Stable nuclear transformation of Pandorina morum

Lerche K, Hallmann A (2014)
BMC Biotechnology 14(1): 65.

Download
OA
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Volltext vorhanden für diesen Nachweis
Abstract / Bemerkung
Background Volvocine green algae like Pandorina morum represent one of the most recent inventions of multicellularity diverged from their unicellular relatives. The 8–16 celled P. morum alga and its close multicellular relatives constitute a model lineage for research into cellular differentiation, morphogenesis and epithelial folding, sexual reproduction and evolution of multicellularity. Pandorina is the largest and most complex organism in the volvocine lineage that still exhibits isogamous sexual reproduction. So far, molecular-biological investigations in P. morum were constricted due to the absence of methods for transformation of this species, which is a prerequisite for introduction of reporter genes and (modified) genes of interest. Results Stable nuclear transformation of P. morum was achieved using chimeric constructs with a selectable marker, a reporter gene, promoters and upstream and downstream flanking sequences from heterologous sources. DNA was introduced into the cells by particle bombardment with plasmid-coated gold particles. The aminoglycoside 3′-phosphotransferase VIII (aphVIII) gene of Streptomyces rimosus under control of an artificial, heterologous promoter was used as the selectable marker. The artificial promoter contained a tandem arrangement of the promoter of both the heat shock protein 70A (hsp70A) and the ribulose-1,5-bisphosphat-carboxylase/-oxygenase S3 (rbcS3) gene of Volvox carteri. Due to the expression of aphVIII, transformants gained up to 333-fold higher resistance to paromomycin in comparison to the parent wild-type strain. The heterologous luciferase (gluc) gene of Gaussia princeps, which was previously genetically engineered to match the nuclear codon usage of Chlamydomonas reinhardtii, was used as a co-transformed, unselectable reporter gene. The expression of the co-bombarded gluc gene in transformants and the induction of gluc by heat shock were demonstrated through bioluminescence assays. Conclusion Stable nuclear transformation of P. morum using the particle bombardment technique is now feasible. Functional expression of heterologous genes is achieved using heterologous flanking sequences from Volvox carteri and Chlamydomonas reinhardtii. The aphVIII gene of the actinobacterium S. rimosus can be used as a selectable marker for transformation experiments in the green alga P. morum. The gluc gene of the marine copepod G. princeps, expressed under control of heterologous promoter elements, represents a suitable reporter gene for monitoring gene expression or for other applications in P. morum.
Erscheinungsjahr
Zeitschriftentitel
BMC Biotechnology
Band
14
Ausgabe
1
Seite(n)
65
ISSN
PUB-ID

Zitieren

Lerche K, Hallmann A. Stable nuclear transformation of Pandorina morum. BMC Biotechnology. 2014;14(1):65.
Lerche, K., & Hallmann, A. (2014). Stable nuclear transformation of Pandorina morum. BMC Biotechnology, 14(1), 65. doi:10.1186/1472-6750-14-65
Lerche, K., and Hallmann, A. (2014). Stable nuclear transformation of Pandorina morum. BMC Biotechnology 14, 65.
Lerche, K., & Hallmann, A., 2014. Stable nuclear transformation of Pandorina morum. BMC Biotechnology, 14(1), p 65.
K. Lerche and A. Hallmann, “Stable nuclear transformation of Pandorina morum”, BMC Biotechnology, vol. 14, 2014, pp. 65.
Lerche, K., Hallmann, A.: Stable nuclear transformation of Pandorina morum. BMC Biotechnology. 14, 65 (2014).
Lerche, Kai, and Hallmann, Armin. “Stable nuclear transformation of Pandorina morum”. BMC Biotechnology 14.1 (2014): 65.
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2014-08-14T08:55:45Z

6 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Two-component cyclase opsins of green algae are ATP-dependent and light-inhibited guanylyl cyclases.
Tian Y, Gao S, von der Heyde EL, Hallmann A, Nagel G., BMC Biol 16(1), 2018
PMID: 30522480
The inducible nitA promoter provides a powerful molecular switch for transgene expression in Volvox carteri.
von der Heyde EL, Klein B, Abram L, Hallmann A., BMC Biotechnol 15(), 2015
PMID: 25888095
Understanding nitrate assimilation and its regulation in microalgae.
Sanz-Luque E, Chamizo-Ampudia A, Llamas A, Galvan A, Fernandez E., Front Plant Sci 6(), 2015
PMID: 26579149

47 References

Daten bereitgestellt von Europe PubMed Central.

Triassic origin and early radiation of multicellular volvocine algae.
Herron MD, Hackett JD, Aylward FO, Michod RE., Proc. Natl. Acad. Sci. U.S.A. 106(9), 2009
PMID: 19223580

AUTHOR UNKNOWN, 1998
Evolution of reproductive development in the volvocine algae.
Hallmann A., Sex. Plant Reprod. 24(2), 2010
PMID: 21174128

AUTHOR UNKNOWN, 2009

AUTHOR UNKNOWN, 1824
Ultrastructure of the extracellular matrix and taxonomy of Eudorina, Pleodorina and Yamagishiella gen. nov. (Volvocaceae, Chlorophyta)
AUTHOR UNKNOWN, 1992
The sexual process of Japanese Pandorina morum Bory (Chlorophyta)
AUTHOR UNKNOWN, 1979
Stable nuclear transformation of Eudorina elegans.
Lerche K, Hallmann A., BMC Biotechnol. 13(), 2013
PMID: 23402598
The linear 20 kb mitochondrial genome of Pandorina morum (Volvocaceae, Chlorophyta).
Moore LJ, Coleman AW., Plant Mol. Biol. 13(4), 1989
PMID: 2491666
The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, Terry A, Salamov A, Fritz-Laylin LK, Marechal-Drouard L, Marshall WF, Qu LH, Nelson DR, Sanderfoot AA, Spalding MH, Kapitonov VV, Ren Q, Ferris P, Lindquist E, Shapiro H, Lucas SM, Grimwood J, Schmutz J, Cardol P, Cerutti H, Chanfreau G, Chen CL, Cognat V, Croft MT, Dent R, Dutcher S, Fernandez E, Fukuzawa H, Gonzalez-Ballester D, Gonzalez-Halphen D, Hallmann A, Hanikenne M, Hippler M, Inwood W, Jabbari K, Kalanon M, Kuras R, Lefebvre PA, Lemaire SD, Lobanov AV, Lohr M, Manuell A, Meier I, Mets L, Mittag M, Mittelmeier T, Moroney JV, Moseley J, Napoli C, Nedelcu AM, Niyogi K, Novoselov SV, Paulsen IT, Pazour G, Purton S, Ral JP, Riano-Pachon DM, Riekhof W, Rymarquis L, Schroda M, Stern D, Umen J, Willows R, Wilson N, Zimmer SL, Allmer J, Balk J, Bisova K, Chen CJ, Elias M, Gendler K, Hauser C, Lamb MR, Ledford H, Long JC, Minagawa J, Page MD, Pan J, Pootakham W, Roje S, Rose A, Stahlberg E, Terauchi AM, Yang P, Ball S, Bowler C, Dieckmann CL, Gladyshev VN, Green P, Jorgensen R, Mayfield S, Mueller-Roeber B, Rajamani S, Sayre RT, Brokstein P, Dubchak I, Goodstein D, Hornick L, Huang YW, Jhaveri J, Luo Y, Martinez D, Ngau WC, Otillar B, Poliakov A, Porter A, Szajkowski L, Werner G, Zhou K, Grigoriev IV, Rokhsar DS, Grossman AR., Science 318(5848), 2007
PMID: 17932292
Genomic analysis of organismal complexity in the multicellular green alga Volvox carteri.
Prochnik SE, Umen J, Nedelcu AM, Hallmann A, Miller SM, Nishii I, Ferris P, Kuo A, Mitros T, Fritz-Laylin LK, Hellsten U, Chapman J, Simakov O, Rensing SA, Terry A, Pangilinan J, Kapitonov V, Jurka J, Salamov A, Shapiro H, Schmutz J, Grimwood J, Lindquist E, Lucas S, Grigoriev IV, Schmitt R, Kirk D, Rokhsar DS., Science 329(5988), 2010
PMID: 20616280
Nuclear transformation of Volvox carteri.
Schiedlmeier B, Schmitt R, Muller W, Kirk MM, Gruber H, Mages W, Kirk DL., Proc. Natl. Acad. Sci. U.S.A. 91(11), 1994
PMID: 8197189
High-frequency nuclear transformation of Chlamydomonas reinhardtii.
Kindle KL., Proc. Natl. Acad. Sci. U.S.A. 87(3), 1990
PMID: 2105499
High-velocity microprojectiles for delivering nucleic acids into living cells. 1987.
Klein RM, Wolf ED, Wu R, Sanford JC., Biotechnology 24(), 1992
PMID: 1422046
Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation.
Brown LE, Sprecher SL, Keller LR., Mol. Cell. Biol. 11(4), 1991
PMID: 2005916
Genetic transformation of the green alga--Chlamydononas reinhardtii by Agrobacterium tumefaciens.
Kumar SV, Misquitta RW, Reddy VS, Rao BJ, Rajam MV., Plant Sci. 166(3), 2004
PMID: IND43633164
Stable nuclear transformation of Gonium pectorale.
Lerche K, Hallmann A., BMC Biotechnol. 9(), 2009
PMID: 19591675
Isolation and characterization of a mutant protoporphyrinogen oxidase gene from Chlamydomonas reinhardtii conferring resistance to porphyric herbicides.
Randolph-Anderson BL, Sato R, Johnson AM, Harris EH, Hauser CR, Oeda K, Ishige F, Nishio S, Gillham NW, Boynton JE., Plant Mol. Biol. 38(5), 1998
PMID: 9862501
The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas.
Stevens DR, Rochaix JD, Purton S., Mol. Gen. Genet. 251(1), 1996
PMID: 8628243
Algal transgenics and biotechnology
AUTHOR UNKNOWN, 2007
The bacterial paromomycin resistance gene, aphH, as a dominant selectable marker in Volvox carteri.
Jakobiak T, Mages W, Scharf B, Babinger P, Stark K, Schmitt R., Protist 155(4), 2004
PMID: 15648719
Bacterial resistance to aminoglycoside antibiotics.
Davies J, Wright GD., Trends Microbiol. 5(6), 1997
PMID: 9211644
Bioluminescence in the mesopelagic copepod, Gaussia princeps (T. Scott)
AUTHOR UNKNOWN, 1972
Cloning and characterization of new bioluminescent proteins
AUTHOR UNKNOWN, 1999
Enzymes involved in anaerobic respiration appear to play a role in Actinobacillus pleuropneumoniae virulence.
Jacobsen I, Hennig-Pauka I, Baltes N, Trost M, Gerlach GF., Infect. Immun. 73(1), 2005
PMID: 15618158
Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase.
Kindle KL, Schnell RA, Fernandez E, Lefebvre PA., J. Cell Biol. 109(6 Pt 1), 1989
PMID: 2592399
Stable nuclear transformation of Chlamydomonas reinhardtii with a Streptomyces rimosus gene as the selective marker.
Sizova IA, Lapina TV, Frolova ON, Alexandrova NN, Akopiants KE, Danilenko VN., Gene 181(1-2), 1996
PMID: 8973302
Stable nuclear transformation of the diatom Phaeodactylum tricornutum.
Apt KE, Kroth-Pancic PG, Grossman AR., Mol. Gen. Genet. 252(5), 1996
PMID: 8914518
Genetic transformation of the diatoms Cyclotella cryptica and Navicula saprophila
AUTHOR UNKNOWN, 1995
Reporter genes and highly regulated promoters as tools for transformation experiments in Volvox carteri.
Hallmann A, Sumper M., Proc. Natl. Acad. Sci. U.S.A. 91(24), 1994
PMID: 7972102
Gene replacement by homologous recombination in the multicellular green alga Volvox carteri.
Hallmann A, Rappel A, Sumper M., Proc. Natl. Acad. Sci. U.S.A. 94(14), 1997
PMID: 9207115

AUTHOR UNKNOWN, 1988

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 25031031
PubMed | Europe PMC

Suchen in

Google Scholar