Mutational definition of binding requirements of an hnRNP-like protein in Arabidopsis using fluorescence correlation spectroscopy

Leder V, Lummer M, Tegeler K, Humpert F, Lewinski M, Schüttpelz M, Staiger D (2014)
Biochemical and Biophysical Research Communications 453(1): 69-74.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Leder, VerenaUniBi; Lummer, MartinaUniBi; Tegeler, Kathrin; Humpert, FabianUniBi; Lewinski, MartinUniBi; Schüttpelz, MarkUniBi ; Staiger, DorotheeUniBi
Einrichtung
Fakultät für Biologie > RNA Biologie und Molekulare Physiologie
Fakultät für Physik
Abstract / Bemerkung
Arabidopsis thaliana glycine-rich RNA binding protein 7 (AtGRP7) is part of a negative feedback loop through which it regulates alternative splicing and steady-state abundance of its pre-mRNA. Here we use fluorescence correlation spectroscopy to investigate the requirements for AtGRP7 binding to its intron using fluorescently-labelled synthetic oligonucleotides. By systematically introducing point mutations we identify three nucleotides that lead to an increased K-d value when mutated and thus are critical for AtGRP7 binding. Simultaneous mutation of all three residues abrogates binding. The paralogue AtGRP8 binds to an overlapping motif but with a different sequence preference, in line with overlapping but not identical functions of this protein pair. Truncation of the glycine-rich domain reduces the binding affinity of AtGRP7, showing for the first time that the glycine-rich stretch of a plant hnRNP-like protein contributes to binding. Mutation of the conserved R-49 that is crucial for AtGRP7 function in pathogen defence and splicing abolishes binding. (C) 2014 Elsevier Inc. All rights reserved.
Stichworte
RNA-binding protein; RNA-protein-interaction; Fluorescence correlation spectroscopy
Erscheinungsjahr
2014
Zeitschriftentitel
Biochemical and Biophysical Research Communications
Band
453
Ausgabe
1
Seite(n)
69-74
ISSN
0006-291X
Page URI
https://pub.uni-bielefeld.de/record/2705568

Zitieren

Leder V, Lummer M, Tegeler K, et al. Mutational definition of binding requirements of an hnRNP-like protein in Arabidopsis using fluorescence correlation spectroscopy. Biochemical and Biophysical Research Communications. 2014;453(1):69-74.
Leder, V., Lummer, M., Tegeler, K., Humpert, F., Lewinski, M., Schüttpelz, M., & Staiger, D. (2014). Mutational definition of binding requirements of an hnRNP-like protein in Arabidopsis using fluorescence correlation spectroscopy. Biochemical and Biophysical Research Communications, 453(1), 69-74. doi:10.1016/j.bbrc.2014.09.056
Leder, Verena, Lummer, Martina, Tegeler, Kathrin, Humpert, Fabian, Lewinski, Martin, Schüttpelz, Mark, and Staiger, Dorothee. 2014. “Mutational definition of binding requirements of an hnRNP-like protein in Arabidopsis using fluorescence correlation spectroscopy”. Biochemical and Biophysical Research Communications 453 (1): 69-74.
Leder, V., Lummer, M., Tegeler, K., Humpert, F., Lewinski, M., Schüttpelz, M., and Staiger, D. (2014). Mutational definition of binding requirements of an hnRNP-like protein in Arabidopsis using fluorescence correlation spectroscopy. Biochemical and Biophysical Research Communications 453, 69-74.
Leder, V., et al., 2014. Mutational definition of binding requirements of an hnRNP-like protein in Arabidopsis using fluorescence correlation spectroscopy. Biochemical and Biophysical Research Communications, 453(1), p 69-74.
V. Leder, et al., “Mutational definition of binding requirements of an hnRNP-like protein in Arabidopsis using fluorescence correlation spectroscopy”, Biochemical and Biophysical Research Communications, vol. 453, 2014, pp. 69-74.
Leder, V., Lummer, M., Tegeler, K., Humpert, F., Lewinski, M., Schüttpelz, M., Staiger, D.: Mutational definition of binding requirements of an hnRNP-like protein in Arabidopsis using fluorescence correlation spectroscopy. Biochemical and Biophysical Research Communications. 453, 69-74 (2014).
Leder, Verena, Lummer, Martina, Tegeler, Kathrin, Humpert, Fabian, Lewinski, Martin, Schüttpelz, Mark, and Staiger, Dorothee. “Mutational definition of binding requirements of an hnRNP-like protein in Arabidopsis using fluorescence correlation spectroscopy”. Biochemical and Biophysical Research Communications 453.1 (2014): 69-74.

7 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Gene Mining for Proline Based Signaling Proteins in Cell Wall of Arabidopsis thaliana.
Ihsan MZ, Ahmad SJ, Shah ZH, Rehman HM, Aslam Z, Ahuja I, Bones AM, Ahmad JN., Front Plant Sci 8(), 2017
PMID: 28289422
RNA-Binding Proteins Revisited - The Emerging Arabidopsis mRNA Interactome.
Köster T, Marondedze C, Meyer K, Staiger D., Trends Plant Sci 22(6), 2017
PMID: 28412036
Adaptation of iCLIP to plants determines the binding landscape of the clock-regulated RNA-binding protein AtGRP7.
Meyer K, Köster T, Nolte C, Weinholdt C, Lewinski M, Grosse I, Staiger D., Genome Biol 18(1), 2017
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Lewinski M, Hallmann A, Staiger D., Mol Genet Genomics 291(2), 2016
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Li X, Xing J, Qiu Z, He Q, Lin J., Mol Plant 9(9), 2016
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In Planta Determination of the mRNA-Binding Proteome of Arabidopsis Etiolated Seedlings.
Reichel M, Liao Y, Rettel M, Ragan C, Evers M, Alleaume AM, Horos R, Hentze MW, Preiss T, Millar AA., Plant Cell 28(10), 2016
PMID: 27729395
Identification of the VERNALIZATION 4 gene reveals the origin of spring growth habit in ancient wheats from South Asia.
Kippes N, Debernardi JM, Vasquez-Gross HA, Akpinar BA, Budak H, Kato K, Chao S, Akhunov E, Dubcovsky J., Proc Natl Acad Sci U S A 112(39), 2015
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