Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB
Sorger-Herrmann U, Taniguchi H, Wendisch VF (2015)
BMC Microbiology 15(1): 113.
Zeitschriftenaufsatz
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Einrichtung
Abstract / Bemerkung
Background
The pstSCAB operon of Corynebacterium glutamicum, which encodes an ABC transport system for uptake of phosphate (Pi), is induced during the Pi starvation response. The two-component regulatory system PhoRS is involved in this response, but partial Pi starvation induction of pstSCAB in a ΔphoRS mutant indicated the involvement of additional regulator(s). Regulation of pstSCAB also involves the global transcriptional regulator GlxR.
Results
DNA affinity chromatography identified the regulator of acetate metabolism RamB as a protein binding to pstS promoter DNA in vitro. Gel mobility shift assays and mutational analysis of the pstS promoter region revealed that RamB binds to two sites localized at positions −74 to −88 and −9 to +2 with respect to the transcriptional start site of pstSCAB. Reporter gene studies supported the in vivo relevance of both binding sites for activation of pstSCAB by RamB. DNA microarray analysis revealed that expression of many Pi starvation genes reached higher levels during the Pi starvation response on minimal medium with glucose as sole carbon source than in Pi starved acetate-grown C. glutamicum cells.
Conclusions
In C. glutamicum, RamB is involved in expression control of pstSCAB operon. Thus, transcriptional regulation of pstSCAB is complex involving activation by the phosphate-responsive two-component regulatory system PhoSR and the regulators of carbon metabolism GlxR and RamB.
Stichworte
Phosphate starvation;
Corynebacterium glutamicum;
pstS;
RamB;
Phosphorus metabolism;
Carbon metabolism;
Acetate metabolism;
PhoR;
GlxR
Erscheinungsjahr
2015
Zeitschriftentitel
BMC Microbiology
Band
15
Ausgabe
1
Art.-Nr.
113
ISSN
1471-2180
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Deutsche Forschungsgemeinschaft und die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2734072
Zitieren
Sorger-Herrmann U, Taniguchi H, Wendisch VF. Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB. BMC Microbiology. 2015;15(1): 113.
Sorger-Herrmann, U., Taniguchi, H., & Wendisch, V. F. (2015). Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB. BMC Microbiology, 15(1), 113. doi:10.1186/s12866-015-0437-1
Sorger-Herrmann, Ulrike, Taniguchi, Hironori, and Wendisch, Volker F. 2015. “Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB”. BMC Microbiology 15 (1): 113.
Sorger-Herrmann, U., Taniguchi, H., and Wendisch, V. F. (2015). Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB. BMC Microbiology 15:113.
Sorger-Herrmann, U., Taniguchi, H., & Wendisch, V.F., 2015. Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB. BMC Microbiology, 15(1): 113.
U. Sorger-Herrmann, H. Taniguchi, and V.F. Wendisch, “Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB”, BMC Microbiology, vol. 15, 2015, : 113.
Sorger-Herrmann, U., Taniguchi, H., Wendisch, V.F.: Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB. BMC Microbiology. 15, : 113 (2015).
Sorger-Herrmann, Ulrike, Taniguchi, Hironori, and Wendisch, Volker F. “Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB”. BMC Microbiology 15.1 (2015): 113.
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Daten bereitgestellt von European Bioinformatics Institute (EBI)
2 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
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