The Organic-Synthetic Potential of Recombinant Ene Reductases: Substrate-Scope Evaluation and Process Optimization

Reß T, Hummel W, Hanlon SP, Iding H, Gröger H (2015)
ChemCatChem 7(8): 1302-1311.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Reß, TinaUniBi; Hummel, WernerUniBi; Hanlon, Steven P.; Iding, Hans; Gröger, HaraldUniBi
Abstract / Bemerkung
In this study an evaluation of the synthetic potential of a broad range of recombinant ene reductases was performed. In detail, a library of 23 ene reductases was used to screen the C=C reduction of 21 activated alkenes from different compound classes as substrates. The chosen set of substrates comprises nitroalkenes with an aryl substituent at the beta-position and a methyl substituent at the alpha- or beta-position, alpha,beta-unsaturated carboxylic acids and their esters with and without substituents at the beta-position, a range of cyclic alpha,beta-unsaturated ketones with different ring sizes and substitution patterns and one alpha,beta-unsaturated boronic acid. After we obtained insight into the substrate scope, several biotransformations were prioritised and further investigated in a screening of 41 reaction parameters (which included chaotropic and kosmotropic salts, polyols, buffer solutions, amino acids and organic solvents) towards their impact on the activity and enantioselectivity of the applied ene reductases. Under the optimised conditions, selected reduction processes were performed on an increased lab scale (up to 30 mL) with up to 10% substrate concentration, which led in general to both high conversion and (if chiral products were formed) enantioselectivity.
Stichworte
reduction; enzyme catalysis; biotransformations; alkenes; asymmetric catalysis
Erscheinungsjahr
2015
Zeitschriftentitel
ChemCatChem
Band
7
Ausgabe
8
Seite(n)
1302-1311
ISSN
1867-3880
Page URI
https://pub.uni-bielefeld.de/record/2758474

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Reß T, Hummel W, Hanlon SP, Iding H, Gröger H. The Organic-Synthetic Potential of Recombinant Ene Reductases: Substrate-Scope Evaluation and Process Optimization. ChemCatChem. 2015;7(8):1302-1311.
Reß, T., Hummel, W., Hanlon, S. P., Iding, H., & Gröger, H. (2015). The Organic-Synthetic Potential of Recombinant Ene Reductases: Substrate-Scope Evaluation and Process Optimization. ChemCatChem, 7(8), 1302-1311. doi:10.1002/cctc.201402903
Reß, T., Hummel, W., Hanlon, S. P., Iding, H., and Gröger, H. (2015). The Organic-Synthetic Potential of Recombinant Ene Reductases: Substrate-Scope Evaluation and Process Optimization. ChemCatChem 7, 1302-1311.
Reß, T., et al., 2015. The Organic-Synthetic Potential of Recombinant Ene Reductases: Substrate-Scope Evaluation and Process Optimization. ChemCatChem, 7(8), p 1302-1311.
T. Reß, et al., “The Organic-Synthetic Potential of Recombinant Ene Reductases: Substrate-Scope Evaluation and Process Optimization”, ChemCatChem, vol. 7, 2015, pp. 1302-1311.
Reß, T., Hummel, W., Hanlon, S.P., Iding, H., Gröger, H.: The Organic-Synthetic Potential of Recombinant Ene Reductases: Substrate-Scope Evaluation and Process Optimization. ChemCatChem. 7, 1302-1311 (2015).
Reß, Tina, Hummel, Werner, Hanlon, Steven P., Iding, Hans, and Gröger, Harald. “The Organic-Synthetic Potential of Recombinant Ene Reductases: Substrate-Scope Evaluation and Process Optimization”. ChemCatChem 7.8 (2015): 1302-1311.

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