Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB

Sorger-Herrmann U, Taniguchi H, Wendisch VF (2015)
BMC Microbiology 15(1): 113.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Background The pstSCAB operon of Corynebacterium glutamicum, which encodes an ABC transport system for uptake of phosphate (Pi), is induced during the Pi starvation response. The two-component regulatory system PhoRS is involved in this response, but partial Pi starvation induction of pstSCAB in a ΔphoRS mutant indicated the involvement of additional regulator(s). Regulation of pstSCAB also involves the global transcriptional regulator GlxR. Results DNA affinity chromatography identified the regulator of acetate metabolism RamB as a protein binding to pstS promoter DNA in vitro. Gel mobility shift assays and mutational analysis of the pstS promoter region revealed that RamB binds to two sites localized at positions −74 to −88 and −9 to +2 with respect to the transcriptional start site of pstSCAB. Reporter gene studies supported the in vivo relevance of both binding sites for activation of pstSCAB by RamB. DNA microarray analysis revealed that expression of many Pi starvation genes reached higher levels during the Pi starvation response on minimal medium with glucose as sole carbon source than in Pi starved acetate-grown C. glutamicum cells. Conclusions In C. glutamicum, RamB is involved in expression control of pstSCAB operon. Thus, transcriptional regulation of pstSCAB is complex involving activation by the phosphate-responsive two-component regulatory system PhoSR and the regulators of carbon metabolism GlxR and RamB.
Stichworte
Phosphate starvation; Corynebacterium glutamicum; pstS; RamB; Phosphorus metabolism; Carbon metabolism; Acetate metabolism; PhoR; GlxR
Erscheinungsjahr
2015
Zeitschriftentitel
BMC Microbiology
Band
15
Ausgabe
1
Art.-Nr.
113
ISSN
1471-2180
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Deutsche Forschungsgemeinschaft und die Universität Bielefeld gefördert.
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https://pub.uni-bielefeld.de/record/2734072

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Sorger-Herrmann U, Taniguchi H, Wendisch VF. Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB. BMC Microbiology. 2015;15(1): 113.
Sorger-Herrmann, U., Taniguchi, H., & Wendisch, V. F. (2015). Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB. BMC Microbiology, 15(1), 113. doi:10.1186/s12866-015-0437-1
Sorger-Herrmann, Ulrike, Taniguchi, Hironori, and Wendisch, Volker F. 2015. “Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB”. BMC Microbiology 15 (1): 113.
Sorger-Herrmann, U., Taniguchi, H., and Wendisch, V. F. (2015). Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB. BMC Microbiology 15:113.
Sorger-Herrmann, U., Taniguchi, H., & Wendisch, V.F., 2015. Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB. BMC Microbiology, 15(1): 113.
U. Sorger-Herrmann, H. Taniguchi, and V.F. Wendisch, “Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB”, BMC Microbiology, vol. 15, 2015, : 113.
Sorger-Herrmann, U., Taniguchi, H., Wendisch, V.F.: Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB. BMC Microbiology. 15, : 113 (2015).
Sorger-Herrmann, Ulrike, Taniguchi, Hironori, and Wendisch, Volker F. “Regulation of the pstSCAB operon in Corynebacterium glutamicum by the regulator of acetate metabolism RamB”. BMC Microbiology 15.1 (2015): 113.
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2 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

GlnR positive transcriptional regulation of the phosphate-specific transport system pstSCAB in Amycolatopsis mediterranei U32.
Zhang Y, Zhang Y, Li P, Wang Y, Wang J, Shao Z, Zhao G., Acta Biochim Biophys Sin (Shanghai) 50(8), 2018
PMID: 30007316
Changes in protein abundance are observed in bacterial isolates from a natural host.
Rees MA, Stinear TP, Goode RJ, Coppel RL, Smith AI, Kleifeld O., Front Cell Infect Microbiol 5(), 2015
PMID: 26528441

65 References

Daten bereitgestellt von Europe PubMed Central.


Neidhardt FC., 1996

Sonenshein AL, Hoch JA, Losick R., 2002
Transcriptional regulation of the phoPR operon in Bacillus subtilis.
Pragai Z, Allenby NE, O'Connor N, Dubrac S, Rapoport G, Msadek T, Harwood CR., J. Bacteriol. 186(4), 2004
PMID: 14762014
Regulatory interactions between the Pho and sigma(B)-dependent general stress regulons of Bacillus subtilis.
Pragai Z, Harwood CR., Microbiology (Reading, Engl.) 148(Pt 5), 2002
PMID: 11988534
Genome-wide analysis of the general stress response in Bacillus subtilis.
Price CW, Fawcett P, Ceremonie H, Su N, Murphy CK, Youngman P., Mol. Microbiol. 41(4), 2001
PMID: 11532142
Taxonomical studies on glutamic acid-producing bacteria
Abe S, Takayama K-I, Kinoshita S., 1967
Industrial production of amino acids by coryneform bacteria.
Hermann T., J. Biotechnol. 104(1-3), 2003
PMID: 12948636
Biotechnological production of amino acids and derivatives: current status and prospects.
Leuchtenberger W, Huthmacher K, Drauz K., Appl. Microbiol. Biotechnol. 69(1), 2005
PMID: 16195792
Metabolic engineering of Corynebacterium glutamicum for L-serine production.
Peters-Wendisch P, Stolz M, Etterich H, Kennerknecht N, Sahm H, Eggeling L., Appl. Environ. Microbiol. 71(11), 2005
PMID: 16269752
l-Isoleucine Production with Corynebacterium glutamicum: Further Flux Increase and Limitation of Export.
Morbach S, Sahm H, Eggeling L., Appl. Environ. Microbiol. 62(12), 1996
PMID: 16535457
L-valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum.
Blombach B, Schreiner ME, Holatko J, Bartek T, Oldiges M, Eikmanns BJ., Appl. Environ. Microbiol. 73(7), 2007
PMID: 17293513
Linking central metabolism with increased pathway flux: L-valine accumulation by Corynebacterium glutamicum.
Radmacher E, Vaitsikova A, Burger U, Krumbach K, Sahm H, Eggeling L., Appl. Environ. Microbiol. 68(5), 2002
PMID: 11976094
Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum.
Jensen JV, Wendisch VF., Microb. Cell Fact. 12(), 2013
PMID: 23806148
Putrescine production by engineered Corynebacterium glutamicum.
Schneider J, Wendisch VF., Appl. Microbiol. Biotechnol. 88(4), 2010
PMID: 20661733
Metabolic engineering of Corynebacterium glutamicum for cadaverine fermentation.
Mimitsuka T, Sawai H, Hatsu M, Yamada K., Biosci. Biotechnol. Biochem. 71(9), 2007
PMID: 17895539
Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production.
Krause FS, Blombach B, Eikmanns BJ., Appl. Environ. Microbiol. 76(24), 2010
PMID: 20935122
Metabolic engineering of Corynebacterium glutamicum for 2-ketoisocaproate production.
Buckle-Vallant V, Krause FS, Messerschmidt S, Eikmanns BJ., Appl. Microbiol. Biotechnol. 98(1), 2013
PMID: 24169948

Vogt M, Haas S, Polen T, van J, Bott M., 2014
Phosphorus Metabolism
Wendisch VF, Bott M., 2005
Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum.
Klauth P, Pallerla SR, Vidaurre D, Ralfs C, Wendisch VF, Schoberth SM., Appl. Microbiol. Biotechnol. 72(5), 2006
PMID: 16977467
Monitoring of inorganic polyphosphate dynamics in Corynebacterium glutamicum using a novel oxygen sparger for real time 31P in vivo NMR
Lambert C, Weuster-Botz D, Weichenhain R, Kreutz EW, de AA, Schoberth SM., 2002
Formation of volutin granules in Corynebacterium glutamicum.
Pallerla SR, Knebel S, Polen T, Klauth P, Hollender J, Wendisch VF, Schoberth SM., FEMS Microbiol. Lett. 243(1), 2005
PMID: 15668011
NCgl2620 encodes a class II polyphosphate kinase in Corynebacterium glutamicum.
Lindner SN, Vidaurre D, Willbold S, Schoberth SM, Wendisch VF., Appl. Environ. Microbiol. 73(15), 2007
PMID: 17545325
Exopolyphosphatases PPX1 and PPX2 from Corynebacterium glutamicum.
Lindner SN, Knebel S, Wesseling H, Schoberth SM, Wendisch VF., Appl. Environ. Microbiol. 75(10), 2009
PMID: 19304823
Polyphosphate/ATP-dependent NAD kinase of Corynebacterium glutamicum: biochemical properties and impact of ppnK overexpression on lysine production.
Lindner SN, Niederholtmeyer H, Schmitz K, Schoberth SM, Wendisch VF., Appl. Microbiol. Biotechnol. 87(2), 2010
PMID: 20180116
Cg2091 encodes a polyphosphate/ATP-dependent glucokinase of Corynebacterium glutamicum.
Lindner SN, Knebel S, Pallerla SR, Schoberth SM, Wendisch VF., Appl. Microbiol. Biotechnol. 87(2), 2010
PMID: 20379711
The phosphate starvation stimulon of Corynebacterium glutamicum determined by DNA microarray analyses.
Ishige T, Krause M, Bott M, Wendisch VF, Sahm H., J. Bacteriol. 185(15), 2003
PMID: 12867461
Two-component systems of Corynebacterium glutamicum: deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response.
Kocan M, Schaffer S, Ishige T, Sorger-Herrmann U, Wendisch VF, Bott M., J. Bacteriol. 188(2), 2006
PMID: 16385062
High-resolution detection of DNA binding sites of the global transcriptional regulator GlxR in Corynebacterium glutamicum.
Jungwirth B, Sala C, Kohl TA, Uplekar S, Baumbach J, Cole ST, Puhler A, Tauch A., Microbiology (Reading, Engl.) 159(Pt 1), 2012
PMID: 23103979
The crystal structures of apo and cAMP-bound GlxR from Corynebacterium glutamicum reveal structural and dynamic changes upon cAMP binding in CRP/FNR family transcription factors.
Townsend PD, Jungwirth B, Pojer F, Bußmann M, Money VA, Cole ST, Puhler A, Tauch A, Bott M, Cann MJ, Pohl E., PLoS ONE 9(12), 2014
PMID: 25469635
The pstSCAB operon for phosphate uptake is regulated by the global regulator GlxR in Corynebacterium glutamicum.
Panhorst M, Sorger-Herrmann U, Wendisch VF., J. Biotechnol. 154(2-3), 2010
PMID: 20638427
Link between phosphate starvation and glycogen metabolism in Corynebacterium glutamicum, revealed by metabolomics.
Woo HM, Noack S, Seibold GM, Willbold S, Eikmanns BJ, Bott M., Appl. Environ. Microbiol. 76(20), 2010
PMID: 20802079
RamB, a novel transcriptional regulator of genes involved in acetate metabolism of Corynebacterium glutamicum.
Gerstmeir R, Cramer A, Dangel P, Schaffer S, Eikmanns BJ., J. Bacteriol. 186(9), 2004
PMID: 15090522
RamB is an activator of the pyruvate dehydrogenase complex subunit E1p gene in Corynebacterium glutamicum.
Blombach B, Cramer A, Eikmanns BJ, Schreiner M., J. Mol. Microbiol. Biotechnol. 16(3-4), 2007
PMID: 17890844
Citrate synthase in Corynebacterium glutamicum is encoded by two gltA transcripts which are controlled by RamA, RamB, and GlxR.
van Ooyen J, Emer D, Bussmann M, Bott M, Eikmanns BJ, Eggeling L., J. Biotechnol. 154(2-3), 2010
PMID: 20630483
Genome-wide requirements for Mycobacterium tuberculosis adaptation and survival in macrophages.
Rengarajan J, Bloom BR, Rubin EJ., Proc. Natl. Acad. Sci. U.S.A. 102(23), 2005
PMID: 15928073
Regulation of the phosphate regulon of Escherichia coli: characterization of the promoter of the pstS gene.
Kimura S, Makino K, Shinagawa H, Amemura M, Nakata A., Mol. Gen. Genet. 215(3), 1989
PMID: 2651888

Sambrook J., 2001
Integrative and autonomously replicating vectors for analysis of promoters in Corynebacterium glutamicum
Vasicová P, Abrhámová Z, Nesvera J, Pátek M, Sahm H, Eikmanns B., 1998
A high-resolution reference map for cytoplasmic and membrane-associated proteins of Corynebacterium glutamicum.
Schaffer S, Weil B, Nguyen VD, Dongmann G, Gunther K, Nickolaus M, Hermann T, Bott M., Electrophoresis 22(20), 2001
PMID: 11824608
Roles of pyruvate kinase and malic enzyme in Corynebacterium glutamicum for growth on carbon sources requiring gluconeogenesis.
Netzer R, Krause M, Rittmann D, Peters-Wendisch PG, Eggeling L, Wendisch VF, Sahm H., Arch. Microbiol. 182(5), 2004
PMID: 15375646
Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis.
Polen T, Schluesener D, Poetsch A, Bott M, Wendisch VF., FEMS Microbiol. Lett. 273(1), 2007
PMID: 17559405
Use of T7 RNA polymerase to direct expression of cloned genes.
Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW., Meth. Enzymol. 185(), 1990
PMID: 2199796
Studies on transformation of Escherichia coli with plasmids.
Hanahan D., J. Mol. Biol. 166(4), 1983
PMID: 6345791
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