Novel set-up for low-disturbance sampling of volatile and non-volatile compounds from plant roots

Eilers E, Pauls G, Rillig MC, Hansson BS, Hilker M, Reinecke A (2016)
Journal of Chemical Ecology 41(3): 253-266.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Eilers, ElisabethUniBi; Pauls, Gerhard; Rillig, Matthias C.; Hansson, Bill S.; Hilker, Monika; Reinecke, Andreas
Abstract / Bemerkung
Most studies on rhizosphere chemicals are carried out in substrate-free set-ups or in artificial substrates using sampling methods that require an air flow and may thus cause disturbance to the rhizosphere. Our study aimed to develop a simplified and inexpensive system that allows analysis of rhizosphere chemicals at experimentally less disturbed conditions. We designed a mesocosm in which volatile rhizosphere chemicals were sampled passively (by diffusion) without air- and water flow on polydimethylsiloxane-(PDMS) tubes. Dandelion (Taraxacum sect. ruderalia) was used as model plant; roots were left undamaged. Fifteen volatiles were retrieved from the sorptive material by thermal desorption for analysis by gas chromatography/mass spectrometry (GC/MS). Furthermore, three sugars were collected from the rhizosphere substrate by aqueous extraction and derivatized prior to GC/MS analysis. In order to study how the quantity of detected rhizosphere compounds depends on the type of soil or substrate, we determined the matrix-dependent recovery of synthetic rhizosphere chemicals. Furthermore, we compared sorption of volatiles on PDMS tubes with and without direct contact to the substrate. The results show that the newly designed mesocosm is suitable for low-invasive extraction of volatile and non-volatile compounds from rhizospheres. We further highlight how strongly the type of substrate and contact of PDMS tubes to the substrate affect the detectability of compounds from rhizospheres.
Stichworte
Root exudates; Gas chromatography/mass spectrometry; Root volatiles; Sugars; Soil substrates
Erscheinungsjahr
2016
Zeitschriftentitel
Journal of Chemical Ecology
Band
41
Ausgabe
3
Seite(n)
253-266
ISSN
0098-0331
eISSN
1573-1561
Page URI
https://pub.uni-bielefeld.de/record/2901393

Zitieren

Eilers E, Pauls G, Rillig MC, Hansson BS, Hilker M, Reinecke A. Novel set-up for low-disturbance sampling of volatile and non-volatile compounds from plant roots. Journal of Chemical Ecology. 2016;41(3):253-266.
Eilers, E., Pauls, G., Rillig, M. C., Hansson, B. S., Hilker, M., & Reinecke, A. (2016). Novel set-up for low-disturbance sampling of volatile and non-volatile compounds from plant roots. Journal of Chemical Ecology, 41(3), 253-266. doi:10.1007/s10886-015-0559-9
Eilers, E., Pauls, G., Rillig, M. C., Hansson, B. S., Hilker, M., and Reinecke, A. (2016). Novel set-up for low-disturbance sampling of volatile and non-volatile compounds from plant roots. Journal of Chemical Ecology 41, 253-266.
Eilers, E., et al., 2016. Novel set-up for low-disturbance sampling of volatile and non-volatile compounds from plant roots. Journal of Chemical Ecology, 41(3), p 253-266.
E. Eilers, et al., “Novel set-up for low-disturbance sampling of volatile and non-volatile compounds from plant roots”, Journal of Chemical Ecology, vol. 41, 2016, pp. 253-266.
Eilers, E., Pauls, G., Rillig, M.C., Hansson, B.S., Hilker, M., Reinecke, A.: Novel set-up for low-disturbance sampling of volatile and non-volatile compounds from plant roots. Journal of Chemical Ecology. 41, 253-266 (2016).
Eilers, Elisabeth, Pauls, Gerhard, Rillig, Matthias C., Hansson, Bill S., Hilker, Monika, and Reinecke, Andreas. “Novel set-up for low-disturbance sampling of volatile and non-volatile compounds from plant roots”. Journal of Chemical Ecology 41.3 (2016): 253-266.

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