Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato
Savidor A, Chalupowicz L, Teper D, Gartemann K-H, Eichenlaub R, Manulis-Sasson S, Barash I, Sessa G (2014)
Molecular Plant-Microbe Interactions 27(10): 1035-1047.
Zeitschriftenaufsatz
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Autor*in
Savidor, Alon;
Chalupowicz, Laura;
Teper, Doron;
Gartemann, Karl-HeinzUniBi
;
Eichenlaub, RudolfUniBi;
Manulis-Sasson, Shulamit;
Barash, Isaac;
Sessa, Guido
![](https://pub.uni-bielefeld.de/images/icon_orcid.png)
Einrichtung
Abstract / Bemerkung
The plant pathogen Clavibacter michiganensis subsp. michiganensis is a gram-positive bacterium responsible for wilt and canker disease of tomato. Although disease development is well characterized and diagnosed, molecular mechanisms of C. michiganensis subsp. michiganensis virulence are poorly understood. Here, we identified and characterized two C. michiganensis subsp. michiganensis transcriptional regulators, Vatr1 and Vatr2, that are involved in pathogenicity of C. michiganensis subsp. michiganensis. Vatr1 and Vatr2 belong to TetR and MocR families of transcriptional regulators, respectively. Mutations in their corresponding genes caused attenuated virulence, with the Delta vatr2 mutant showing a more dramatic effect than Delta vatr1. Although both mutants grew well in vitro and reached a high titer in planta, they caused reduced wilting and canker development in infected plants compared with the wild-type bacterium. They also led to a reduced expression of the ethylene-synthesizing tomato enzyme ACC-oxidase compared with wild-type C. michiganensis subsp. michiganensis and to reduced ethylene production in the plant. Transcriptomic analysis of wild-type C. michiganensis subsp. michiganensis and the two mutants under infectionmimicking conditions revealed that Vatr1 and Vatr2 regulate expression of virulence factors, membrane and secreted proteins, and signal-transducing proteins. A 70% overlap between the sets of genes positively regulated by Vatr1 and Vatr2 suggests that these transcriptional regulators are on the same molecular pathway responsible for C. michiganensis subsp. michiganensis virulence.
Erscheinungsjahr
2014
Zeitschriftentitel
Molecular Plant-Microbe Interactions
Band
27
Ausgabe
10
Seite(n)
1035-1047
ISSN
0894-0282
Page URI
https://pub.uni-bielefeld.de/record/2699455
Zitieren
Savidor A, Chalupowicz L, Teper D, et al. Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato. Molecular Plant-Microbe Interactions. 2014;27(10):1035-1047.
Savidor, A., Chalupowicz, L., Teper, D., Gartemann, K. - H., Eichenlaub, R., Manulis-Sasson, S., Barash, I., et al. (2014). Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato. Molecular Plant-Microbe Interactions, 27(10), 1035-1047. doi:10.1094/MPMI-02-14-0061-R
Savidor, Alon, Chalupowicz, Laura, Teper, Doron, Gartemann, Karl-Heinz, Eichenlaub, Rudolf, Manulis-Sasson, Shulamit, Barash, Isaac, and Sessa, Guido. 2014. “Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato”. Molecular Plant-Microbe Interactions 27 (10): 1035-1047.
Savidor, A., Chalupowicz, L., Teper, D., Gartemann, K. - H., Eichenlaub, R., Manulis-Sasson, S., Barash, I., and Sessa, G. (2014). Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato. Molecular Plant-Microbe Interactions 27, 1035-1047.
Savidor, A., et al., 2014. Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato. Molecular Plant-Microbe Interactions, 27(10), p 1035-1047.
A. Savidor, et al., “Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato”, Molecular Plant-Microbe Interactions, vol. 27, 2014, pp. 1035-1047.
Savidor, A., Chalupowicz, L., Teper, D., Gartemann, K.-H., Eichenlaub, R., Manulis-Sasson, S., Barash, I., Sessa, G.: Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato. Molecular Plant-Microbe Interactions. 27, 1035-1047 (2014).
Savidor, Alon, Chalupowicz, Laura, Teper, Doron, Gartemann, Karl-Heinz, Eichenlaub, Rudolf, Manulis-Sasson, Shulamit, Barash, Isaac, and Sessa, Guido. “Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato”. Molecular Plant-Microbe Interactions 27.10 (2014): 1035-1047.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
6 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Comparative Genomic Analyses of Clavibacter michiganensis subsp. insidiosus and Pathogenicity on Medicago truncatula.
Lu Y, Ishimaru CA, Glazebrook J, Samac DA., Phytopathology 108(2), 2018
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Lu Y, Ishimaru CA, Glazebrook J, Samac DA., Phytopathology 108(2), 2018
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Clavibacter michiganensis ssp. michiganensis: bacterial canker of tomato, molecular interactions and disease management.
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Chalupowicz L, Barash I, Reuven M, Dror O, Sharabani G, Gartemann KH, Eichenlaub R, Sessa G, Manulis-Sasson S., Mol Plant Pathol 18(3), 2017
PMID: 26992141
Chalupowicz L, Barash I, Reuven M, Dror O, Sharabani G, Gartemann KH, Eichenlaub R, Sessa G, Manulis-Sasson S., Mol Plant Pathol 18(3), 2017
PMID: 26992141
Genomic Analysis of Clavibacter michiganensis Reveals Insight Into Virulence Strategies and Genetic Diversity of a Gram-Positive Bacterial Pathogen.
Thapa SP, Pattathil S, Hahn MG, Jacques MA, Gilbertson RL, Coaker G., Mol Plant Microbe Interact 30(10), 2017
PMID: 28677494
Thapa SP, Pattathil S, Hahn MG, Jacques MA, Gilbertson RL, Coaker G., Mol Plant Microbe Interact 30(10), 2017
PMID: 28677494
A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants.
Hiery E, Poetsch A, Moosbauer T, Amin B, Hofmann J, Burkovski A., Proteomes 3(4), 2015
PMID: 28248277
Hiery E, Poetsch A, Moosbauer T, Amin B, Hofmann J, Burkovski A., Proteomes 3(4), 2015
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References
Daten bereitgestellt von Europe PubMed Central.
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