Analyzing Synthetic Promoters Using Arabidopsis Protoplasts

Stracke, Ralf; Thiedig, Katharina; Kuhlmann, Melanie; Weisshaar, Bernd

Analyzing Synthetic Promoters Using Arabidopsis Protoplasts

Stracke R, Thiedig K, Kuhlmann M, Weisshaar B (2016)
In: Plant Synthetic Promoters. Hehl R (Ed); Methods in Molecular Biology, 1482. New York: Springer: 67-81.

Sammelwerksbeitrag | Veröffentlicht | Englisch

Download

Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!

URL

http://www.springer.com/de/book/9781493963942

Autor*in

Stracke, Ralf^UniBi ; Thiedig, Katharina^UniBi; Kuhlmann, Melanie^UniBi; Weisshaar, Bernd^UniBi

Herausgeber*in

Hehl, Reinhard

Einrichtung

Centrum für Biotechnologie > Arbeitsgruppe B. Weisshaar
Fakultät für Biologie > Genetik und Genomik der Pflanzen

Abstract / Bemerkung

This chapter describes a transient protoplast co-transfection method that can be used to quantitatively study in vivo the activity and function of promoters and promoter elements (reporters), and their induction or repression by transcription factors (effectors), stresses, hormones, or metabolites. A detailed protocol for carrying out transient co-transfection assays with Arabidopsis At7 protoplasts and calculating the promoter activity is provided.

Erscheinungsjahr

2016

Buchtitel

Plant Synthetic Promoters

Serientitel

Methods in Molecular Biology

Band

1482

Seite(n)

67-81

ISBN

978-1-4939-6394-2

Page URI

https://pub.uni-bielefeld.de/record/2904998

Zitieren

Stracke R, Thiedig K, Kuhlmann M, Weisshaar B. Analyzing Synthetic Promoters Using Arabidopsis Protoplasts. In: Hehl R, ed. Plant Synthetic Promoters. Methods in Molecular Biology. Vol 1482. New York: Springer; 2016: 67-81.

Stracke, R., Thiedig, K., Kuhlmann, M., & Weisshaar, B. (2016). Analyzing Synthetic Promoters Using Arabidopsis Protoplasts. In R. Hehl (Ed.), Methods in Molecular Biology: Vol. 1482. Plant Synthetic Promoters (pp. 67-81). New York: Springer.

Stracke, Ralf, Thiedig, Katharina, Kuhlmann, Melanie, and Weisshaar, Bernd. 2016. “Analyzing Synthetic Promoters Using Arabidopsis Protoplasts”. In Plant Synthetic Promoters, ed. Reinhard Hehl, 1482:67-81. Methods in Molecular Biology. New York: Springer.

Stracke, R., Thiedig, K., Kuhlmann, M., and Weisshaar, B. (2016). “Analyzing Synthetic Promoters Using Arabidopsis Protoplasts” in Plant Synthetic Promoters, Hehl, R. ed. Methods in Molecular Biology, vol. 1482, (New York: Springer), 67-81.

Stracke, R., et al., 2016. Analyzing Synthetic Promoters Using Arabidopsis Protoplasts. In R. Hehl, ed. Plant Synthetic Promoters. Methods in Molecular Biology. no.1482 New York: Springer, pp. 67-81.

R. Stracke, et al., “Analyzing Synthetic Promoters Using Arabidopsis Protoplasts”, Plant Synthetic Promoters, R. Hehl, ed., Methods in Molecular Biology, vol. 1482, New York: Springer, 2016, pp.67-81.

Stracke, R., Thiedig, K., Kuhlmann, M., Weisshaar, B.: Analyzing Synthetic Promoters Using Arabidopsis Protoplasts. In: Hehl, R. (ed.) Plant Synthetic Promoters. Methods in Molecular Biology. 1482, p. 67-81. Springer, New York (2016).

Stracke, Ralf, Thiedig, Katharina, Kuhlmann, Melanie, and Weisshaar, Bernd. “Analyzing Synthetic Promoters Using Arabidopsis Protoplasts”. Plant Synthetic Promoters. Ed. Reinhard Hehl. New York: Springer, 2016.Vol. 1482. Methods in Molecular Biology. 67-81.

Link(s) zu Volltext(en)

URL

http://www.springer.com/de/book/9781493963942

Access Level