Structural diversity of polyoxomolybdate clusters along the three-fold axis of the molybdenum storage protein

Poppe J, Warkentin E, Demmer U, Kowalewski B, Dierks T, Schneider K, Ermler U (2014)
Journal of Inorganic Biochemistry 138: 122-128.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Poppe, Juliane; Warkentin, Eberhard; Demmer, Ulrike; Kowalewski, BjörnUniBi; Dierks, ThomasUniBi; Schneider, KlausUniBi; Ermler, Ulrich
Abstract / Bemerkung
The molybdenum storage protein (MoSto) can store more than 100 Mo or W atoms as discrete polyoxometalate (POM) clusters. Here, we describe the three POM cluster sites along the threefold axis of the protein complex based on four X-ray structures with slightly different polyoxomolybdate compositions between 1.35 and 2 angstrom resolution. In contrast to the Mo alpha-out binding site occupied by an Mo-3 cluster, the Mo alpha-in and Mo-beta binding sites contain rather weak and non-uniform electron density for the Mo atoms (but clearly identifiable by anomalous data), suggesting the presence of POM cluster ensembles and/or degradation products of larger aggregates. The "Mo alpha-in cluster ensemble" was interpreted as an antiprism-like Mo-6 species superimposed with an Mo-7 pyramide and the "Mo-beta cluster ensemble" as an Mo-13 cluster (present mostly ins degraded form) composed of a pyramidal Mo-7 and a Mo-3 building block linked by three spatially separated MoOx units. Inside the ball-shaped Mo-13 cluster sits an occluded central atom, perhaps a metal ion. POM cluster formation at the Mo alpha-in, and Mo-beta sites appears to be driven by filtering out and binding/protecting self-assembled transient species complementary to the protein template. (C) 2014 Elsevier Inc. All rights reserved.
Stichworte
Azotobacter vinelandii; Molybdenum storage protein; Polyoxometalate; Template; Crystal structure
Erscheinungsjahr
2014
Zeitschriftentitel
Journal of Inorganic Biochemistry
Band
138
Seite(n)
122-128
ISSN
0162-0134
Page URI
https://pub.uni-bielefeld.de/record/2690696

Zitieren

Poppe J, Warkentin E, Demmer U, et al. Structural diversity of polyoxomolybdate clusters along the three-fold axis of the molybdenum storage protein. Journal of Inorganic Biochemistry. 2014;138:122-128.
Poppe, J., Warkentin, E., Demmer, U., Kowalewski, B., Dierks, T., Schneider, K., & Ermler, U. (2014). Structural diversity of polyoxomolybdate clusters along the three-fold axis of the molybdenum storage protein. Journal of Inorganic Biochemistry, 138, 122-128. doi:10.1016/j.jinorgbio.2014.05.009
Poppe, J., Warkentin, E., Demmer, U., Kowalewski, B., Dierks, T., Schneider, K., and Ermler, U. (2014). Structural diversity of polyoxomolybdate clusters along the three-fold axis of the molybdenum storage protein. Journal of Inorganic Biochemistry 138, 122-128.
Poppe, J., et al., 2014. Structural diversity of polyoxomolybdate clusters along the three-fold axis of the molybdenum storage protein. Journal of Inorganic Biochemistry, 138, p 122-128.
J. Poppe, et al., “Structural diversity of polyoxomolybdate clusters along the three-fold axis of the molybdenum storage protein”, Journal of Inorganic Biochemistry, vol. 138, 2014, pp. 122-128.
Poppe, J., Warkentin, E., Demmer, U., Kowalewski, B., Dierks, T., Schneider, K., Ermler, U.: Structural diversity of polyoxomolybdate clusters along the three-fold axis of the molybdenum storage protein. Journal of Inorganic Biochemistry. 138, 122-128 (2014).
Poppe, Juliane, Warkentin, Eberhard, Demmer, Ulrike, Kowalewski, Björn, Dierks, Thomas, Schneider, Klaus, and Ermler, Ulrich. “Structural diversity of polyoxomolybdate clusters along the three-fold axis of the molybdenum storage protein”. Journal of Inorganic Biochemistry 138 (2014): 122-128.

6 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Genetic and Biochemical Analysis of the Azotobacter vinelandii Molybdenum Storage Protein.
Navarro-Rodríguez M, Buesa JM, Rubio LM., Front Microbiol 10(), 2019
PMID: 30984129
Polyoxometalates: more than a phasing tool in protein crystallography.
Bijelic A, Rompel A., ChemTexts 4(3), 2018
PMID: 30596006
Production and isolation of vanadium nitrogenase from Azotobacter vinelandii by molybdenum depletion.
Sippel D, Schlesier J, Rohde M, Trncik C, Decamps L, Djurdjevic I, Spatzal T, Andrade SL, Einsle O., J Biol Inorg Chem 22(1), 2017
PMID: 27928630
A survey of the different roles of polyoxometalates in their interaction with amino acids, peptides and proteins.
Arefian M, Mirzaei M, Eshtiagh-Hosseini H, Frontera A., Dalton Trans 46(21), 2017
PMID: 28530742

42 References

Daten bereitgestellt von Europe PubMed Central.


Pope, Prog. Inorg. Chem. 30(), 1991

AUTHOR UNKNOWN, 2004

Cronin, 2004

Cindrić, Croat. Chem. Acta 82(), 2009
Polyoxometalates: building blocks for functional nanoscale systems.
Long DL, Tsunashima R, Cronin L., Angew. Chem. Int. Ed. Engl. 49(10), 2010
PMID: 20131346

Gouzerh, L'actualité Chim. 298(), 2006
Inorganic chemistry goes protein size: a Mo368 nano-hedgehog initiating nanochemistry by symmetry breaking.
Muller A, Beckmann E, Bogge H, Schmidtmann M, Dress A., Angew. Chem. Int. Ed. Engl. 41(7), 2002
PMID: 12491247

Müller, Polyhedron 23(), 2004
Nanohybridization of polyoxometalate clusters and single-wall carbon nanotubes: applications in molecular cluster batteries.
Kawasaki N, Wang H, Nakanishi R, Hamanaka S, Kitaura R, Shinohara H, Yokoyama T, Yoshikawa H, Awaga K., Angew. Chem. Int. Ed. Engl. 50(15), 2011
PMID: 21387508
Polyoxometalates in Medicine.
Rhule JT, Hill CL, Judd DA, Schinazi RF., Chem. Rev. 98(1), 1998
PMID: 11851509

Pope, Angew. Chem. Int. Ed. Engl. 30(), 1991

Müller, Angew. Chem. Int. Ed. Engl. 30(), 1991

Müller, Angew. Chem. Int. Ed. Engl. 34(), 1995

Hutin, Isr. J. Chem. 51(), 2011

Romao, Dalton Trans. (), 2009

Wever, 2001
Mechanism of Mo-dependent nitrogenase.
Seefeldt LC, Hoffman BM, Dean DR., Annu. Rev. Biochem. 78(), 2009
PMID: 19489731
Catalysis at a dinuclear [CuSMo(==O)OH] cluster in a CO dehydrogenase resolved at 1.1-A resolution.
Dobbek H, Gremer L, Kiefersauer R, Huber R, Meyer O., Proc. Natl. Acad. Sci. U.S.A. 99(25), 2002
PMID: 12475995
A new type of metalloprotein: The Mo storage protein from azotobacter vinelandii contains a polynuclear molybdenum-oxide cluster.
Fenske D, Gnida M, Schneider K, Meyer-Klaucke W, Schemberg J, Henschel V, Meyer AK, Knochel A, Muller A., Chembiochem 6(2), 2005
PMID: 15651045
Towards biological supramolecular chemistry: a variety of pocket-templated, individual metal oxide cluster nucleations in the cavity of a mo/w-storage protein.
Schemberg J, Schneider K, Demmer U, Warkentin E, Muller A, Ermler U., Angew. Chem. Int. Ed. Engl. 46(14), 2007
PMID: 17304608
Selective removal of molybdenum traces from growth media of N2-fixing bacteria.
Schneider K, Muller A, Johannes KU, Diemann E, Kottmann J., Anal. Biochem. 193(2), 1991
PMID: 1908197
Structural Basis of Biological Nitrogen Fixation.
Howard JB, Rees DC., Chem. Rev. 96(7), 1996
PMID: 11848848
Molybdenum cofactors, enzymes and pathways.
Schwarz G, Mendel RR, Ribbe MW., Nature 460(7257), 2009
PMID: 19675644
Molybdenum trafficking for nitrogen fixation.
Hernandez JA, George SJ, Rubio LM., Biochemistry 48(41), 2009
PMID: 19772354
Nature's polyoxometalate chemistry: X-ray structure of the Mo storage protein loaded with discrete polynuclear Mo-O clusters.
Kowalewski B, Poppe J, Demmer U, Warkentin E, Dierks T, Ermler U, Schneider K., J. Am. Chem. Soc. 134(23), 2012
PMID: 22612644
Formation of the nitrogen-fixing enzyme system in Azotobacter vinelandii.
Strandberg GW, Wilson PW., Can. J. Microbiol. 14(1), 1968
PMID: 5644401

Pantaler, J. Anal. Chem. USSR 18(), 1963
XDS.
Kabsch W., Acta Crystallogr. D Biol. Crystallogr. 66(Pt 2), 2010
PMID: 20124692
Refinement of macromolecular structures by the maximum-likelihood method.
Murshudov GN, Vagin AA, Dodson EJ., Acta Crystallogr. D Biol. Crystallogr. 53(Pt 3), 1997
PMID: 15299926
phenix.model_vs_data: a high-level tool for the calculation of crystallographic model and data statistics.
Afonine PV, Grosse-Kunstleve RW, Chen VB, Headd JJ, Moriarty NW, Richardson JS, Richardson DC, Urzhumtsev A, Zwart PH, Adams PD., J Appl Crystallogr 43(Pt 4), 2010
PMID: 20648263
Coot: model-building tools for molecular graphics.
Emsley P, Cowtan K., Acta Crystallogr. D Biol. Crystallogr. 60(Pt 12 Pt 1), 2004
PMID: 15572765
The CCP4 molecular-graphics project.
Potterton E, McNicholas S, Krissinel E, Cowtan K, Noble M., Acta Crystallogr. D Biol. Crystallogr. 58(Pt 11), 2002
PMID: 12393928
Escherichia coli UMP-kinase, a member of the aspartokinase family, is a hexamer regulated by guanine nucleotides and UTP.
Serina L, Blondin C, Krin E, Sismeiro O, Danchin A, Sakamoto H, Gilles AM, Barzu O., Biochemistry 34(15), 1995
PMID: 7711027

Wu, Polyhedron 22(), 2003

Dawson, Acta Crystallogr. 6(), 1953

Anderson, Nature 140(), 1937

Lipscomb, Inorg. Chem. 4(), 1965

Müller, Coord. Chem. Rev. 182(), 1999

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 24945101
PubMed | Europe PMC

Suchen in

Google Scholar