Hydrodynamic Slip on DNA Observed by Optical Tweezers-Controlled Translocation Experiments with Solid-State and Lipid-Coated Nanopores

Galla L, Meyer A, Spiering A, Sischka A, Mayer M, Hall AR, Reimann P, Anselmetti D (2014)
Nano Letters 14(7): 4176-4182.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
We use optical tweezers to investigate the threading force on a single dsDNA molecule inside silicon-nitride nanopores between 6 and 70 nm in diameter, as well as lipid-coated solid-state nanopores. We observe a strong increase of the threading force for decreasing nanopore size that can be attributed to a significant reduction in the electroosmotic flow (EOF), which opposes the electrophoresis. Additionally, we show that the EOF can also be reduced by coating the nanopore wall with an electrically neutral lipid bilayer, resulting in an 85% increase in threading force. All experimental findings can be described by a quantitative theoretical model that incorporates a hydrodynamic slip effect on the DNA surface with a slip length of 0.5 nm.
Stichworte
DNA translocation; Lipid-coated solid-state nanopores; optical tweezers; slip length; electroosmotic flow
Erscheinungsjahr
2014
Zeitschriftentitel
Nano Letters
Band
14
Ausgabe
7
Seite(n)
4176-4182
ISSN
1530-6984
eISSN
1530-6992
Page URI
https://pub.uni-bielefeld.de/record/2690821

Zitieren

Galla L, Meyer A, Spiering A, et al. Hydrodynamic Slip on DNA Observed by Optical Tweezers-Controlled Translocation Experiments with Solid-State and Lipid-Coated Nanopores. Nano Letters. 2014;14(7):4176-4182.
Galla, L., Meyer, A., Spiering, A., Sischka, A., Mayer, M., Hall, A. R., Reimann, P., et al. (2014). Hydrodynamic Slip on DNA Observed by Optical Tweezers-Controlled Translocation Experiments with Solid-State and Lipid-Coated Nanopores. Nano Letters, 14(7), 4176-4182. doi:10.1021/nl501909t
Galla, L., Meyer, A., Spiering, A., Sischka, A., Mayer, M., Hall, A. R., Reimann, P., and Anselmetti, D. (2014). Hydrodynamic Slip on DNA Observed by Optical Tweezers-Controlled Translocation Experiments with Solid-State and Lipid-Coated Nanopores. Nano Letters 14, 4176-4182.
Galla, L., et al., 2014. Hydrodynamic Slip on DNA Observed by Optical Tweezers-Controlled Translocation Experiments with Solid-State and Lipid-Coated Nanopores. Nano Letters, 14(7), p 4176-4182.
L. Galla, et al., “Hydrodynamic Slip on DNA Observed by Optical Tweezers-Controlled Translocation Experiments with Solid-State and Lipid-Coated Nanopores”, Nano Letters, vol. 14, 2014, pp. 4176-4182.
Galla, L., Meyer, A., Spiering, A., Sischka, A., Mayer, M., Hall, A.R., Reimann, P., Anselmetti, D.: Hydrodynamic Slip on DNA Observed by Optical Tweezers-Controlled Translocation Experiments with Solid-State and Lipid-Coated Nanopores. Nano Letters. 14, 4176-4182 (2014).
Galla, Lukas, Meyer, Andreas, Spiering, André, Sischka, Andy, Mayer, Michael, Hall, Adam R., Reimann, Peter, and Anselmetti, Dario. “Hydrodynamic Slip on DNA Observed by Optical Tweezers-Controlled Translocation Experiments with Solid-State and Lipid-Coated Nanopores”. Nano Letters 14.7 (2014): 4176-4182.

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