Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*

Leßmeier L, Wendisch VF (2015)
BMC Microbiology 15: 216.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
Abstract / Bemerkung
Background Methanol is present in most ecosystems and may also occur in industrial applications, e.g. as an impurity of carbon sources such as technical glycerol. Methanol often inhibits growth of bacteria, thus, methanol tolerance may limit fermentative production processes. Results The methanol tolerance of the amino acid producing soil bacterium Corynebacterium glutamicum was improved by experimental evolution in the presence of methanol. The resulting strain Tol1 exhibited significantly increased growth rates in the presence of up to 1 M methanol. However, neither transcriptional changes nor increased enzyme activities of the linear methanol oxidation pathway were observed, which was in accordance with the finding that tolerance to the downstream metabolites formaldehyde and formate was not improved. Genome sequence analysis of strain Tol1 revealed two point mutations potentially relevant to enhanced methanol tolerance: one leading to the amino acid exchange A165T of O-acetylhomoserine sulfhydrolase MetY and the other leading to shortened CoA transferase Cat (Q342*). Introduction of either mutation into the genome of C. glutamicum wild type increased methanol tolerance and introduction of both mutations into C. glutamicum was sufficient to achieve methanol tolerance almost indistinguishable from that of strain Tol1. Conclusion The methanol tolerance of C. glutamicum can be increased by two point mutations leading to amino acid exchange of O-acetylhomoserine sulfhydrolase MetY and shortened CoA transferase Cat. Introduction of these mutations into producer strains may be helpful when using carbon sources containing methanol as component or impurity.
BMC Microbiology
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
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Leßmeier L, Wendisch VF. Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*. BMC Microbiology. 2015;15: 216.
Leßmeier, L., & Wendisch, V. F. (2015). Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*. BMC Microbiology, 15, 216. doi:10.1186/s12866-015-0558-6
Leßmeier, L., and Wendisch, V. F. (2015). Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*. BMC Microbiology 15:216.
Leßmeier, L., & Wendisch, V.F., 2015. Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*. BMC Microbiology, 15: 216.
L. Leßmeier and V.F. Wendisch, “Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*”, BMC Microbiology, vol. 15, 2015, : 216.
Leßmeier, L., Wendisch, V.F.: Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*. BMC Microbiology. 15, : 216 (2015).
Leßmeier, Lennart, and Wendisch, Volker F. “Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*”. BMC Microbiology 15 (2015): 216.
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Ester Formation by Alcohol Acetyltransferase from Brewers-Yeast
Yoshioka K, Hashimoto N., 1981
Regulation of acetate metabolism in Corynebacterium glutamicum: transcriptional control of the isocitrate lyase and malate synthase genes.
Wendisch VF, Spies M, Reinscheid DJ, Schnicke S, Sahm H, Eikmanns BJ., Arch. Microbiol. 168(4), 1997
PMID: 9297462
RamB, a novel transcriptional regulator of genes involved in acetate metabolism of Corynebacterium glutamicum.
Gerstmeir R, Cramer A, Dangel P, Schaffer S, Eikmanns BJ., J. Bacteriol. 186(9), 2004
PMID: 15090522
Studies on transformation of Escherichia coli with plasmids.
Hanahan D., J. Mol. Biol. 166(4), 1983
PMID: 6345791

Sambrook J, Russell D., 2001
Efficient electrotransformation of corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum plasmid pGA1.
Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J., Curr. Microbiol. 45(5), 2002
PMID: 12232668
Nucleotide sequence, expression and transcriptional analysis of the Corynebacterium glutamicum gltA gene encoding citrate synthase.
Eikmanns BJ, Thum-Schmitz N, Eggeling L, Ludtke KU, Sahm H., Microbiology (Reading, Engl.) 140 ( Pt 8)(), 1994
PMID: 7522844
C3-carboxylation as an anaplerotic reaction in phosphoenolpyruvate carboxylase-deficient Corynebacterium glutamicum.
Peters-Wendisch PG, Wendisch VF, de Graaf AA, Eikmanns BJ, Sahm H., Arch. Microbiol. 165(6), 1996
PMID: 8661932
Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis.
Polen T, Schluesener D, Poetsch A, Bott M, Wendisch VF., FEMS Microbiol. Lett. 273(1), 2007
PMID: 17559405
Exact and complete short-read alignment to microbial genomes using Graphics Processing Unit programming.
Blom J, Jakobi T, Doppmeier D, Jaenicke S, Kalinowski J, Stoye J, Goesmann A., Bioinformatics 27(10), 2011
PMID: 21450712
Gene Expression Omnibus: NCBI gene expression and hybridization array data repository.
Edgar R, Domrachev M, Lash AE., Nucleic Acids Res. 30(1), 2002
PMID: 11752295
Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum.
Peters-Wendisch PG, Schiel B, Wendisch VF, Katsoulidis E, Mockel B, Sahm H, Eikmanns BJ., J. Mol. Microbiol. Biotechnol. 3(2), 2001
PMID: 11321586


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