The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana

Wagner L, Schmal C, Staiger D, Danisman S (2017)
Plant Methods 13: 2.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 2.31 MB
Abstract / Bemerkung
Background The analysis of circadian leaf movement rhythms is a simple yet effective method to study effects of treatments or gene mutations on the circadian clock of plants. Currently, leaf movements are analysed using time lapse photography and subsequent bioinformatics analyses of leaf movements. Programs that are used for this purpose either are able to perform one function (i.e. leaf tip detection or rhythm analysis) or their function is limited to specific computational environments. We developed a leaf movement analysis tool—PALMA—that works in command line and combines image extraction with rhythm analysis using Fast Fourier transformation and non-linear least squares fitting. Results We validated PALMA in both simulated time series and in experiments using the known short period mutant sensitivity to red light reduced 1 (srr1-1). We compared PALMA with two established leaf movement analysis tools and found it to perform equally well. Finally, we tested the effect of reduced iron conditions on the leaf movement rhythms of wild type plants. Here, we found that PALMA successfully detected period lengthening under reduced iron conditions. Conclusions PALMA correctly estimated the period of both simulated and real-life leaf movement experiments. As a platform-independent console-program that unites both functions needed for the analysis of circadian leaf movements it is a valid alternative to existing leaf movement analysis tools.
Stichworte
Circadian clock Iron homeostasis Rhythmic leaf movement analysis Rhythmic cotyledon movement analysis PALMA
Erscheinungsjahr
2017
Zeitschriftentitel
Plant Methods
Band
13
Art.-Nr.
2
ISSN
1746-4811
Finanzierungs-Informationen
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
Page URI
https://pub.uni-bielefeld.de/record/2907785

Zitieren

Wagner L, Schmal C, Staiger D, Danisman S. The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana. Plant Methods. 2017;13: 2.
Wagner, L., Schmal, C., Staiger, D., & Danisman, S. (2017). The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana. Plant Methods, 13, 2. doi:10.1186/s13007-016-0153-3
Wagner, L., Schmal, C., Staiger, D., and Danisman, S. (2017). The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana. Plant Methods 13:2.
Wagner, L., et al., 2017. The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana. Plant Methods, 13: 2.
L. Wagner, et al., “The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana”, Plant Methods, vol. 13, 2017, : 2.
Wagner, L., Schmal, C., Staiger, D., Danisman, S.: The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana. Plant Methods. 13, : 2 (2017).
Wagner, Lucas, Schmal, Christoph, Staiger, Dorothee, and Danisman, Selahattin. “The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana”. Plant Methods 13 (2017): 2.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2019-09-06T09:18:42Z
MD5 Prüfsumme
c073c20c0f47f82044ab471a877a3838

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 28053647
PubMed | Europe PMC

Suchen in

Google Scholar