The SNARE protein vti1a functions in dense-core vesicle biogenesis

Walter AM, Kurps J, de Wit H, Schoening S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, et al. (2014)
The EMBO Journal 33(15): 1681-1697.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Walter, Alexander M.; Kurps, Julia; de Wit, Heidi; Schoening, Susanne; Toft-Bertelsen, Trine L.; Lauks, Juliane; Ziomkiewicz, Iwona; Weiss, Annita N.; Schulz, Alexander; Fischer von Mollard, GabrieleUniBi ; Verhage, Matthijs; Sorensen, Jakob B.
Abstract / Bemerkung
The SNARE protein vti1a is proposed to drive fusion of intracellular organelles, but recent data also implicated vti1a in exocytosis. Here we show that vti1a is absent from mature secretory vesicles in adrenal chromaffin cells, but localizes to a compartment near the trans-Golgi network, partially overlapping with syntaxin-6. Exocytosis is impaired in vti1a null cells, partly due to fewer Ca2+-channels at the plasma membrane, partly due to fewer vesicles of reduced size and synaptobrevin-2 content. In contrast, release kinetics and Ca2+-sensitivity remain unchanged, indicating that the final fusion reaction leading to transmitter release is unperturbed. Additional deletion of the closest related SNARE, vti1b, does not exacerbate the vti1a phenotype, and vti1b null cells show no secretion defects, indicating that vti1b does not participate in exocytosis. Long-term re-expression of vti1a (days) was necessary for restoration of secretory capacity, whereas strong short-term expression (hours) was ineffective, consistent with vti1a involvement in an upstream step related to vesicle generation, rather than in fusion. We conclude that vti1a functions in vesicle generation and Ca2+-channel trafficking, but is dispensable for transmitter release.
adrenal chromaffin cells; SNARE; Ca2+-channels exocytosis; vesicle; biogenesis
The EMBO Journal
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Walter AM, Kurps J, de Wit H, et al. The SNARE protein vti1a functions in dense-core vesicle biogenesis. The EMBO Journal. 2014;33(15):1681-1697.
Walter, A. M., Kurps, J., de Wit, H., Schoening, S., Toft-Bertelsen, T. L., Lauks, J., Ziomkiewicz, I., et al. (2014). The SNARE protein vti1a functions in dense-core vesicle biogenesis. The EMBO Journal, 33(15), 1681-1697. doi:10.15252/embj.201387549
Walter, Alexander M., Kurps, Julia, de Wit, Heidi, Schoening, Susanne, Toft-Bertelsen, Trine L., Lauks, Juliane, Ziomkiewicz, Iwona, et al. 2014. “The SNARE protein vti1a functions in dense-core vesicle biogenesis”. The EMBO Journal 33 (15): 1681-1697.
Walter, A. M., Kurps, J., de Wit, H., Schoening, S., Toft-Bertelsen, T. L., Lauks, J., Ziomkiewicz, I., Weiss, A. N., Schulz, A., Fischer von Mollard, G., et al. (2014). The SNARE protein vti1a functions in dense-core vesicle biogenesis. The EMBO Journal 33, 1681-1697.
Walter, A.M., et al., 2014. The SNARE protein vti1a functions in dense-core vesicle biogenesis. The EMBO Journal, 33(15), p 1681-1697.
A.M. Walter, et al., “The SNARE protein vti1a functions in dense-core vesicle biogenesis”, The EMBO Journal, vol. 33, 2014, pp. 1681-1697.
Walter, A.M., Kurps, J., de Wit, H., Schoening, S., Toft-Bertelsen, T.L., Lauks, J., Ziomkiewicz, I., Weiss, A.N., Schulz, A., Fischer von Mollard, G., Verhage, M., Sorensen, J.B.: The SNARE protein vti1a functions in dense-core vesicle biogenesis. The EMBO Journal. 33, 1681-1697 (2014).
Walter, Alexander M., Kurps, Julia, de Wit, Heidi, Schoening, Susanne, Toft-Bertelsen, Trine L., Lauks, Juliane, Ziomkiewicz, Iwona, Weiss, Annita N., Schulz, Alexander, Fischer von Mollard, Gabriele, Verhage, Matthijs, and Sorensen, Jakob B. “The SNARE protein vti1a functions in dense-core vesicle biogenesis”. The EMBO Journal 33.15 (2014): 1681-1697.

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