Zitieren

Simonis M, Hübner W, Wilking A, Huser T, Hennig S. Survival rate of eukaryotic cells following electrophoretic nanoinjection. Scientific Reports. 2017;7: 41277.
Simonis, M., Hübner, W., Wilking, A., Huser, T., & Hennig, S. (2017). Survival rate of eukaryotic cells following electrophoretic nanoinjection. Scientific Reports, 7, 41277. doi:10.1038/srep41277
Simonis, M., Hübner, W., Wilking, A., Huser, T., and Hennig, S. (2017). Survival rate of eukaryotic cells following electrophoretic nanoinjection. Scientific Reports 7:41277.
Simonis, M., et al., 2017. Survival rate of eukaryotic cells following electrophoretic nanoinjection. Scientific Reports, 7: 41277.
M. Simonis, et al., “Survival rate of eukaryotic cells following electrophoretic nanoinjection”, Scientific Reports, vol. 7, 2017, : 41277.
Simonis, M., Hübner, W., Wilking, A., Huser, T., Hennig, S.: Survival rate of eukaryotic cells following electrophoretic nanoinjection. Scientific Reports. 7, : 41277 (2017).
Simonis, Matthias, Hübner, Wolfgang, Wilking, Alice, Huser, Thomas, and Hennig, Simon. “Survival rate of eukaryotic cells following electrophoretic nanoinjection”. Scientific Reports 7 (2017): 41277.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0):
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-06T09:18:43Z
MD5 Prüfsumme
b719d7567932db959ca294d5db014a5f

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 28120926
PubMed | Europe PMC

Suchen in

Google Scholar