The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis
Lohr V, Haedicke O, Genzel Y, Jordan I, Büntemeyer H, Klamt S, Reichl U (2014)
BMC Biotechnology 14(1): 72.
Zeitschriftenaufsatz
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Autor*in
Lohr, Verena;
Haedicke, Oliver;
Genzel, Yvonne;
Jordan, Ingo;
Büntemeyer, HeinoUniBi;
Klamt, Steffen;
Reichl, Udo
Einrichtung
Abstract / Bemerkung
Background: In human vaccine manufacturing some pathogens such as Modified Vaccinia Virus Ankara, measles, mumps virus as well as influenza viruses are still produced on primary material derived from embryonated chicken eggs. Processes depending on primary cell culture, however, are difficult to adapt to modern vaccine production. Therefore, we derived previously a continuous suspension cell line, AGE1.CR.pIX, from muscovy duck and established chemically-defined media for virus propagation. Results: To better understand vaccine production processes, we developed a stoichiometric model of the central metabolism of AGE1.CR.pIX cells and applied flux variability and metabolic flux analysis. Results were compared to literature dealing with mammalian and insect cell culture metabolism focusing on the question whether cultured avian cells differ in metabolism. Qualitatively, the observed flux distribution of this avian cell line was similar to distributions found for mammalian cell lines (e.g. CHO, MDCK cells). In particular, glucose was catabolized inefficiently and glycolysis and TCA cycle seem to be only weakly connected. Conclusions: A distinguishing feature of the avian cell line is that glutaminolysis plays only a minor role in energy generation and production of precursors, resulting in low extracellular ammonia concentrations. This metabolic flux study is the first for a continuous avian cell line. It provides a basis for further metabolic analyses to exploit the biotechnological potential of avian and vertebrate cell lines and to develop specific optimized cell culture processes, e.g. vaccine production processes.
Stichworte
Glutaminolysis;
Metabolic network modeling;
analysis;
Flux variability;
Avian cell line AGE1.CR.pIX;
Biomass composition
Erscheinungsjahr
2014
Zeitschriftentitel
BMC Biotechnology
Band
14
Ausgabe
1
Art.-Nr.
72
ISSN
1472-6750
Page URI
https://pub.uni-bielefeld.de/record/2695008
Zitieren
Lohr V, Haedicke O, Genzel Y, et al. The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis. BMC Biotechnology. 2014;14(1): 72.
Lohr, V., Haedicke, O., Genzel, Y., Jordan, I., Büntemeyer, H., Klamt, S., & Reichl, U. (2014). The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis. BMC Biotechnology, 14(1), 72. doi:10.1186/1472-6750-14-72
Lohr, Verena, Haedicke, Oliver, Genzel, Yvonne, Jordan, Ingo, Büntemeyer, Heino, Klamt, Steffen, and Reichl, Udo. 2014. “The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis”. BMC Biotechnology 14 (1): 72.
Lohr, V., Haedicke, O., Genzel, Y., Jordan, I., Büntemeyer, H., Klamt, S., and Reichl, U. (2014). The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis. BMC Biotechnology 14:72.
Lohr, V., et al., 2014. The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis. BMC Biotechnology, 14(1): 72.
V. Lohr, et al., “The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis”, BMC Biotechnology, vol. 14, 2014, : 72.
Lohr, V., Haedicke, O., Genzel, Y., Jordan, I., Büntemeyer, H., Klamt, S., Reichl, U.: The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis. BMC Biotechnology. 14, : 72 (2014).
Lohr, Verena, Haedicke, Oliver, Genzel, Yvonne, Jordan, Ingo, Büntemeyer, Heino, Klamt, Steffen, and Reichl, Udo. “The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis”. BMC Biotechnology 14.1 (2014): 72.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
4 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Influenza viruses production: Evaluation of a novel avian cell line DuckCelt®-T17.
Petiot E, Proust A, Traversier A, Durous L, Dappozze F, Gras M, Guillard C, Balloul JM, Rosa-Calatrava M., Vaccine 36(22), 2018
PMID: 28571695
Petiot E, Proust A, Traversier A, Durous L, Dappozze F, Gras M, Guillard C, Balloul JM, Rosa-Calatrava M., Vaccine 36(22), 2018
PMID: 28571695
Bioreactors for high cell density and continuous multi-stage cultivations: options for process intensification in cell culture-based viral vaccine production.
Tapia F, Vázquez-Ramírez D, Genzel Y, Reichl U., Appl Microbiol Biotechnol 100(5), 2016
PMID: 26758296
Tapia F, Vázquez-Ramírez D, Genzel Y, Reichl U., Appl Microbiol Biotechnol 100(5), 2016
PMID: 26758296
Cell culture-based influenza vaccines: A necessary and indispensable investment for the future.
Hegde NR., Hum Vaccin Immunother 11(5), 2015
PMID: 25875691
Hegde NR., Hum Vaccin Immunother 11(5), 2015
PMID: 25875691
Designing cell lines for viral vaccine production: Where do we stand?
Genzel Y., Biotechnol J 10(5), 2015
PMID: 25903999
Genzel Y., Biotechnol J 10(5), 2015
PMID: 25903999
61 References
Daten bereitgestellt von Europe PubMed Central.
Optimisation of the cellular metabolism of glycosylation for recombinant proteins produced by Mammalian cell systems.
Butler M., Cytotechnology 50(1-3), 2006
PMID: 19003071
Butler M., Cytotechnology 50(1-3), 2006
PMID: 19003071
Engineering mammalian cells in bioprocessing - current achievements and future perspectives.
Lim Y, Wong NS, Lee YY, Ku SC, Wong DC, Yap MG., Biotechnol. Appl. Biochem. 55(4), 2010
PMID: 20392202
Lim Y, Wong NS, Lee YY, Ku SC, Wong DC, Yap MG., Biotechnol. Appl. Biochem. 55(4), 2010
PMID: 20392202
Modified vaccinia virus Ankara undergoes limited replication in human cells and lacks several immunomodulatory proteins: implications for use as a human vaccine.
Blanchard TJ, Alcami A, Andrea P, Smith GL., J. Gen. Virol. 79 ( Pt 5)(), 1998
PMID: 9603331
Blanchard TJ, Alcami A, Andrea P, Smith GL., J. Gen. Virol. 79 ( Pt 5)(), 1998
PMID: 9603331
Viruses as vaccine vectors for infectious diseases and cancer.
Draper SJ, Heeney JL., Nat. Rev. Microbiol. 8(1), 2010
PMID: 19966816
Draper SJ, Heeney JL., Nat. Rev. Microbiol. 8(1), 2010
PMID: 19966816
An avian cell line designed for production of highly attenuated viruses.
Jordan I, Vos A, Beilfuss S, Neubert A, Breul S, Sandig V., Vaccine 27(5), 2008
PMID: 19071186
Jordan I, Vos A, Beilfuss S, Neubert A, Breul S, Sandig V., Vaccine 27(5), 2008
PMID: 19071186
New avian suspension cell lines provide production of influenza virus and MVA in serum-free media: studies on growth, metabolism and virus propagation.
Lohr V, Rath A, Genzel Y, Jordan I, Sandig V, Reichl U., Vaccine 27(36), 2009
PMID: 19531390
Lohr V, Rath A, Genzel Y, Jordan I, Sandig V, Reichl U., Vaccine 27(36), 2009
PMID: 19531390
Human 293 cell metabolism in low glutamine-supplied culture: interpretation of metabolic changes through metabolic flux analysis.
Nadeau I, Sabatie J, Koehl M, Perrier M, Kamen A., Metab. Eng. 2(4), 2000
PMID: 11120640
Nadeau I, Sabatie J, Koehl M, Perrier M, Kamen A., Metab. Eng. 2(4), 2000
PMID: 11120640
293SF metabolic flux analysis during cell growth and infection with an adenoviral vector.
Nadeau I, Jacob D, Perrier M, Kamen A., Biotechnol. Prog. 16(5), 2000
PMID: 11027184
Nadeau I, Jacob D, Perrier M, Kamen A., Biotechnol. Prog. 16(5), 2000
PMID: 11027184
Quantitative characterization of metabolism and metabolic shifts during growth of the new human cell line AGE1.HN using time resolved metabolic flux analysis.
Niklas J, Schrader E, Sandig V, Noll T, Heinzle E., Bioprocess Biosyst Eng 34(5), 2010
PMID: 21188421
Niklas J, Schrader E, Sandig V, Noll T, Heinzle E., Bioprocess Biosyst Eng 34(5), 2010
PMID: 21188421
Metabolic flux rearrangement in the amino acid metabolism reduces ammonia stress in the α1-antitrypsin producing human AGE1.HN cell line.
Priesnitz C, Niklas J, Rose T, Sandig V, Heinzle E., Metab. Eng. 14(2), 2012
PMID: 22289170
Priesnitz C, Niklas J, Rose T, Sandig V, Heinzle E., Metab. Eng. 14(2), 2012
PMID: 22289170
Analysis of CHO cells metabolic redistribution in a glutamate-based defined medium in continuous culture.
Altamirano C, Illanes A, Casablancas A, Gamez X, Cairo JJ, Godia C., Biotechnol. Prog. 17(6), 2001
PMID: 11735437
Altamirano C, Illanes A, Casablancas A, Gamez X, Cairo JJ, Godia C., Biotechnol. Prog. 17(6), 2001
PMID: 11735437
Modelling and optimization of a recombinant BHK-21 cultivation process using hybrid grey-box systems.
Teixeira A, Cunha AE, Clemente JJ, Moreira JL, Cruz HJ, Alves PM, Carrondo MJ, Oliveira R., J. Biotechnol. 118(3), 2005
PMID: 16009442
Teixeira A, Cunha AE, Clemente JJ, Moreira JL, Cruz HJ, Alves PM, Carrondo MJ, Oliveira R., J. Biotechnol. 118(3), 2005
PMID: 16009442
Comparison of metabolic flux distributions for MDCK cell growth in glutamine- and pyruvate-containing media.
Sidorenko Y, Wahl A, Dauner M, Genzel Y, Reichl U., Biotechnol. Prog. 24(2), 2008
PMID: 18215054
Sidorenko Y, Wahl A, Dauner M, Genzel Y, Reichl U., Biotechnol. Prog. 24(2), 2008
PMID: 18215054
Metabolic flux model for an anchorage-dependent MDCK cell line: characteristic growth phases and minimum substrate consumption flux distribution.
Wahl A, Sidorenko Y, Dauner M, Genzel Y, Reichl U., Biotechnol. Bioeng. 101(1), 2008
PMID: 18646224
Wahl A, Sidorenko Y, Dauner M, Genzel Y, Reichl U., Biotechnol. Bioeng. 101(1), 2008
PMID: 18646224
Metabolic-flux analysis of hybridoma cells under oxidative and reductive stress using mass balances.
Bonarius HP, Houtman JH, Schmid G, de Gooijer CD, Tramper J., Cytotechnology 32(2), 2000
PMID: 19002972
Bonarius HP, Houtman JH, Schmid G, de Gooijer CD, Tramper J., Cytotechnology 32(2), 2000
PMID: 19002972
Metabolic flux analysis of hybridoma cells in different culture media using mass balances.
Bonarius HP, Hatzimanikatis V, Meesters KP, de Gooijer CD, Schmid G, Tramper J., Biotechnol. Bioeng. 50(3), 1996
PMID: 18626958
Bonarius HP, Hatzimanikatis V, Meesters KP, de Gooijer CD, Schmid G, Tramper J., Biotechnol. Bioeng. 50(3), 1996
PMID: 18626958
Cell density effect in the baculovirus-insect cells system: a quantitative analysis of energetic metabolism.
Bernal V, Carinhas N, Yokomizo AY, Carrondo MJ, Alves PM., Biotechnol. Bioeng. 104(1), 2009
PMID: 19459142
Bernal V, Carinhas N, Yokomizo AY, Carrondo MJ, Alves PM., Biotechnol. Bioeng. 104(1), 2009
PMID: 19459142
Baculovirus production for gene therapy: the role of cell density, multiplicity of infection and medium exchange.
Carinhas N, Bernal V, Yokomizo AY, Carrondo MJ, Oliveira R, Alves PM., Appl. Microbiol. Biotechnol. 81(6), 2008
PMID: 18923829
Carinhas N, Bernal V, Yokomizo AY, Carrondo MJ, Oliveira R, Alves PM., Appl. Microbiol. Biotechnol. 81(6), 2008
PMID: 18923829
Control and function of the transamination pathways of glutamine oxidation in tumour cells.
Kovacevic Z, Brkljac O, Bajin K., Biochem. J. 273(Pt 2)(), 1991
PMID: 1991025
Kovacevic Z, Brkljac O, Bajin K., Biochem. J. 273(Pt 2)(), 1991
PMID: 1991025
The pathways of glutamate and glutamine oxidation by tumor cell mitochondria. Role of mitochondrial NAD(P)+-dependent malic enzyme.
Moreadith RW, Lehninger AL., J. Biol. Chem. 259(10), 1984
PMID: 6144677
Moreadith RW, Lehninger AL., J. Biol. Chem. 259(10), 1984
PMID: 6144677
Evidence that glutamine, not sugar, is the major energy source for cultured HeLa cells.
Reitzer LJ, Wice BM, Kennell D., J. Biol. Chem. 254(8), 1979
PMID: 429309
Reitzer LJ, Wice BM, Kennell D., J. Biol. Chem. 254(8), 1979
PMID: 429309
Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells.
Glacken MW, Fleischaker RJ, Sinskey AJ., Biotechnol. Bioeng. 28(9), 1986
PMID: 18561227
Glacken MW, Fleischaker RJ, Sinskey AJ., Biotechnol. Bioeng. 28(9), 1986
PMID: 18561227
A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: effect of nutrient concentration, dilution rate, and pH.
Miller WM, Blanch HW, Wilke CR., Biotechnol. Bioeng. 32(8), 1988
PMID: 18587813
Miller WM, Blanch HW, Wilke CR., Biotechnol. Bioeng. 32(8), 1988
PMID: 18587813
Metabolic flux analysis and pharmaceutical production.
Boghigian BA, Seth G, Kiss R, Pfeifer BA., Metab. Eng. 12(2), 2009
PMID: 19861167
Boghigian BA, Seth G, Kiss R, Pfeifer BA., Metab. Eng. 12(2), 2009
PMID: 19861167
Metabolic flux analysis in mammalian cell culture.
Quek LE, Dietmair S, Kromer JO, Nielsen LK., Metab. Eng. 12(2), 2009
PMID: 19833223
Quek LE, Dietmair S, Kromer JO, Nielsen LK., Metab. Eng. 12(2), 2009
PMID: 19833223
Metabolic flux analysis of CHO cells at growth and non-growth phases using isotopic tracers and mass spectrometry.
Ahn WS, Antoniewicz MR., Metab. Eng. 13(5), 2011
PMID: 21821143
Ahn WS, Antoniewicz MR., Metab. Eng. 13(5), 2011
PMID: 21821143
The effects of alternate optimal solutions in constraint-based genome-scale metabolic models.
Mahadevan R, Schilling CH., Metab. Eng. 5(4), 2003
PMID: 14642354
Mahadevan R, Schilling CH., Metab. Eng. 5(4), 2003
PMID: 14642354
Structural and functional analysis of cellular networks with CellNetAnalyzer.
Klamt S, Saez-Rodriguez J, Gilles ED., BMC Syst Biol 1(), 2007
PMID: 17408509
Klamt S, Saez-Rodriguez J, Gilles ED., BMC Syst Biol 1(), 2007
PMID: 17408509
Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal cells in bioreactor.
Xie L, Wang DI., Cytotechnology 15(1-3), 1994
PMID: 7765929
Xie L, Wang DI., Cytotechnology 15(1-3), 1994
PMID: 7765929
Intracellular flux analysis in hybridomas using mass balances and in vitro (13)C nmr.
Zupke C, Stephanopoulos G., Biotechnol. Bioeng. 45(4), 1995
PMID: 18623182
Zupke C, Stephanopoulos G., Biotechnol. Bioeng. 45(4), 1995
PMID: 18623182
Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels.
Carnicer M, Baumann K, Toplitz I, Sanchez-Ferrando F, Mattanovich D, Ferrer P, Albiol J., Microb. Cell Fact. 8(), 2009
PMID: 20003217
Carnicer M, Baumann K, Toplitz I, Sanchez-Ferrando F, Mattanovich D, Ferrer P, Albiol J., Microb. Cell Fact. 8(), 2009
PMID: 20003217
Combined in silico modeling and metabolomics analysis to characterize fed-batch CHO cell culture.
Selvarasu S, Ho YS, Chong WP, Wong NS, Yusufi FN, Lee YY, Yap MG, Lee DY., Biotechnol. Bioeng. 109(6), 2012
PMID: 22252269
Selvarasu S, Ho YS, Chong WP, Wong NS, Yusufi FN, Lee YY, Yap MG, Lee DY., Biotechnol. Bioeng. 109(6), 2012
PMID: 22252269
Utilization of glucose and amino acids in insect cell cultures: Quantifying the metabolic flows within the primary pathways and medium development.
Ferrance JP, Goel A, Ataai MM., Biotechnol. Bioeng. 42(6), 1993
PMID: 18613102
Ferrance JP, Goel A, Ataai MM., Biotechnol. Bioeng. 42(6), 1993
PMID: 18613102
Fed-batch cultivation of animal cells using different medium design concepts and feeding strategies.
Xie L, Wang DI., Biotechnol. Bioeng. 43(11), 1994
PMID: 18615531
Xie L, Wang DI., Biotechnol. Bioeng. 43(11), 1994
PMID: 18615531
Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells.
Lohr V, Genzel Y, Jordan I, Katinger D, Mahr S, Sandig V, Reichl U., BMC Biotechnol. 12(), 2012
PMID: 23110398
Lohr V, Genzel Y, Jordan I, Katinger D, Mahr S, Sandig V, Reichl U., BMC Biotechnol. 12(), 2012
PMID: 23110398
Primary metabolism in the new human cell line AGE1.HN at various substrate levels: increased metabolic efficiency and α1-antitrypsin production at reduced pyruvate load.
Niklas J, Priesnitz C, Rose T, Sandig V, Heinzle E., Appl. Microbiol. Biotechnol. 93(4), 2011
PMID: 21842438
Niklas J, Priesnitz C, Rose T, Sandig V, Heinzle E., Appl. Microbiol. Biotechnol. 93(4), 2011
PMID: 21842438
The importance of ammonia in mammalian cell culture.
Schneider M, Marison IW, von Stockar U., J. Biotechnol. 46(3), 1996
PMID: 8672289
Schneider M, Marison IW, von Stockar U., J. Biotechnol. 46(3), 1996
PMID: 8672289
Effect of ammonia on the glycosylation of human recombinant erythropoietin in culture.
Yang M, Butler M., Biotechnol. Prog. 16(5), 2000
PMID: 11027166
Yang M, Butler M., Biotechnol. Prog. 16(5), 2000
PMID: 11027166
Influence of different ammonium, lactate and glutamine concentrations on CCO cell growth.
Slivac I, Blajic V, Radosevic K, Kniewald Z, Gaurina Srcek V., Cytotechnology 62(6), 2010
PMID: 21069459
Slivac I, Blajic V, Radosevic K, Kniewald Z, Gaurina Srcek V., Cytotechnology 62(6), 2010
PMID: 21069459
AUTHOR UNKNOWN, 1996
Glucose and glutamine metabolism of a murine B-lymphocyte hybridoma grown in batch culture.
Fitzpatrick L, Jenkins HA, Butler M., Appl. Biochem. Biotechnol. 43(2), 1993
PMID: 8267405
Fitzpatrick L, Jenkins HA, Butler M., Appl. Biochem. Biotechnol. 43(2), 1993
PMID: 8267405
Comparative analysis of glucose and glutamine metabolism in transformed mammalian cell lines, insect and primary liver cells.
Neermann J, Wagner R., J. Cell. Physiol. 166(1), 1996
PMID: 8557765
Neermann J, Wagner R., J. Cell. Physiol. 166(1), 1996
PMID: 8557765
Energy metabolism and ATP balance in animal cell cultivation using a stoichiometrically based reaction network.
Xie L, Wang DI., Biotechnol. Bioeng. 52(5), 1996
PMID: 18629932
Xie L, Wang DI., Biotechnol. Bioeng. 52(5), 1996
PMID: 18629932
A detailed metabolic flux analysis of an underdetermined network of CHO cells.
Zamorano F, Wouwer AV, Bastin G., J. Biotechnol. 150(4), 2010
PMID: 20869402
Zamorano F, Wouwer AV, Bastin G., J. Biotechnol. 150(4), 2010
PMID: 20869402
Metabolic adaptation of MDCK cells to different growth conditions: effects on catalytic activities of central metabolic enzymes.
Janke R, Genzel Y, Handel N, Wahl A, Reichl U., Biotechnol. Bioeng. 108(11), 2011
PMID: 21618469
Janke R, Genzel Y, Handel N, Wahl A, Reichl U., Biotechnol. Bioeng. 108(11), 2011
PMID: 21618469
Measurement of key metabolic enzyme activities in mammalian cells using rapid and sensitive microplate-based assays.
Janke R, Genzel Y, Wahl A, Reichl U., Biotechnol. Bioeng. 107(3), 2010
PMID: 20517988
Janke R, Genzel Y, Wahl A, Reichl U., Biotechnol. Bioeng. 107(3), 2010
PMID: 20517988
Growth, productivity and protein glycosylation in a CHO EpoFc producer cell line adapted to glutamine-free growth.
Taschwer M, Hackl M, Hernandez Bort JA, Leitner C, Kumar N, Puc U, Grass J, Papst M, Kunert R, Altmann F, Borth N., J. Biotechnol. 157(2), 2011
PMID: 22178781
Taschwer M, Hackl M, Hernandez Bort JA, Leitner C, Kumar N, Puc U, Grass J, Papst M, Kunert R, Altmann F, Borth N., J. Biotechnol. 157(2), 2011
PMID: 22178781
Methods for reducing the ammonia in hybridoma cell cultures.
Capiaumont J, Legrand C, Carbonell D, Dousset B, Belleville F, Nabet P., J. Biotechnol. 39(1), 1995
PMID: 7766010
Capiaumont J, Legrand C, Carbonell D, Dousset B, Belleville F, Nabet P., J. Biotechnol. 39(1), 1995
PMID: 7766010
Growth inhibition in animal cell culture. The effect of lactate and ammonia.
Hassell T, Gleave S, Butler M., Appl. Biochem. Biotechnol. 30(1), 1991
PMID: 1952924
Hassell T, Gleave S, Butler M., Appl. Biochem. Biotechnol. 30(1), 1991
PMID: 1952924
Stressing out over survival: glutamine as an apoptotic modulator.
Fuchs BC, Bode BP., J. Surg. Res. 131(1), 2005
PMID: 16154153
Fuchs BC, Bode BP., J. Surg. Res. 131(1), 2005
PMID: 16154153
Fibronectin-integrin signaling is required for L-glutamine's protection against gut injury.
Niederlechner S, Klawitter J, Baird C, Kallweit AR, Christians U, Wischmeyer PE., PLoS ONE 7(11), 2012
PMID: 23185570
Niederlechner S, Klawitter J, Baird C, Kallweit AR, Christians U, Wischmeyer PE., PLoS ONE 7(11), 2012
PMID: 23185570
Effects of amino acid additions on ammonium stressed CHO cells.
Chen P, Harcum SW., J. Biotechnol. 117(3), 2005
PMID: 15862358
Chen P, Harcum SW., J. Biotechnol. 117(3), 2005
PMID: 15862358
Substitution of glutamine by pyruvate to reduce ammonia formation and growth inhibition of mammalian cells.
Genzel Y, Ritter JB, Konig S, Alt R, Reichl U., Biotechnol. Prog. 21(1), 2005
PMID: 15903241
Genzel Y, Ritter JB, Konig S, Alt R, Reichl U., Biotechnol. Prog. 21(1), 2005
PMID: 15903241
Methods for off-line analysis of nutrients and products in mammalian cell culture
AUTHOR UNKNOWN, 2007
AUTHOR UNKNOWN, 2007
AUTHOR UNKNOWN, 1998
Chemical decomposition of glutamine in cell culture media: effect of media type, pH, and serum concentration.
Ozturk SS, Palsson BO., Biotechnol. Prog. 6(2), 1990
PMID: 1366484
Ozturk SS, Palsson BO., Biotechnol. Prog. 6(2), 1990
PMID: 1366484
Linear constraint relations in biochemical reaction systems: I. Classification of the calculability and the balanceability of conversion rates.
van der Heijden RT, Heijnen JJ, Hellinga C, Romein B, Luyben KC., Biotechnol. Bioeng. 43(1), 1994
PMID: 18613305
van der Heijden RT, Heijnen JJ, Hellinga C, Romein B, Luyben KC., Biotechnol. Bioeng. 43(1), 1994
PMID: 18613305
Calculability analysis in underdetermined metabolic networks illustrated by a model of the central metabolism in purple nonsulfur bacteria.
Klamt S, Schuster S, Gilles ED., Biotechnol. Bioeng. 77(7), 2002
PMID: 11835134
Klamt S, Schuster S, Gilles ED., Biotechnol. Bioeng. 77(7), 2002
PMID: 11835134
What is flux balance analysis?
Orth JD, Thiele I, Palsson BO., Nat. Biotechnol. 28(3), 2010
PMID: 20212490
Orth JD, Thiele I, Palsson BO., Nat. Biotechnol. 28(3), 2010
PMID: 20212490
An interval approach for dealing with flux distributions and elementary modes activity patterns.
Llaneras F, Pico J., J. Theor. Biol. 246(2), 2007
PMID: 17292923
Llaneras F, Pico J., J. Theor. Biol. 246(2), 2007
PMID: 17292923
Examination of primary metabolic pathways in a murine hybridoma with carbon-13 nuclear magnetic resonance spectroscopy.
Mancuso A, Sharfstein ST, Tucker SN, Clark DS, Blanch HW., Biotechnol. Bioeng. 44(5), 1994
PMID: 18618793
Mancuso A, Sharfstein ST, Tucker SN, Clark DS, Blanch HW., Biotechnol. Bioeng. 44(5), 1994
PMID: 18618793
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