Functional architecture and global properties of the Corynebacterium glutamicum regulatory network: Novel insights from a dataset with a high genomic coverage

Freyre-González JA, Tauch A (2017)
Journal of Biotechnology 257: 199-210.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
 
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Autor/in
Freyre-González, Julio A.; Tauch, AndreasUniBi
Abstract / Bemerkung
Corynebacterium glutamicum is a Gram-positive, anaerobic, rod-shaped soil bacterium able to grow on a diversity of carbon sources like sugars and organic acids. It is a biotechnological relevant organism because of its highly efficient ability to biosynthesize amino acids, such as l-glutamic acid and l-lysine. Here, we reconstructed the most complete C. glutamicum regulatory network to date and comprehensively analyzed its global organizational properties, systems-level features and functional architecture. Our analyses show the tremendous power of Abasy Atlas to study the functional organization of regulatory networks. We created two models of the C. glutamicum regulatory network: all-evidences (containing both weak and strong supported interactions, genomic coverage = 73%) and strongly-supported (only accounting for strongly supported evidences, genomic coverage = 71%). Using state-of-the-art methodologies, we prove that power-law behaviors truly govern the connectivity and clustering coefficient distributions. We found a non-previously reported circuit motif that we named complex feed-forward motif. We highlighted the importance of feedback loops for the functional architecture, beyond whether they are statistically over-represented or not in the network. We show that the previously reported top-down approach is inadequate to infer the hierarchy governing a regulatory network because feedback bridges different hierarchical layers, and the top-down approach disregards the presence of intermodular genes shaping the integration layer. Our findings all together further support a diamond-shaped, three-layered hierarchy exhibiting some feedback between processing and coordination layers, which is shaped by four classes of systems-level elements: global regulators, locally autonomous modules, basal machinery and intermodular genes.
Stichworte
Corynebacterium glutamicum; Regulatory network; Global regulators; Modules; Intermodular genes; Functional architecture
Erscheinungsjahr
2017
Zeitschriftentitel
Journal of Biotechnology
Band
257
Seite(n)
199-210
ISSN
0168-1656
eISSN
1873-4863
Page URI
https://pub.uni-bielefeld.de/record/2907317

Zitieren

Freyre-González JA, Tauch A. Functional architecture and global properties of the Corynebacterium glutamicum regulatory network: Novel insights from a dataset with a high genomic coverage. Journal of Biotechnology. 2017;257:199-210.
Freyre-González, J. A., & Tauch, A. (2017). Functional architecture and global properties of the Corynebacterium glutamicum regulatory network: Novel insights from a dataset with a high genomic coverage. Journal of Biotechnology, 257, 199-210. doi:10.1016/j.jbiotec.2016.10.025
Freyre-González, J. A., and Tauch, A. (2017). Functional architecture and global properties of the Corynebacterium glutamicum regulatory network: Novel insights from a dataset with a high genomic coverage. Journal of Biotechnology 257, 199-210.
Freyre-González, J.A., & Tauch, A., 2017. Functional architecture and global properties of the Corynebacterium glutamicum regulatory network: Novel insights from a dataset with a high genomic coverage. Journal of Biotechnology, 257, p 199-210.
J.A. Freyre-González and A. Tauch, “Functional architecture and global properties of the Corynebacterium glutamicum regulatory network: Novel insights from a dataset with a high genomic coverage”, Journal of Biotechnology, vol. 257, 2017, pp. 199-210.
Freyre-González, J.A., Tauch, A.: Functional architecture and global properties of the Corynebacterium glutamicum regulatory network: Novel insights from a dataset with a high genomic coverage. Journal of Biotechnology. 257, 199-210 (2017).
Freyre-González, Julio A., and Tauch, Andreas. “Functional architecture and global properties of the Corynebacterium glutamicum regulatory network: Novel insights from a dataset with a high genomic coverage”. Journal of Biotechnology 257 (2017): 199-210.

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